Date published: 2025-9-6
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Polycystin-1 Antibody (7E12) - Western Blotting - Image 67392
Polycystin-1 抗体 (7E12): sc-130554. 纯化的人类尿液外泌体 (A) 和纯化的 MDCK 膜 (B) 中 Polycystin-1 表达的 Western 印迹分析.
Polycystin-1 Antibody (7E12) - Western Blotting - Image 67392
Polycystin-1 抗体 (7E12): sc-130554. 纯化的人类尿液外泌体 (A) 和纯化的 MDCK 膜 (B) 中 Polycystin-1 表达的 Western 印迹分析.
Polycystin-1 Antibody (7E12) - Western Blotting - Image 355277
Polycystin-1 抗体 (7E12): sc-130554. WI-38 全细胞裂解液中 Polycystin-1 表达的 Western 印迹分析.
Polycystin-1 Antibody (7E12) - Western Blotting - Image 384692
Polycystin-1 Antibody (7E12): sc-130554. Western 印迹分析 EOC 20 全细胞裂解液中 Polycystin-1 的表达.
Polycystin-1 抗体 (7E12): sc-130554. 纯化的人类尿液外泌体 (A) 和纯化的 MDCK 膜 (B) 中 Polycystin-1 表达的 Western 印迹分析.

Polycystin-1 抗体 (7E12): sc-130554

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说明书
  • Polycystin-1 抗体 7E12 是小鼠单克隆 IgG1 κ，Polycystin-1抗体, 在63篇文献中引用，规格为200 µg/ml
  • 针对与human起源的Polycystin-1的N-terminal亮氨酸富集域对应的氨基酸24-180
  • Polycystin-1 抗体 (7E12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Polycystin-1
  • Polycystin-1 抗体 (7E12) 可偶联 琼脂糖 用于 IP; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Polycystin-1 (7E12): sc-130554的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Polycystin-1 Antibody (7E12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Polycystin-1 Antibody (7E12) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Polycystin抗体，包括Polycystin-1和Polycystin-2

Polycystin-1抗体（7E12）是一种IgG1 κ型小鼠单克隆Polycystin-1抗体（也被指定为PKD1抗体或PC1抗体），可通过WB, IP, IF和ELISA检测小鼠、大鼠和人源的Polycystin-1蛋白。Polycystin-1抗体（7E12）既可以非共轭抗Polycystin-1抗体的形式提供，也可以多种共轭形式的抗Polycystin-1抗体提供，包括琼脂糖、PE、FITC和多种Alexa Fluor®共轭物。常染色体显性遗传性多囊肾病（ADPKD）的特征是在肾小管以及肝脏和胰腺组织中形成囊肿。这些囊肿内的细胞在增殖和极性方面显示出异常。在大多数ADPKD患者中，整体膜蛋白Polycystin-1（PKD1）发生突变。Polycystin-1在肾小管上皮细胞中表达，并与细胞和局灶性粘附蛋白（包括E-钙粘蛋白、连环蛋白、纽蛋白和桩蛋白）共定位到局灶区域，以形成更大的多蛋白复合物。Polycystin-1经酪氨酸磷酸化翻译后修饰，并与Polycystin-2（PKD2）结合以介导AP-1表达，这表明Polycystin-1参与细胞-细胞和细胞-基质相互作用以控制细胞增殖和极性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Polycystin-1 抗体 (7E12) 参考文献:

  1. 常染色体显性多囊肾病基因产物 PKD2 引发的细胞活化。  |  Arnould, T., et al. 1999. Mol Cell Biol. 19: 3423-34. PMID: 10207066
  2. 常染色体显性多囊肾病蛋白--多囊卵巢蛋白-2 和多囊卵巢蛋白-1 在正常组织和囊肿组织中的协调表达。  |  Ong, AC., et al. 1999. Am J Pathol. 154: 1721-9. PMID: 10362797
  3. PKD1, 早发 PKD1 和 TSC2/PKD1 囊性组织中多囊卵巢素-1 的表达。  |  Ong, AC., et al. 1999. Kidney Int. 56: 1324-33. PMID: 10504485
  4. PKD1 基因的产物 '多囊卵巢蛋白-1 '是一种酪氨酸磷酸化蛋白，它与 alpha2beta1-integrin 共同定位在附着的肾上皮细胞的局灶簇中。  |  Wilson, PD., et al. 1999. Lab Invest. 79: 1311-23. PMID: 10532593
  5. Polycystin-1是PKD1基因的产物，与E-cadherin和catenins组成复合物。  |  Huan, Y. and van Adelsberg, J. 1999. J Clin Invest. 104: 1459-68. PMID: 10562308
  6. 新型肾脏多囊卵巢素-1-多囊卵巢素-2 复合物的鉴定, 表征和定域。  |  Newby, LJ., et al. 2002. J Biol Chem. 277: 20763-73. PMID: 11901144
  7. ADPKD 的遗传机制。  |  Kim, DY. and Park, JH. 2016. Adv Exp Med Biol. 933: 13-22. PMID: 27730431
  8. 多囊卵巢蛋白-1调节心肌细胞的有丝分裂。  |  Ramírez-Sagredo, A., et al. 2021. FASEB J. 35: e21796. PMID: 34324238
  9. 多囊卵胞素-1的C终止子以线粒体酶依赖的方式抑制囊肿病。  |  Onuchic, L., et al. 2023. Nat Commun. 14: 1790. PMID: 36997516
  10. 机械敏感蛋白多囊蛋白-1能促进骨折愈合过程中骨膜干细胞/祖细胞的骨软骨分化。  |  Liu, R., et al. 2024. Theranostics. 14: 2544-2559. PMID: 38646641
  11. 机械蛋白聚胞素-1 直接调节破骨细胞生成和骨吸收。  |  Huang, M., et al. 2024. Sci Bull (Beijing). 69: 1964-1979. PMID: 38760248
  12. 多囊肾病 1 基因产物介导蛋白激酶 C alpha 依赖性和 c-Jun N 端激酶依赖性激活转录因子 AP-1。  |  Arnould, T., et al. 1998. J Biol Chem. 273: 6013-8. PMID: 9497315

订购信息

产品名称产品编号规格价格数量收藏夹

Polycystin-1 抗体 (7E12)

sc-130554
200 µg/ml
$316.00

Polycystin-1 (7E12): m-IgG Fc BP-HRP 套装

sc-528735
200 µg Ab; 10 µg BP
$354.00

Polycystin-1 (7E12): m-IgGκ BP-HRP 套装

sc-521215
200 µg Ab, 40 µg BP
$354.00

Polycystin-1 (7E12): m-IgG1 BP-HRP 套装

sc-543103
200 µg Ab; 20 µg BP
$354.00

Polycystin-1 抗体 (7E12) AC

sc-130554 AC
500 µg/ml, 25% agarose
$416.00

Polycystin-1 抗体 (7E12) FITC

sc-130554 FITC
200 µg/ml
$330.00

Polycystin-1 抗体 (7E12) PE

sc-130554 PE
200 µg/ml
$343.00

Polycystin-1 抗体 (7E12) Alexa Fluor® 488

sc-130554 AF488
200 µg/ml
$357.00

Polycystin-1 抗体 (7E12) Alexa Fluor® 546

sc-130554 AF546
200 µg/ml
$357.00

Polycystin-1 抗体 (7E12) Alexa Fluor® 594

sc-130554 AF594
200 µg/ml
$357.00

Polycystin-1 抗体 (7E12) Alexa Fluor® 647

sc-130554 AF647
200 µg/ml
$357.00

Polycystin-1 抗体 (7E12) Alexa Fluor® 680

sc-130554 AF680
200 µg/ml
$357.00

Polycystin-1 抗体 (7E12) Alexa Fluor® 790

sc-130554 AF790
200 µg/ml
$357.00

What is the antibody dilution recommended for western blotting using 30 ug of total cell lysates?

Asked by: XXXXXX
Thank you for your question. We recommend a starting of 1:100-1:000 . https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2024-03-28

What size in kDa do you expect this antibody to run on a Western Blot for mouse kidney?

Asked by: Abc3456
Thank you for your question. 485 kDa
Answered by: BlakeJ
Date published: 2023-08-11

What's the gel percentage used for this polycystin-1 antibody in WB? Also, is there any suitable reference protein or markers?

Asked by: yybio
Thank you for your question. We used a 4-6% gel for this very large protein. These will be very fragile, so handle carefully. Please contact our Technical Service Department for suggestions of suitable high molecular weight proteins that could be used as a reference or molecular weight marker.
Answered by: Technical Support
Date published: 2019-08-17

Can you provide with a FACS protocol for this antibody (polycystin-1, 7E12) on cell lines?

Asked by: Curious2
Thank you for your question. We do not have a specific FACS protocol for use with this antibody. Please check our general protocols online, or contact our Technical Support for more information.
Answered by: Technical Support
Date published: 2019-04-13

I recently ordered this mAb conjugated to PE. I'm attempting to use this mAb to quantitate a cell surface antigen - to do that I need to know the ratio of PE molecules per mAb. I.E., do you know if there is one PE bound to each mAb, or two, etc?

Asked by: Jeff R
Thank you for your question. For PE conjugated antibodies, the ratio is 1:1, so there is 1 PE per antibody molecule.
Answered by: Tech Service
Date published: 2019-03-25

Which part does this antibody (polycystin-1, 7E12) recognize?

Asked by: yybio
Thank you for your question. This monoclonal antibody was raised against amino acids 24-180 corresponding to the N-terminal leucine rich domain of Polycystin-1 of human origin. We will update our product description for future reference.
Answered by: Technical Support
Date published: 2018-12-10

Is it possible to use the AC conjugated Polycystin-1 Antibody (7E12): sc-130554 AC in both Western Blot and IP?

Asked by: jenni
Thank you for your inquiry. We do not recommend the use of the AC conjugated form in Western blot. The AC conjugated form of the antibody is specifically used for IP. The unconjugated form of the antibody is the best choice when working in multiple applications.
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 5 out of 5 by from suggest this antibody to you.Great antibody! It can detect pKD1 protein in HELA and HEK.
Date published: 2022-10-14
Rated 5 out of 5 by from WB功能很好1月前购买此产品，货期较短，发货较快，WB效果好，希望可以得到更多的应用。其他应用没有涉及，后期使用再做评论。
Date published: 2022-02-07
Rated 5 out of 5 by from Good for your Western blot!An excellent detection reagent for your WB. If you need to visualize polycystin-1 in human samples, this antibody can do it. Provides a nice clear band and has been cited over ten times.
Date published: 2017-01-16
Rated 4 out of 5 by from Strong westernVery nice western blot data; strong signal with low background
Date published: 2017-01-06
Rated 5 out of 5 by from Used this antibody in WB and got good resultsUsed this antibody in WB and got good results.
Date published: 2015-08-17
Rated 5 out of 5 by from Successful application of this antibody inSuccessful application of this antibody in WB using human PDL fibroblasts -SCBT Publication Review
Date published: 2015-04-19
Rated 5 out of 5 by from WB publishable data with mIMCD cellsWB publishable data with mIMCD cells - SCBT Publication Review
Date published: 2015-03-30
Rated 5 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for WB on human HEK293 cells - SCBT Publication Review
Date published: 2015-03-18
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Polycystin-1 抗体 (7E12) is rated 4.9 out of 5 by 8.
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