Date published: 2025-9-7
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PI 3-kinase p110γ Antibody (D-12) - Western Blotting - Image 398056
PI 3-kinase p110γ 抗体 (D-12): sc-166365. K-562 (A), RAW 264. 7 (B) 和 RBL-1 (C) 全细胞裂解液中 PI 3- 激酶 p110γ 表达的 Western 印迹分析. 所用检测试剂: m-IgG1 BP-HRP: sc-525408.
PI 3-kinase p110γ 抗体 (D-12): sc-166365. K-562 (A), RAW 264. 7 (B) 和 RBL-1 (C) 全细胞裂解液中 PI 3- 激酶 p110γ 表达的 Western 印迹分析. 所用检测试剂: m-IgG1 BP-HRP: sc-525408.

PI 3-kinase p110γ 抗体 (D-12): sc-166365

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寫評論提問

说明书
  • PI 3-kinase p110 gamma 抗体 D-12 是小鼠单克隆 IgG1 κ，PI 3-kinase p110γ抗体, 在16篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的PI 3-kinase p110γ的N-terminus的氨基酸32-58之间
  • PI 3-kinase p110 gamma 抗体 (D-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PI 3-kinase p110γ
  • 抗PI 3-kinase p110 gamma抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PI 3-kinase p110γ (D-12): sc-166365的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 PI 3-kinase p110γ 抗体 (D-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PI 3-kinase p110γ 抗体 (D-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 PI 3-kinase 抗体包括 PI 3-kinase C2 α, PI 3-kinase C2 β, PI 3-kinase C2 γ, PI 3-kinase p100, PI 3-kinase p101, PI 3-kinase p110, PI 3-kinase p110 α, PI 3-kinase p110 β, PI 3-kinase p110 δ 和 PI 3-kinase p110 γ

PI 3-激酶p110γ抗体（D-12）是一种IgG1 κ小鼠单克隆PI 3-激酶p110 gamma抗体（也称为PI3K抗体、p110 gamma抗体、PIK3CG抗体或磷脂酰肌醇-4,5-二磷酸3-激酶催化亚基gamma抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的PI 3-激酶p110 gamma蛋白。PI 3-激酶p110γ抗体（D-12）既可作为非偶联抗PI 3-激酶p110 gamma抗体形式，也可作为多种偶联形式的抗PI 3-激酶p110 gamma抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。磷脂酰肌醇3-激酶（PI 3-激酶）由p85和p110亚基组成。p85缺乏PI 3-激酶活性，并作为适配器，将p110与活化的蛋白酪氨酸激酶偶联。已经描述了两种形式的p85（p85α和p85β），每种都具有一个SH3和两个SH2结构域。已经鉴定出各种p110异构体。p110α和p110β与p85α相互作用，并且已经显示p110α在体外与p85β相互作用。p110δ表达仅限于白细胞。已经显示它与p85α和β结合，但它显然不磷酸化这些亚基。p110δ似乎具有自磷酸化的能力。p110γ不与p85亚基相互作用。已经显示它被α和βγ异三聚体G蛋白激活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PI 3-kinase p110γ 抗体 (D-12) 参考文献:

  1. 磷脂酰肌醇 3-激酶：110 kd 催化亚基的结构和表达。  |  Hiles, ID., et al. 1992. Cell. 70: 419-29. PMID: 1322797
  2. 与受体酪氨酸激酶, middle-T/pp60c-src 复合物和 PI3 激酶缔合作用的两种 85 kd 蛋白的特征。  |  Otsu, M., et al. 1991. Cell. 65: 91-104. PMID: 1707345
  3. 利用表达/克隆受体酪氨酸激酶靶蛋白的新方法克隆 PI3 激酶缔合作用 p85。  |  Skolnik, EY., et al. 1991. Cell. 65: 83-90. PMID: 1849461
  4. 克隆 G 蛋白激活的人类磷酸肌醇-3 激酶并确定其特性。  |  Stoyanov, B., et al. 1995. Science. 269: 690-3. PMID: 7624799
  5. 克隆一种新的, 普遍表达的人类磷脂酰肌醇 3- 激酶，并确定其在 p85 上的结合位点。  |  Hu, P., et al. 1993. Mol Cell Biol. 13: 7677-88. PMID: 8246984
  6. 白细胞中的新型磷酸肌醇 3- 激酶 P110delta  |  Vanhaesebroeck, B., et al. 1997. Proc Natl Acad Sci U S A. 94: 4330-5. PMID: 9113989

订购信息

产品名称产品编号规格价格数量收藏夹

PI 3-kinase p110γ 抗体 (D-12)

sc-166365
200 µg/ml
$316.00

PI 3-kinase p110γ (D-12): m-IgG Fc BP-HRP 套装

sc-527157
200 µg Ab; 10 µg BP
$354.00

PI 3-kinase p110γ (D-12): m-IgG1 BP-HRP 套装

sc-532530
200 µg Ab; 20 µg BP
$354.00

PI 3-kinase p110γ 抗体 (D-12) AC

sc-166365 AC
500 µg/ml, 25% agarose
$416.00

PI 3-kinase p110γ 抗体 (D-12) HRP

sc-166365 HRP
200 µg/ml
$316.00

PI 3-kinase p110γ 抗体 (D-12) FITC

sc-166365 FITC
200 µg/ml
$330.00

PI 3-kinase p110γ 抗体 (D-12) PE

sc-166365 PE
200 µg/ml
$343.00

PI 3-kinase p110γ 抗体 (D-12) Alexa Fluor® 488

sc-166365 AF488
200 µg/ml
$357.00

PI 3-kinase p110γ 抗体 (D-12) Alexa Fluor® 546

sc-166365 AF546
200 µg/ml
$357.00

PI 3-kinase p110γ 抗体 (D-12) Alexa Fluor® 594

sc-166365 AF594
200 µg/ml
$357.00

PI 3-kinase p110γ 抗体 (D-12) Alexa Fluor® 647

sc-166365 AF647
200 µg/ml
$357.00

PI 3-kinase p110γ 抗体 (D-12) Alexa Fluor® 680

sc-166365 AF680
200 µg/ml
$357.00

PI 3-kinase p110γ 抗体 (D-12) Alexa Fluor® 790

sc-166365 AF790
200 µg/ml
$357.00

PI 3-kinase p110γ (D-12) 中和勝肽

sc-166365 P
100 µg/0.5 ml
$68.00

怎样获取sc-166365的免费使用装小样？

Asked by: Anonymous
感谢您的提问。如何获取免费试用装，请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。 Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 9
Date published: 2019-05-05

What application is the blocking peptide sc-166365 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 效果很不错之前订购了该产品，收到后使用下来很不错。用过其他品牌的抗体，santa的算好的。或给身边的其他人推荐。
Date published: 2022-07-11
Rated 1 out of 5 by from I couldn't detect a bandI loaded 80ug of protein, 1:250 dilution in 5% milk-TBST, 1:1000 of secondary and I was not able to see anything. I tried different lysates and nothing.
Date published: 2017-11-28
Rated 5 out of 5 by from Great antibodyA satisfactory result was obtained for a specific strong band when I used in human bone marrow tissue with 1:500 diluted.
Date published: 2017-10-25
Rated 5 out of 5 by from Effect is Quite Good.PI 3-kinase p110γ Antibody is very vivid, fierceness and stable.The staining intensity between these cells was obvious, it is highly suitable to show cellular staining.
Date published: 2017-10-18
Rated 4 out of 5 by from Good for controlsWe used this antibody for detecting mouse p110γ by western blot (dilution 1:1000) and the band was clear and specific.
Date published: 2017-09-14
Rated 5 out of 5 by from Good antibodyWe have used this antibody for detecting human p110γ by western blot (dilution 1:500) and the band was strong and clear. Highly recommended.
Date published: 2017-07-25
Rated 5 out of 5 by from GOODGood WB antibody in human pancreatic cancer cell line PANC1.
Date published: 2017-06-30
Rated 5 out of 5 by from The effect is very good.Staining data very strong,the effect is very good.
Date published: 2017-04-28
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PI 3-kinase p110γ 抗体 (D-12) is rated 4.6 out of 5 by 14.
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