Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PEPT1 Double Nickase Plasmid (h): sc-401259-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PEPT1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PEPT1 Double Nickase Plasmid (h) and PEPT1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC15A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PEPT1 Antibody (E-3): sc-373742
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PEPT1 Double Nickase Plasmid (h) | sc-401259-NIC | 20 µg | $410.00 | |||
PEPT1 Double Nickase Plasmid (h2) | sc-401259-NIC-2 | 20 µg | $410.00 |
SLC15A1 encodes PEPT1, a proton-coupled oligopeptide transporter that mediates uptake of di- and tripeptides and peptide-like xenobiotics across the apical membrane of epithelial cells, particularly in the small intestine. PEPT1 supports nutrient absorption and cellular nitrogen homeostasis by coupling substrate transport to the transmembrane H+ gradient, integrating with amino acid utilization and epithelial metabolic programs. Its activity influences intestinal barrier physiology and epithelial differentiation, and altered SLC15A1 expression has been reported in inflammatory and metabolic contexts affecting transporter-regulated nutrient handling. Because PEPT1 also transports peptide-mimetic compounds, SLC15A1 serves as a model for studying solute carrier regulation, membrane transport kinetics, and epithelial stress responses.
PEPT1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC15A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC15A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC15A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC15A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.