Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PTPMT1 CRISPR/Cas9 KO Plasmid (h): sc-403905
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PTPMT1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PTPMT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PTPMT1 Antibody (B-3): sc-390901
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PTPMT1 CRISPR/Cas9 KO Plasmid (h) | sc-403905 | 20 µg | $397.00 |
Overview
PTPMT1 encodes a mitochondria-localized protein tyrosine phosphatase that dephosphorylates phosphatidylglycerophosphate to support cardiolipin biosynthesis and inner mitochondrial membrane integrity. By regulating cardiolipin-dependent organization of respiratory chain supercomplexes, PTPMT1 influences oxidative phosphorylation, mitochondrial dynamics, and cellular energy homeostasis. Its activity intersects with lipid remodeling and mitochondrial stress-response pathways that shape proliferation and survival in metabolically demanding cells. Dysregulation of PTPMT1-linked mitochondrial lipid metabolism has been associated with altered bioenergetics and redox balance, making it relevant for studies of metabolic adaptation and mitochondrial dysfunction.
PTPMT1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PTPMT1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PTPMT1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PTPMT1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PTPMT1 protein expression.
This CRISPR knockout system enables efficient generation of PTPMT1-deficient cell models for investigation of PTPMT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PTPMT1 exon(s) critical for PTPMT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PTPMT1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PTPMT1 CRISPR/Cas9 KO Plasmid (h) and PTPMT1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PTPMT1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PTPMT1 HDR Plasmid (h) and PTPMT1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PTPMT1 homology arms to support homology-directed repair at defined PTPMT1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.