Date published: 2025-9-6
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PP2A-B55-α Antibody (2G9) - Western Blotting - Image 19154
PP2A-B55α (2G9): sc-81606. NRK (A), KNRK (B), 804G (C), A-10 (D) 和 PC-12 (E) 全细胞裂解液及大鼠肝组织提取物 (F) 中 PP2A-B55α 表达的 Western 印迹分析.
PP2A-B55α (2G9): sc-81606. NRK (A), KNRK (B), 804G (C), A-10 (D) 和 PC-12 (E) 全细胞裂解液及大鼠肝组织提取物 (F) 中 PP2A-B55α 表达的 Western 印迹分析.

PP2A-B55-α 抗体 (2G9): sc-81606

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说明书
  • PP2A-B55-alpha 抗体 2G9 是小鼠单克隆 IgG1 κ，PP2A-B55-α抗体, 在23篇文献中引用，规格为200 µg/ml
  • 免疫rat物种的PP2A-B55-α的氨基酸398-411
  • PP2A-B55-alpha 抗体 (2G9) 推荐用于WB, IP 和 IF， 检测 mouse, rat, human, chicken 和 frog (Xenopus laevis) 来源的 PP2A-B55α; 也和以下物种反应，包括： 和 rabbit and porcine; 还建议用于检测PP2A-B55δ of rat origin; 可能与 PP2A-B55β of human origin and weakly with PP2A-B55γ of rabbit origin产生交联反应
  • 抗PP2A-B55-alpha抗体（2G9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PP2A-B55-α (2G9): sc-81606的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是PP2A-B55-α Antibody (2G9) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与PP2A-B55-α Antibody (2G9) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

附加PP2抗体，包括PP2A-A α, PP2A-A alpha / β, PP2A-B55, PP2A-B55-α, PP2A-B55-γ, PP2A-B56-α, PP2A-B56-δ, PP2A-B56-γ, PP2A-C, PP2A-C alpha / β, PP2B-A α, PP2B-A β, PP2B-A γ, PP2B-B1, PP2B-B2和PP2C α

PP2A-B55-α抗体（2G9）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测小鼠、大鼠、人类和鸡源的PP2A-B55-α蛋白。抗PP2A-B55-alpha抗体（2G9）有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。PP2A-B55-alpha蛋白通过调节各种底物的磷酸化状态在细胞信号传导中发挥关键作用，而磷酸化状态对于细胞分裂、细胞凋亡和维持细胞稳态等过程至关重要。PP2A-B55-alpha蛋白主要位于细胞质和细胞核中，与催化亚基和可变亚基形成三聚体复合物，从而发挥有效的调节功能。PP2A-B55与特定底物的相互作用对于调节信号通路至关重要，失调与包括癌症在内的多种疾病有关。了解PP2A-B55-alpha的定位和功能对于阐明细胞过程的作用和潜在的治疗靶点非常重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PP2A-B55-α 抗体 (2G9) 参考文献:

  1. 蛋白磷酸酶 2A 的新型调控亚基 B delta 的克隆与特征。  |  Strack, S., et al. 1999. FEBS Lett. 460: 462-6. PMID: 10556517
  2. 小鼠钙调蛋白依赖性蛋白磷酸酶调节亚基（钙调蛋白 B）两种异构体的结构和表达。  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
  3. Ppp2r2a 基因敲除小鼠揭示了蛋白磷酸酶 2A 调控亚基 PP2A-B55α 是神经元和表皮胚胎发育的重要调控因子。  |  Panicker, N., et al. 2020. Front Cell Dev Biol. 8: 358. PMID: 32582689
  4. PP2A调节亚基PPP2R2A控制NAD+的生物合成，从而调节系统性自身免疫中T细胞亚群的分化。  |  Pan, W., et al. 2024. Cell Rep. 43: 114379. PMID: 38889006
  5. 低 PPP2R2A 表达可提高高级别浆液性卵巢癌对 CHK1 抑制剂的敏感性。  |  Qiu, Z., et al. 2024. Theranostics. 14: 7450-7469. PMID: 39659585
  6. 蛋白磷酸酶-1 的核抑制剂 NIPP-1 的分子克隆揭示了其与参与 RNA 处理的多肽的同源性。  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
  7. 丝氨酸/苏氨酸蛋白磷酸酶。  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
  8. 新型蛋白磷酸酶对丝氨酸和苏氨酸残基去磷酸化的重要作用  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
  9. 平滑肌肌球蛋白结合磷酸酶的调节亚基。  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
  10. 蛋白磷酸酶 2A 72 kDa 调节亚基的结构和表达。替代剪接产生不同大小形式的证据。  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
  11. 蛋白丝氨酸/苏氨酸磷酸酶：结构, 调节和在细胞生长中的功能。  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923
  12. 脑蛋白磷酸酶 2A：B 亚基对发育的调控以及不同的细胞和亚细胞定位。  |  Strack, S., et al. 1998. J Comp Neurol. 392: 515-27. PMID: 9514514

订购信息

产品名称产品编号规格价格数量收藏夹

PP2A-B55-α 抗体 (2G9)

sc-81606
200 µg/ml
$316.00

PP2A-B55-α (2G9): m-IgG1 BP-HRP 套装

sc-531651
200 µg Ab; 20 µg BP
$354.00

PP2A-B55-α 抗体 (2G9) AC

sc-81606 AC
500 µg/ml, 25% agarose
$416.00

PP2A-B55-α 抗体 (2G9) HRP

sc-81606 HRP
200 µg/ml
$316.00

PP2A-B55-α 抗体 (2G9) FITC

sc-81606 FITC
200 µg/ml
$330.00

PP2A-B55-α 抗体 (2G9) PE

sc-81606 PE
200 µg/ml
$343.00

PP2A-B55-α 抗体 (2G9) Alexa Fluor® 488

sc-81606 AF488
200 µg/ml
$357.00

PP2A-B55-α 抗体 (2G9) Alexa Fluor® 546

sc-81606 AF546
200 µg/ml
$357.00

PP2A-B55-α 抗体 (2G9) Alexa Fluor® 594

sc-81606 AF594
200 µg/ml
$357.00

PP2A-B55-α 抗体 (2G9) Alexa Fluor® 647

sc-81606 AF647
200 µg/ml
$357.00

PP2A-B55-α 抗体 (2G9) Alexa Fluor® 680

sc-81606 AF680
200 µg/ml
$357.00

PP2A-B55-α 抗体 (2G9) Alexa Fluor® 790

sc-81606 AF790
200 µg/ml
$357.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-13

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with PP2A-B55-α (2G9): sc-81606?

Asked by: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-13

What is this antibody diluted in? I am trying to label this antibody with biotin and I need to know if there are some components not compatable to it?

Asked by: divya
Thank you for your question. This antibody vial contains 200 µg IgG1 in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2016-11-08
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Rated 5 out of 5 by from Outstanding !!Very nice western blot data with low background; strong IF nuclear staining pattern
Date published: 2017-01-20
Rated 5 out of 5 by from Strong pull down in IP in human UStrong pull down in IP in human U-2 OS, glioblastoma T98G cells. -SCBT Publication Review
Date published: 2015-05-17
Rated 5 out of 5 by from Produced exceptional immunofluorescence nuclearProduced exceptional immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-16
Rated 5 out of 5 by from Published for IP and WB in mouse brainPublished for IP and WB in mouse brain. -SCBT Publication Review
Date published: 2015-01-15
Rated 5 out of 5 by from Single strong band in WB in NRKSingle strong band in WB in NRK, KNRK, 804G, A-10 and PC-12 whole cell lysates and rat liver tissue extract. -SCBT QC
Date published: 2015-01-02
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PP2A-B55-α 抗体 (2G9) is rated 5.0 out of 5 by 5.
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