Phosphate Buffered Saline: powder for 1 L of 10X

用法 :

• Reconstitute powder in ddH20, bringing final volume to 1 L (10X). Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 10 L of 1X PBS buffer with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. The PBS solution pH will be approximately 7.4.



• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).


• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.

物理状态 :

Solid

pH :

When diluted to a 1X concentration, the pH will be ~7.4.

保存 :

Store at room temperature