Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PDK1 CRISPR/Cas9 KO Plasmid (h2): sc-401084-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PDK1 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PDK1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PDK1 Antibody (E-10): sc-515944
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDK1 CRISPR/Cas9 KO Plasmid (h2) | sc-401084-KO-2 | 20 µg | $397.00 |
Overview
Human PDK1 (pyruvate dehydrogenase kinase 1) encodes a mitochondrial kinase that phosphorylates and inhibits the pyruvate dehydrogenase complex, restricting conversion of pyruvate to acetyl-CoA and shifting carbon flux away from the TCA cycle. By modulating glucose oxidation versus lactate production, PDK1 integrates nutrient sensing with hypoxia-responsive metabolic reprogramming and supports adaptation to oxidative stress. Its activity intersects with central metabolic pathways including glycolysis, mitochondrial respiration, and anaplerosis, influencing cellular proliferation and survival decisions. Dysregulated PDK1 expression or activity has been associated with altered bioenergetics observed across multiple disease contexts, making it a relevant target for mechanistic studies of metabolism-driven phenotypes.
PDK1 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the PDK1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PDK1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PDK1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PDK1 protein expression.
This CRISPR knockout system enables efficient generation of PDK1-deficient cell models for investigation of PDK1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PDK1 exon(s) critical for PDK1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PDK1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PDK1 CRISPR/Cas9 KO Plasmid (h) and PDK1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PDK1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PDK1 HDR Plasmid (h) and PDK1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PDK1 homology arms to support homology-directed repair at defined PDK1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.