Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Peroxin 16 Double Nickase Plasmid (h): sc-411278-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Peroxin 16 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Peroxin 16 Double Nickase Plasmid (h) and Peroxin 16 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PEX16. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Peroxin 16 Antibody (H-4): sc-398189
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Peroxin 16 Double Nickase Plasmid (h) | sc-411278-NIC | 20 µg | $410.00 | |||
Peroxin 16 Double Nickase Plasmid (h2) | sc-411278-NIC-2 | 20 µg | $410.00 |
PEX16 encodes peroxin 16, a peroxisomal membrane protein that functions early in peroxisome biogenesis by coordinating membrane assembly and the recruitment of additional peroxins required for organelle formation. Peroxin 16 supports establishment and maintenance of the peroxisomal compartment, enabling key lipid metabolic pathways such as very-long-chain fatty acid β-oxidation and ether phospholipid (plasmalogen) synthesis, as well as peroxisome-dependent redox homeostasis. Disruption of PEX16 impairs peroxisome formation and downstream metabolic capacity, linking its dysfunction to peroxisome biogenesis disorders with broad cellular consequences. Because peroxisomal defects influence lipid signaling, oxidative stress responses, and organelle crosstalk, PEX16 is a relevant target for mechanistic studies in metabolic and neurodevelopmental disease models.
Peroxin 16 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PEX16 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PEX16. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PEX16 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PEX16-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.