PKR Double Nickase Plasmid (rabbit): sc-437324-NIC
- Target species: rabbit
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PKR Double Nickase Plasmid (rabbit) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PKR Double Nickase Plasmid (rabbit) and PKR Double Nickase Plasmid (rabbit2) encode distinct paired gRNA designs targeting . One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKR Double Nickase Plasmid (rabbit) | sc-437324-NIC | 20 µg | $410.00 | |||
PKR Double Nickase Plasmid (rabbit2) | sc-437324-NIC-2 | 20 µg | $410.00 |
PKR (EIF2AK2) is a double-stranded RNA–activated serine/threonine kinase that functions as a key sensor of viral and endogenous RNA stress, linking innate immune detection to translational control. Upon activation, PKR phosphorylates eIF2α to suppress global protein synthesis and modulates signaling through NF-κB, MAPK, and interferon-stimulated gene networks, shaping antiviral and inflammatory responses. PKR also contributes to stress granule dynamics, apoptosis, and regulation of cytokine production, positioning it at the interface of proteostasis and immune signaling. Dysregulated PKR activity has been associated with chronic inflammation, metabolic dysfunction, and neurodegeneration, making it a relevant node for mechanistic studies in rabbit models and primary cell systems.
PKR Double Nickase Plasmid (rabbit) consists of a matched pair of plasmids engineered for high-specificity editing of the locus in rabbit cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within . When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of -disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.