Date published: 2025-9-6
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PLZF Antibody (D-9) - Western Blotting - Image 361842
PLZF 抗体 (D-9): sc-28319 HRP. TF-1 (A) 和 MEG-01 (B) 全细胞裂解液中 PLZF 表达的直接 Western 印迹分析.
PLZF 抗体 (D-9): sc-28319 HRP. TF-1 (A) 和 MEG-01 (B) 全细胞裂解液中 PLZF 表达的直接 Western 印迹分析.

PLZF 抗体 (D-9): sc-28319

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说明书
  • PLZF 抗体 D-9 是小鼠单克隆 IgG1 κ，PLZF抗体, 在189篇文献中引用，规格为200 µg/ml
  • 免疫human物种的PLZF的氨基酸101-400
  • PLZF 抗体 (D-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PLZF
  • 抗PLZF抗体（D-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PLZF (D-9): sc-28319的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 PLZF 抗体 (D-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PLZF 抗体 (D-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

PLZF抗体（D-9）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的PLZF蛋白。抗PLZF抗体（D-9）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。PLZF蛋白（也称为ZBTB16或ZNF145）在转录调控中起着至关重要的作用，并参与各种细胞类型的发展，尤其是免疫系统。PLZF的结构特征是五个锌指结构域和一个宽复合物POZ（或BTB）结构域，它们对于转录因子的功能至关重要。PLZF BTB/POZ结构域促进蛋白质-蛋白质相互作用，允许与其他转录调节因子形成复合物，从而影响基因表达。PLZF与肿瘤抑制基因的调控有关，失调与多种癌症有关，包括急性早幼粒细胞白血病，其中PLZF与视黄酸受体α（RARA）形成融合蛋白，导致转录活性改变。了解PLZF的结构特征和相互作用对于阐明其在细胞过程中的作用以及作为癌症治疗潜在治疗靶点至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PLZF 抗体 (D-9) 参考文献:

  1. 广义复合体, tramtrack 和 bric à brac/poxviruses 的进化分化以及癌症中高甲基化候选肿瘤抑制基因的锌指结构域。  |  Guerardel, C., et al. 1999. FEBS Lett. 451: 253-6. PMID: 10371200
  2. SMRT/N-CoR-mSin3A-HDAC-抑制复合物的招募不是 BTB/POZ 转录抑制因子的一般机制：HIC-1 和 gammaFBP-B 的案例。  |  Deltour, S., et al. 1999. Proc Natl Acad Sci U S A. 96: 14831-6. PMID: 10611298
  3. TIMP-1 通过与 PLZF 的第九锌指结构域相互作用来调节细胞增殖。  |  Rho, SB., et al. 2007. J Cell Biochem. 101: 57-67. PMID: 17340613
  4. 人类内源性逆转录病毒蛋白 Np9 和 rec 与早幼粒细胞白血病锌指蛋白的物理和功能相互作用。  |  Denne, M., et al. 2007. J Virol. 81: 5607-16. PMID: 17360752
  5. PLZF作为前列腺癌中抑癌基因的作用。  |  Jin, Y., et al. 2017. Oncotarget. 8: 71317-71324. PMID: 29050363
  6. PLZF限制肠道防御中的肠道ILC3功能。  |  Xu, Y., et al. 2023. Cell Mol Immunol. 20: 379-388. PMID: 36693920
  7. PLZF蛋白与蛋白TET1形成复合物，靶向未分化的精原细胞中的TCF7L2。  |  Du, X., et al. 2024. Theriogenology. 215: 321-333. PMID: 38128225
  8. p53 可激活 17p13.3 上新的候选肿瘤抑制基因 HIC-1 的表达。  |  Wales, MM., et al. 1995. Nat Med. 1: 570-7. PMID: 7585125
  9. 与 mSin3A 相关的组蛋白去乙酰化酶介导了急性早幼粒细胞白血病相关 PLZF 蛋白的抑制作用。  |  David, G., et al. 1998. Oncogene. 16: 2549-56. PMID: 9627120
  10. SMRT核心压制复合体的成分与POZ结构域肿瘤蛋白PLZF, PLZF-RARalpha和BCL-6表现出独特的相互作用。  |  Wong, CW. and Privalsky, ML. 1998. J Biol Chem. 273: 27695-702. PMID: 9765306
  11. PLZF BTB 结构域的晶体结构。  |  Ahmad, KF., et al. 1998. Proc Natl Acad Sci U S A. 95: 12123-8. PMID: 9770450
  12. BCL-6 POZ 结构域和其他 POZ 结构域与共抑制因子 N-CoR 和 SMRT 相互作用。  |  Huynh, KD. and Bardwell, VJ. 1998. Oncogene. 17: 2473-84. PMID: 9824158

订购信息

产品名称产品编号规格价格数量收藏夹

PLZF 抗体 (D-9)

sc-28319
200 µg/ml
$316.00

PLZF (D-9): m-IgG Fc BP-HRP 套装

sc-526661
200 µg Ab; 10 µg BP
$354.00

PLZF (D-9): m-IgG1 BP-HRP 套装

sc-532034
200 µg Ab; 20 µg BP
$354.00

PLZF 抗体 (D-9) AC

sc-28319 AC
500 µg/ml, 25% agarose
$416.00

PLZF 抗体 (D-9) HRP

sc-28319 HRP
200 µg/ml
$316.00

PLZF 抗体 (D-9) FITC

sc-28319 FITC
200 µg/ml
$330.00

PLZF 抗体 (D-9) PE

sc-28319 PE
200 µg/ml
$343.00

PLZF 抗体 (D-9) Alexa Fluor® 488

sc-28319 AF488
200 µg/ml
$357.00

PLZF 抗体 (D-9) Alexa Fluor® 546

sc-28319 AF546
200 µg/ml
$357.00

PLZF 抗体 (D-9) Alexa Fluor® 594

sc-28319 AF594
200 µg/ml
$357.00

PLZF 抗体 (D-9) Alexa Fluor® 647

sc-28319 AF647
200 µg/ml
$357.00

PLZF 抗体 (D-9) Alexa Fluor® 680

sc-28319 AF680
200 µg/ml
$357.00

PLZF 抗体 (D-9) Alexa Fluor® 790

sc-28319 AF790
200 µg/ml
$357.00

I am using this antibody to do immunofluorescence staining of mouse testes and I can't get a positive signal no matter what method I use, is there a special procedure for this antibody?

Asked by: bbzz
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2024-05-31

Can this ZBTB16(D-9) antibody use in CHIP assay?

Asked by: jnwek123
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2023-05-21

Is this antibody can be used for Flow cytometry. If yes then what is the dilution. Do you also have a small pack of this antibody.

Asked by: ashish79
Thank you for your question. Yes, we are aware of several publications citing use of this PLZF Antibody (D-9): sc-28319 in flow cytometry (FCM). For example, see PMID: 22473038. We recommend using 1 µg of fluorochrome-conjugated antibodies in 100 µl of the prepared cell suspension (equal to 1 million cells) to each tube. A link to our recommended protocol is below as a reference. https://www.scbt.com/scbt/resources/protocols/flow-cytometry We are currently offering a free 10 µg sample size of the unconjugated primary antibody. Please contact our Technical Service Department if you would like to request a free sample. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-08-21

Dear Sir/Madam, I am planning to do ChIP experiment for PLZF protein. So I am wondering is it possible to get the same antibody in more concentrated form (200ug/0.1ml) like you have fo other antibodies. Thanks, Waiting for your reply, Sathish

Asked by: Satsa
Thank you for your question. The more concentrated X-form (200 µg/0.1 ml) of this PLZF Antibody (D-9): sc-28319 is available as a special order. Please contact our Special Orders Department to inquire about the details. Please call our European Support office toll-free at 00800 4573 8000, or e-mail specialorders@scbt.com.
Answered by: Technical Support
Date published: 2017-03-20

What is the recommended detection reagent for this mouse primary antibody since SCBT no longer sells traditional secondary antibodies?

Asked by: DefinitelyNotMatt
Thank you for your question. As an alternative to conventional secondary antibodies, use our great Mouse Binding Proteins! They are recombinant proteins that detect and bind to mouse antibodies; they come conjugated to HRP for WBs as well as many other conjugates for uses in different applications. https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-29
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Rated 4 out of 5 by from nice for immunofluorescent stainingI stained mouse testis frozen sections with this antibody and it works when the tissue section was pretreated with citrate buffer.
Date published: 2019-11-07
Rated 5 out of 5 by from 抗体很好用我半个月前购买了此产品，效果非常好，无杂带，条带准确，非常完美，已经推荐其他实验室老师采购该产品！！！
Date published: 2017-12-23
Rated 5 out of 5 by from good resultPLZF antibody was diluted with 1:200 in adrenal gland tissues of human and stained with IHC, strong staining and excellent image.
Date published: 2017-11-01
Rated 3 out of 5 by from PLZF antibody on mouse testesI got this antibody as a courtesy from SCBT to try PLZF protein on moue testis spermatogonia by using IHC. Antigen retrieval 12 min in Na citrate. Permeabilization 30 min 0.2%Triton X-100/PBS. Blocking peroxidase 30 min. Primary 1:200 ON. Secondary 1:200 1h.
Date published: 2017-10-12
Rated 5 out of 5 by from Renin modulates HIV replication in T cellsrenin affects HIV -associated nephropathy. This antibody was very useful and provided great information for the publication.
Date published: 2017-07-25
Rated 5 out of 5 by from This antibody's effect on WB is very good .This antibody very cost-effective ,next time will continue to buy.
Date published: 2017-04-26
Rated 5 out of 5 by from Great features!clean and got the right binds for WB. validated by KO
Date published: 2017-04-11
Rated 5 out of 5 by from Good descripitionThe clear result got with cervical carcinoma cell PLZF by WB at 1:1000 dilution.
Date published: 2017-03-28
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PLZF 抗体 (D-9) is rated 4.8 out of 5 by 20.
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