Date published: 2025-9-7

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PMCA 抗体 (F-3): sc-271917

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说明书
  • PMCA 抗体 F-3 是小鼠单克隆 IgG1 κ,PMCA抗体, 在4篇文献中引用,规格为200 µg/ml
  • 针对氨基酸481-780,在human起源的PMCA的细胞质域内进行映射
  • PMCA 抗体 (F-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 PMCA1, PMCA2, PMCA3 and PMCA4
  • 抗PMCA抗体(F-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PMCA (F-3): sc-271917免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 PMCA 抗体 (F-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PMCA 抗体 (F-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

PMCA抗体(F-3)是一种IgG1κ小鼠单克隆PMCA抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的PMCA蛋白。PMCA抗体(F-3)既可作为非缀合的抗PMCA抗体形式,也可作为多种缀合形式的抗PMCA抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®缀合物。质膜型Ca2+-ATP酶(PMCAs)负责将二价钙离子从真核细胞中排出。作为P类离子运动ATP酶的成员,PMCAs是由至少四个不同基因的交替剪接转录本衍生而来的一组功能多样的蛋白质。不同PMCA同工型和剪接变体的表达受到发育、组织和细胞类型特异性的调控,并且与特定细胞和组织类型的生理需求有关。真核细胞中静息细胞内Ca2+浓度的时空变化速率和Ca2+信号传导依赖于与Ca2+排出速率相协调表达的PMCA同工型阵列。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PMCA 抗体 (F-3) 参考文献:

  1. 星形胶质细胞中的质膜钙 ATP 酶同工酶  |  Fresu, L., et al. 1999. Glia. 28: 150-5. PMID: 10533058
  2. 替代剪接在产生质膜钙泵同工酶多样性中的作用。  |  Strehler, EE. and Zacharias, DA. 2001. Physiol Rev. 81: 21-50. PMID: 11152753
  3. Ca2+ 突然增加对质膜钙泵的延迟激活:快速泵存在于快速细胞中。  |  Caride, AJ., et al. 2001. Cell Calcium. 30: 49-57. PMID: 11396987
  4. 血小板活化过程中,质膜 Ca(2+)-ATP 酶(PMCA)易位到丝状体。  |  Dean, WL. and Whiteheart, SW. 2004. Thromb Haemost. 91: 325-33. PMID: 14961161
  5. 编码质膜 Ca2(+)-transporting ATPases 的两个基因在人类染色体 1q25-32 和 12q21-23 上的定域。  |  Olson, S., et al. 1991. Genomics. 9: 629-41. PMID: 1674727
  6. 糖尿病患者血小板 PMCA 和 SERCA 型 Ca2+ -ATPase 的表达:巨核细胞生成异常的新特征。  |  Chaabane, C., et al. 2007. J Thromb Haemost. 5: 2127-35. PMID: 17883705
  7. 在 T 细胞活化过程中,STIM1 是减弱 PMCA 介导的 Ca2+ 清除所必需的。  |  Ritchie, MF., et al. 2012. EMBO J. 31: 1123-33. PMID: 22246182
  8. 通过将 Glu99 突变为 Lys 使人类质膜 Ca2+ 泵 PMCA h4xb 超活化。  |  Mazzitelli, LR. and Adamo, HP. 2014. J Biol Chem. 289: 10761-10768. PMID: 24584935
  9. 分子克隆了主要在大脑和骨骼肌中表达的钙调素敏感性质膜 Ca2+ 转运 ATP 酶的第三种同工酶。  |  Greeb, J. and Shull, GE. 1989. J Biol Chem. 264: 18569-76. PMID: 2530223
  10. 通过 PMCA 检测甲醛固定的 MSA 患者组织中的α-突触核蛋白播种活性。  |  Becker, K., et al. 2018. Mol Neurobiol. 55: 8728-8737. PMID: 29589283
  11. Ca2+ 输出泵 PMCA 清除近膜 Ca2+ 以促进储存操作的 Ca2+ 进入和 NFAT 激活。  |  Go, CK., et al. 2019. Sci Signal. 12: PMID: 31594854
  12. PMCA 用于证明可重复使用医疗器械上朊病毒灭活方法的有效性:动物生物测定的相关替代方法。  |  Igel, A., et al. 2024. J Hosp Infect. 154: 60-63. PMID: 39357541

订购信息

产品名称产品编号规格价格数量收藏夹

PMCA 抗体 (F-3)

sc-271917
200 µg/ml
$316.00

PMCA (F-3): m-IgG Fc BP-HRP 套装

sc-529286
200 µg Ab; 10 µg BP
$354.00

PMCA (F-3): m-IgGκ BP-HRP 套装

sc-522100
200 µg Ab, 40 µg BP
$354.00

PMCA (F-3): m-IgG1 BP-HRP 套装

sc-543432
200 µg Ab; 20 µg BP
$354.00

PMCA 抗体 (F-3) AC

sc-271917 AC
500 µg/ml, 25% agarose
$416.00

PMCA 抗体 (F-3) HRP

sc-271917 HRP
200 µg/ml
$316.00

PMCA 抗体 (F-3) FITC

sc-271917 FITC
200 µg/ml
$330.00

PMCA 抗体 (F-3) PE

sc-271917 PE
200 µg/ml
$343.00

PMCA 抗体 (F-3) Alexa Fluor® 488

sc-271917 AF488
200 µg/ml
$357.00

PMCA 抗体 (F-3) Alexa Fluor® 546

sc-271917 AF546
200 µg/ml
$357.00

PMCA 抗体 (F-3) Alexa Fluor® 594

sc-271917 AF594
200 µg/ml
$357.00

PMCA 抗体 (F-3) Alexa Fluor® 647

sc-271917 AF647
200 µg/ml
$357.00

PMCA 抗体 (F-3) Alexa Fluor® 680

sc-271917 AF680
200 µg/ml
$357.00

PMCA 抗体 (F-3) Alexa Fluor® 790

sc-271917 AF790
200 µg/ml
$357.00

I am using PMCA (F-3): sc-271917 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Performed well in WB in NIH/3T3 whole cell lysatePerformed well in WB in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using K-562 whole cell lysate. -SCBT QC
Date published: 2015-02-24
Rated 5 out of 5 by from Superb immunofluorescence cytoplasmic stainingSuperb immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-21
Rated 5 out of 5 by from Robust signal observed in WB in mouse brainRobust signal observed in WB in mouse brain, rat cerebellum and human hippocampus tissue extracts. -SCBT QC
Date published: 2015-02-13
Rated 5 out of 5 by from Band detected at expected mw in HeLaBand detected at expected mw in HeLa, Ramos and KNRK whole cell lysates. -SCBT QC
Date published: 2015-01-25
Rated 5 out of 5 by from Performs well in WesternPerforms well in Western, good signal with IMR-32 whole cell lysate. -SCBT QC
Date published: 2015-01-07
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PMCA 抗体 (F-3) is rated 5.0 out of 5 by 6.
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