Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PI 3-kinase p110α Double Nickase Plasmid (h): sc-416642-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PI 3-kinase p110α Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PI 3-kinase p110α Double Nickase Plasmid (h) and PI 3-kinase p110α Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PIK3CA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PI 3-kinase p110α Antibody (E-7): sc-518070
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PI 3-kinase p110α Double Nickase Plasmid (h) | sc-416642-NIC | 20 µg | $410.00 | |||
PI 3-kinase p110α Double Nickase Plasmid (h2) | sc-416642-NIC-2 | 20 µg | $410.00 |
PIK3CA encodes the catalytic p110α subunit of class I phosphoinositide 3-kinase (PI3K), which phosphorylates PIP2 to generate PIP3 at the plasma membrane. This lipid signaling event recruits and activates AKT and downstream effectors including mTOR, coordinating growth factor–driven control of proliferation, metabolism, survival, and cytoskeletal dynamics. PI3K/AKT/mTOR signaling interfaces with receptor tyrosine kinases, RAS, and feedback loops that shape cellular stress responses and nutrient sensing. Altered PIK3CA activity is frequently studied in contexts of dysregulated signaling networks underlying oncogenic transformation, altered glucose utilization, and aberrant cell migration.
PI 3-kinase p110α Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PIK3CA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PIK3CA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PIK3CA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PIK3CA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.