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PTPκ Antibody (H-3) | SCBT - Santa Cruz Biotechnology
PTP kappa抗体 (H-3) 推荐用于 mouse, rat 和 human 来源的PTPκ WB, IP, IF 和 ELISA检测

PTPκ 抗体 (H-3): sc-374315

PTPκ抗体 (H-3) is rated 4.8 out of 5 by 4.
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说明书

    • PTP kappa 抗体 H-3 是小鼠单克隆 IgG2b κ,PTPκ抗体, 在4篇文献中引用,规格为200 µg/ml
    • 免疫human来源PTPκ位于N-terminal胞外域的27-101氨基酸
    • PTP kappa 抗体 (H-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 PTPκ; 也和以下物种反应,包括: 和 bovine and porcine
    • 抗-PTP kappa 抗体 (H-3) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取PTPκ (H-3): sc-374315免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 PTPκ Antibody (H-3) for WB 首选应用二抗. 这些试剂现在以套形式提供 PTPκ Antibody (H-3) (见下方订购信息).
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What is the recommended fixation method for immunofluorescence staining with PTPκ (H-3): sc-374315 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good western blot detection We used this antibody for expression detection with western blot. We got good bands at the correct molecular weight
Date published: 2017-01-31
Rated 4 out of 5 by from Very good antibody for Western blotting This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and fairly specific antibody for Western blotting with lysates from diverse human cell lines. In immunoblots with a mix of A431, HepG2 and MCF7 cell lysates, this antibody strongly detects a ~150 kDa protein, which is slightly lower than the predicted size of ~162 kDa, but likely to be the intended target. This antibody also very strongly cross-reacts with a ~37 kDa protein in these cell lysates.
Date published: 2016-10-13
Rated 5 out of 5 by from Superb immunofluorescence cytoplasmic and Superb immunofluorescence cytoplasmic and membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-12
Rated 5 out of 5 by from Produced positive Western Blot data of PTP Produced positive Western Blot data of PTP expression in c4 whole cell lysate. -SCBT QC
Date published: 2014-07-17
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