Date published: 2025-9-6
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PI 3-kinase C2α Antibody (G-5) - Western Blotting - Image 360338
PI 3-kinase C2α (G-5): sc-365290. HeLa (A), PC-3 (B) 和 HEK293 (C) 全细胞裂解液中 PI 3-kinase C2α 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
PI 3-kinase C2α (G-5): sc-365290. HeLa (A), PC-3 (B) 和 HEK293 (C) 全细胞裂解液中 PI 3-kinase C2α 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

PI 3-kinase C2α 抗体 (G-5): sc-365290

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说明书
  • PI 3-kinase C2 alpha 抗体 G-5 是小鼠单克隆 IgG1 κ，PI 3-kinase C2α抗体, 在35篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列61-360位于human物种的PI 3-kinase C2α的N-terminus附近
  • PI 3-kinase C2 alpha 抗体 (G-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 PI 3-kinase C2α
  • 抗PI 3-kinase C2 alpha抗体（G-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PI 3-kinase C2α (G-5): sc-365290的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是PI 3-kinase C2α Antibody (G-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PI 3-kinase C2α Antibody (G-5) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加PI 3-kinase抗体，包括PI 3-kinase C2 α, PI 3-kinase C2 β, PI 3-kinase C2 γ, PI 3-kinase p100, PI 3-kinase p101, PI 3-kinase p110, PI 3-kinase p110 α, PI 3-kinase p110 β, PI 3-kinase p110 δ, PI 3-kinase p110 γ, PI 3-kinase p55 γ, PI 3-kinase p85 α和PI 3-kinase p85 β

PI 3-激酶C2α抗体（G-5）是一种IgG1 κ小鼠单克隆PI 3-激酶C2 alpha抗体（也被称为PI3K C2 alpha抗体、PIK3C2A抗体或磷脂酰肌醇-4-磷酸3-激酶催化亚基2 alpha抗体），它能通过WB、IP、IF和ELISA等方法检测小鼠、大鼠和人类来源的PI 3-激酶C2 alpha蛋白。PI 3-激酶C2α抗体（G-5）既有非偶联形式的抗PI 3-激酶C2 alpha抗体，也有多种偶联形式的抗PI 3-激酶C2 alpha抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。磷脂酰肌醇3-激酶（PI3Ks）可磷酸化肌醇脂的肌醇环的3' OH位置。人类PI 3-激酶C2a（PIK3C2A，含C2的磷脂酰肌醇激酶，p110a或CPK）含有一个C端钙结合和磷脂结合模块，即C2结构域。PI 3-激酶C2a的cDNA序列预测产生一个含1686个氨基酸的蛋白质，该蛋白质与小鼠Cpk（果蝇同源物的术语）有90%的相似性。Northern印迹分析表明，PI 3-激酶C2a在各种组织中均表达为8 kb的mRNA。在体外，PI 3-激酶C2a酶可以磷酸化磷脂酰肌醇和磷脂酰肌醇-4-磷酸。PI 3-激酶C2a基因包含32个外显子，跨越约76 kb。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PI 3-kinase C2α 抗体 (G-5) 参考文献:

  1. 绘制人类染色体 11p15.1-->p14 上基因丰富的 400-kb 区域的基因和转录序列图。  |  Caldwell, GM., et al. 2001. Cytogenet Cell Genet. 92: 103-7. PMID: 11306805
  2. [磷脂酰肌醇-3-激酶 P85/P110 和 hVPS34 在表皮生长因子受体复合物的内吞过程中的作用]。  |  Zheleznova, NN., et al. 2003. Tsitologiia. 45: 574-81. PMID: 14521088
  3. 调节胰岛素敏感组织中磷酸肌醇 3- 激酶信号的机制。  |  Shepherd, PR. 2005. Acta Physiol Scand. 183: 3-12. PMID: 15654916
  4. 抑制 II 类磷酸肌醇 3- 激酶基因的α-异构体表达会导致细胞凋亡。  |  Kang, S., et al. 2005. Biochem Biophys Res Commun. 329: 6-10. PMID: 15721265
  5. 在培养的狗气道平滑肌细胞中，胰蛋白酶可独立于蛋白酶激活受体-2以蛋白水解方式激活磷脂酰肌醇-3-激酶。  |  Brown, JK., et al. 2006. Am J Physiol Lung Cell Mol Physiol. 290: L259-69. PMID: 16155087
  6. 磷酸肌醇 3 激酶 C2alpha 结构域的单个活性可独立调节凝集素的功能。  |  Gaidarov, I., et al. 2005. J Biol Chem. 280: 40766-72. PMID: 16215232
  7. 突变型 p110alpha 和 p110beta 磷脂肌醇 3- 激酶在人类乳腺上皮细胞中的致癌特性。  |  Zhao, JJ., et al. 2005. Proc Natl Acad Sci U S A. 102: 18443-8. PMID: 16339315
  8. 磷脂酰肌醇 3- 激酶作为生长和新陈代谢调节剂的演变。  |  Engelman, JA., et al. 2006. Nat Rev Genet. 7: 606-19. PMID: 16847462
  9. 磷酸肌醇 3 激酶 C2alpha 将凝集素与微管依赖性运动联系起来。  |  Zhao, Y., et al. 2007. J Biol Chem. 282: 1249-56. PMID: 17110375
  10. 磷酸肌酸 3-激酶-C2&α；调节多囊卵巢素-2 的纤毛进入并防止肾囊肿形成  |  Franco, I., et al. 2016. J Am Soc Nephrol. 27: 1135-44. PMID: 26271513
  11. Cpk 是一类新型果蝇 PtdIns 3 激酶，含有一个 C2 结构域。  |  Molz, L., et al. 1996. J Biol Chem. 271: 13892-9. PMID: 8662856
  12. 克隆具有 C2 结构域的人磷酸肌醇 3- 激酶，该酶对抑制剂沃特曼素的敏感性降低。  |  Domin, J., et al. 1997. Biochem J. 326 (Pt 1): 139-47. PMID: 9337861

订购信息

产品名称产品编号规格价格数量收藏夹

PI 3-kinase C2α 抗体 (G-5)

sc-365290
200 µg/ml
$316.00

PI 3-kinase C2α (G-5): m-IgG Fc BP-HRP 套装

sc-529343
200 µg Ab; 10 µg BP
$354.00

PI 3-kinase C2α (G-5): m-IgGκ BP-HRP 套装

sc-522192
200 µg Ab, 40 µg BP
$354.00

PI 3-kinase C2α (G-5): m-IgG1 BP-HRP 套装

sc-543468
200 µg Ab; 20 µg BP
$354.00

PI 3-kinase C2α 抗体 (G-5) AC

sc-365290 AC
500 µg/ml, 25% agarose
$416.00

PI 3-kinase C2α 抗体 (G-5) HRP

sc-365290 HRP
200 µg/ml
$316.00

PI 3-kinase C2α 抗体 (G-5) FITC

sc-365290 FITC
200 µg/ml
$330.00

PI 3-kinase C2α 抗体 (G-5) PE

sc-365290 PE
200 µg/ml
$343.00

PI 3-kinase C2α 抗体 (G-5) Alexa Fluor® 488

sc-365290 AF488
200 µg/ml
$357.00

PI 3-kinase C2α 抗体 (G-5) Alexa Fluor® 546

sc-365290 AF546
200 µg/ml
$357.00

PI 3-kinase C2α 抗体 (G-5) Alexa Fluor® 594

sc-365290 AF594
200 µg/ml
$357.00

PI 3-kinase C2α 抗体 (G-5) Alexa Fluor® 647

sc-365290 AF647
200 µg/ml
$357.00

PI 3-kinase C2α 抗体 (G-5) Alexa Fluor® 680

sc-365290 AF680
200 µg/ml
$357.00

PI 3-kinase C2α 抗体 (G-5) Alexa Fluor® 790

sc-365290 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with PI 3-kinase C2α (G-5): sc-365290 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibody for wbThis antibody detects PI3K C2 alpha at the expected molecular weight.
Date published: 2022-06-27
Rated 5 out of 5 by from Works well for WBWorked well at 1:200 dilution when probing mouse tissue lysates.
Date published: 2018-03-23
Rated 5 out of 5 by from SalThis antibody is good for western blot have tried with whole cell lysate from Jurkat T cells
Date published: 2017-10-17
Rated 4 out of 5 by from great featureI bought this antibody a month ago and I am very glad to say this antibody is very good.
Date published: 2017-09-27
Rated 5 out of 5 by from works well for WBIt works well for western blot in lung cancer cell
Date published: 2017-08-02
Rated 4 out of 5 by from Works well for Western blottingHave used this antibody on Western blots and it works well.
Date published: 2017-07-21
Rated 5 out of 5 by from Good antibodyI am happy I purchase this antibody. It was very stable and the result could be repeated.I will continue to use it.
Date published: 2017-02-13
Rated 5 out of 5 by from perform well in the liver tissue for WBthis product can present clear bands when use it in western blot. BTW, my workmate use it in IP, also it worked.
Date published: 2017-01-13
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PI 3-kinase C2α 抗体 (G-5) is rated 4.8 out of 5 by 10.
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