Phosphate Buffered Saline: 100 ml of 10X A 10X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research.

Phosphate Buffered Saline: 100 ml of 10X

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同义词: 10X PBS
应用; A 10X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research
* 参考分析证明 大量特定数据 (包括水 含量).
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Phosphate Buffered Saline: 100 ml of 10X is a concentrated buffer solution ready for dilution to a 1X Phosphate-buffered saline (PBS) working solution commonly used in biological research. PBS buffer is ideal for maintaining a constant pH, and since it is isotonic and non-toxic to cells, it can be used in a wide variety of biological applications. PBS is frequently used as a wash and dilution buffer, and is also used to take a reference spectrum when measuring protein adsorption.
Additional sizes, formats, and concentrations available:
Phosphate Buffered Saline: 1 L of 1X (sc-362182)
Phosphate Buffered Saline: 500 ml of 10X (sc-362298)
Phosphate Buffered Saline: 1 L of 10X (sc-24946)
Phosphate Buffered Saline: 1 L of 20X (sc-362183)
Phosphate Buffered Saline: 5 L of 20X (sc-362299)
Phosphate Buffered Saline: powder for 100 ml of 10X (sc-362300)
Phosphate Buffered Saline: powder for 500 ml of 10X (sc-362301)
Phosphate Buffered Saline: powder for 1 L of 10X (sc-362302)
Phosphate Buffered Saline: powder for 5 L of 10X (sc-24947)


参考文献

1. Dulbecco, R. and Vogt, M. 1954. J. Exp. Med. 99: 167-182. PMID: 13130792
2. Wilcox, C.S., et al. 1992. Proc. Natl. Acad. Sci. U.S.A. 89: 11993-11997. PMID: 1281548
3. Fernando, P.N., et al. 2001. J Chromatogr A. 920: 155-162. PMID: 11452994
4. Kang, J., et al. 2009. Anal. Biochem. 386: 251-255. PMID: 19150324
5. Andersson, J. and Dahlgren, U. 2010. J Dent Res. PMID: 20811070
6. Sambrook, et al. 1989. Rnai: Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press. New York. Cold Spring Harbor.Vol. 3.

用法 :
• Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 1 L of 1X PBS buffer with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. The PBS solution pH will be approximately 7.4.



• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).


• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.
pH :
When diluted to a 1X concentration, the pH will be ~7.4.
保存 :
Store at room temperature
仅供科研使用。不可用于诊断或治疗。

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PMID: # 23946635  Joachim, SC.|Reinehr, S.|Kuehn, S.|Laspas, P.|Gramlich, OW.|Kuehn, M.|Tischoff, I.|von Pein, HD.|Dick, HB.|Grus, FH.| et al. 2013. Mol. Vis. 19: 1804-14.

PMID: # 21907703  Enunlu, I. et al. 2011. Biochem. Biophys. Res. Commun. 413: 471-475.

PMID: # 12631081  Moe, SM. et al. 2003. Kidney international. 63: 1003-11.

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