Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PI 3-kinase p100 CRISPR/Cas9 KO Plasmid (m): sc-432531
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PI 3-kinase p100 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PI 3-kinase p100 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PI 3-kinase p100 Antibody (F-11): sc-365404
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PI 3-kinase p100 CRISPR/Cas9 KO Plasmid (m) | sc-432531 | 20 µg | $397.00 |
Overview
Pik3c3 encodes the class III phosphatidylinositol 3-kinase catalytic subunit (often referred to as PI 3-kinase p100/Vps34), which generates phosphatidylinositol 3-phosphate to control endosomal membrane identity and trafficking. In mouse cells, PI3P production by this kinase is central to autophagy initiation, endosome-to-lysosome maturation, and vesicular sorting through complexes with regulatory partners such as VPS15 and Beclin 1. Pik3c3 activity integrates nutrient and stress cues to coordinate lysosomal homeostasis and cellular quality control, influencing processes including proteostasis, mitochondrial turnover, and immune signaling. Dysregulated endolysosomal and autophagy pathways linked to Pik3c3 function are frequently studied in the context of neurodegeneration, infection biology, and tumor cell metabolism.
PI 3-kinase p100 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pik3c3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pik3c3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pik3c3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PI 3-kinase p100 protein expression.
This CRISPR knockout system enables efficient generation of Pik3c3-deficient cell models for investigation of PI 3-kinase p100 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pik3c3 exon(s) critical for PI 3-kinase p100 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pik3c3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PI 3-kinase p100 CRISPR/Cas9 KO Plasmid (m) and PI 3-kinase p100 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pik3c3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PI 3-kinase p100 HDR Plasmid (m) and PI 3-kinase p100 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pik3c3 homology arms to support homology-directed repair at defined Pik3c3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.