PITHD1 CRISPR/Cas9 KO Plasmid (h): sc-412754
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PITHD1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PITHD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PITHD1 Antibody (A-10): sc-515392
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PITHD1 CRISPR/Cas9 KO Plasmid (h) | sc-412754 | 20 µg | $397.00 |
Overview
PITHD1 encodes a small, conserved protein implicated in proteostasis through interactions with the 26S proteasome, linking it to regulated protein turnover and cellular stress adaptation. By modulating proteasome-associated processes, PITHD1 can influence the stability of short-lived signaling and cell-cycle regulators that shape proliferation and survival phenotypes. Altered expression of PITHD1 has been reported in multi-omics studies across several tumor contexts, supporting its use as a research target for dissecting ubiquitin–proteasome system dependencies. Functional interrogation of PITHD1 is therefore relevant to pathways governing protein quality control, stress responses, and transcriptome/proteome remodeling.
PITHD1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PITHD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PITHD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PITHD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PITHD1 protein expression.
This CRISPR knockout system enables efficient generation of PITHD1-deficient cell models for investigation of PITHD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PITHD1 exon(s) critical for PITHD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PITHD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PITHD1 CRISPR/Cas9 KO Plasmid (h) and PITHD1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PITHD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PITHD1 HDR Plasmid (h) and PITHD1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PITHD1 homology arms to support homology-directed repair at defined PITHD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.