Date published: 2025-9-7
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PPARβ Antibody (F-10) - Western Blotting - Image 137644
PPARβ (F-10): sc-74517. Sol8 (A), RAW 264. 7 (B), HeLa (C) 和 K-562 (D) 全细胞裂解液中 PPARβ 表达的 Western 印迹分析.
PPARβ (F-10): sc-74517. Sol8 (A), RAW 264. 7 (B), HeLa (C) 和 K-562 (D) 全细胞裂解液中 PPARβ 表达的 Western 印迹分析.

PPARβ 抗体 (F-10): sc-74517

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寫評論提問

说明书
  • PPAR beta 抗体 F-10 是小鼠单克隆 IgG1 κ，PPARβ抗体, 在54篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的PPARβ的氨基酸2-75
  • PPAR beta 抗体 (F-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 PPARβ
  • 抗PPAR beta抗体（F-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-74517 X, 200 µg/0.1 ml)
  • 关于如何获取PPARβ (F-10): sc-74517的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是PPARβ Antibody (F-10) for WB applications. 的首选辅助检测试剂，这些试剂现与PPARβ Antibody (F-10) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加PPAR抗体，包括PPAR α, PPAR β, PPAR γ和PPAR γ 2

PPARβ抗体（F-10）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的PPARβ蛋白。PPARβ (F-10) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。过氧化物酶体增殖物激活受体β（PPARβ），也称为PPARδ，是核激素受体超家族的一员，在调节与脂质代谢、炎症和细胞分化相关的基因表达方面发挥着至关重要的作用。PPARβ与类维生素X受体（RXRs）形成异二聚体，并与称为PPAR反应元件（PPREs）的特定DNA序列结合，这对靶基因的转录调控至关重要。这种相互作用在骨骼肌和脂肪组织等组织中尤为重要，PPARβ在这些组织中影响脂肪酸氧化和能量平衡。PPARβ参与多种生理过程，包括伤口愈合和炎症反应的调节，因此PPARβ成为代谢紊乱和心血管疾病治疗干预的重要靶点。抗PPARβ抗体（F-10）在多种应用中的多功能性使研究人员能够探索PPARβ在各种生物学环境中的不同功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PPARβ 抗体 (F-10) 参考文献:

  1. 引导配体进入核受体  |  Michalik, L. and Wahli, W. 2007. Cell. 129: 649-51. PMID: 17512397
  2. 过氧化物酶体增殖激活受体β的生理功能。  |  Neels, JG. and Grimaldi, PA. 2014. Physiol Rev. 94: 795-858. PMID: 24987006
  3. 揭示 PPARβ/δ 对胰岛素抵抗和心血管疾病的影响。  |  Vázquez-Carrera, M. 2016. Trends Endocrinol Metab. 27: 319-334. PMID: 27005447
  4. 过氧化物酶体增殖激活受体-β/δ在皮肤癌中的调控机制。  |  Peters, JM., et al. 2019. Mol Carcinog. 58: 1612-1622. PMID: 31062422
  5. 揭示过氧化物酶体增殖激活受体β/δ在非酒精性脂肪肝中的作用  |  Zarei, M., et al. 2021. Metabolism. 114: 154342. PMID: 32810487
  6. 过氧化物酶体增殖激活受体（PPARs）的差异表达：成年大鼠 PPAR-α, -beta 和-gamma 的组织分布。  |  Braissant, O., et al. 1996. Endocrinology. 137: 354-66. PMID: 8536636
  7. 过氧化物酶体增殖激活受体α基因的表达受到压力的刺激，并遵循昼夜节律。  |  Lemberger, T., et al. 1996. J Biol Chem. 271: 1764-9. PMID: 8576180
  8. 多种 PPAR 异构体对脂肪生成的不同激活作用。  |  Brun, RP., et al. 1996. Genes Dev. 10: 974-84. PMID: 8608944
  9. 大鼠肝脏中过氧化物酶体增殖激活受体-α mRNA 的调控。  |  Sterchele, PF., et al. 1996. Arch Biochem Biophys. 326: 281-9. PMID: 8611035
  10. 孤儿核荷尔蒙受体 LXR alpha 与过氧化物酶体增殖激活受体相互作用，抑制过氧化物酶体增殖因子信号传导。  |  Miyata, KS., et al. 1996. J Biol Chem. 271: 9189-92. PMID: 8621574
  11. 甲状腺激素和过氧化物酶体增殖物激活受体在调节过氧化物酶体增殖物反应基因中的相互关系  |  Hunter, J., et al. 1996. Mol Cell Endocrinol. 116: 213-21. PMID: 8647322
  12. 过氧化物酶体增殖激活受体（PPAR）在小鼠结肠粘膜中的表达。  |  Mansén, A., et al. 1996. Biochem Biophys Res Commun. 222: 844-51. PMID: 8651933

订购信息

产品名称产品编号规格价格数量收藏夹

PPARβ 抗体 (F-10)

sc-74517
200 µg/ml
$316.00

PPARβ (F-10): m-IgG Fc BP-HRP 套装

sc-527035
200 µg Ab; 10 µg BP
$354.00

PPARβ (F-10): m-IgG1 BP-HRP 套装

sc-532408
200 µg Ab; 20 µg BP
$354.00

PPARβ 抗体 (F-10) X

sc-74517 X
200 µg/0.1 ml
$316.00

PPARβ 抗体 (F-10) AC

sc-74517 AC
500 µg/ml, 25% agarose
$416.00

PPARβ 抗体 (F-10) HRP

sc-74517 HRP
200 µg/ml
$316.00

PPARβ 抗体 (F-10) FITC

sc-74517 FITC
200 µg/ml
$330.00

PPARβ 抗体 (F-10) PE

sc-74517 PE
200 µg/ml
$343.00

PPARβ 抗体 (F-10) Alexa Fluor® 488

sc-74517 AF488
200 µg/ml
$357.00

PPARβ 抗体 (F-10) Alexa Fluor® 546

sc-74517 AF546
200 µg/ml
$357.00

PPARβ 抗体 (F-10) Alexa Fluor® 594

sc-74517 AF594
200 µg/ml
$357.00

PPARβ 抗体 (F-10) Alexa Fluor® 647

sc-74517 AF647
200 µg/ml
$357.00

PPARβ 抗体 (F-10) Alexa Fluor® 680

sc-74517 AF680
200 µg/ml
$357.00

PPARβ 抗体 (F-10) Alexa Fluor® 790

sc-74517 AF790
200 µg/ml
$357.00

I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 13
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with PPARβ (F-10): sc-74517?

Asked by: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-12
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Rated 4 out of 5 by from Good antibodyNice antibody. It detected specific band with mouse liver tissue at 1:500 dilution.
Date published: 2021-12-03
Rated 1 out of 5 by from da ritestarenon sono convinta. Mi riprometto di ritestare l'anticorpo PPARβ cambiando condizioni e tipi cellulari
Date published: 2020-11-18
Rated 2 out of 5 by from good antibodywe tested this antibody in wb in dilution 1/250 in Hela cells. the bands were detected were faint.
Date published: 2019-04-01
Rated 5 out of 5 by from Great antibodyI have used this antibody for protein extracts from breast cancer cell lines and it works really well
Date published: 2018-07-31
Rated 5 out of 5 by from Great!I used this antobody in different mouse breast cancer cell lines and got clear bands with the expected molecular weight.
Date published: 2017-12-12
Rated 5 out of 5 by from The antibody works well.I bought the antibody a month ago, and I am happy that it did work very well.
Date published: 2017-11-26
Rated 5 out of 5 by from The effect is very good.PPARβ Antibody is used to detect immunofluorescence, the signal specificity is very strong, the signal is also strong.
Date published: 2017-10-25
Rated 5 out of 5 by from This antibody works wellPPARβ Antibody are amazing. It proves helpful and efficient and give us a pretty impression in whole cell lysates, the results was satisfactory.
Date published: 2017-10-22
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PPARβ 抗体 (F-10) is rated 4.6 out of 5 by 22.
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