Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Plk Double Nickase Plasmid (m): sc-422309-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Plk Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Plk Double Nickase Plasmid (m) and Plk Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Plk1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Plk Antibody (F-8): sc-17783
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Plk Double Nickase Plasmid (m) | sc-422309-NIC | 20 µg | $410.00 | |||
Plk Double Nickase Plasmid (m2) | sc-422309-NIC-2 | 20 µg | $410.00 |
Mouse Plk1 encodes polo-like kinase 1 (Plk), a serine/threonine kinase that coordinates key transitions through mitosis by regulating centrosome maturation, bipolar spindle assembly, chromosome segregation, and cytokinesis. Plk1 activity integrates with CDK1–cyclin B signaling and controls multiple mitotic substrates, including proteins involved in kinetochore function and microtubule dynamics, to ensure accurate cell-cycle progression. Dysregulated Plk1 expression or activity is linked to aberrant proliferation and genome instability, making it a widely used node for studying mitotic checkpoints, chromosomal instability, and cell fate decisions in dividing mammalian cells.
Plk Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Plk1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Plk1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Plk1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Plk1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.