Date published: 2026-10-16
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PDI Antibody (C-5) - Western Blotting - Image 361053
PDI 抗体 (C-5): sc-376369. 在 HeLa (A), F9 (B), NIH/3T3 (C), C3H/10T1/2 (D), SP2/0 (E) 和 KNRK (F) 全细胞裂解液中 PDI 表达的 Western 印迹分析.
PDI 抗体 (C-5): sc-376369. 在 HeLa (A), F9 (B), NIH/3T3 (C), C3H/10T1/2 (D), SP2/0 (E) 和 KNRK (F) 全细胞裂解液中 PDI 表达的 Western 印迹分析.

PDI 抗体 (C-5): sc-376369

3.6(5)
寫評論提問
说明书
  • PDI抗体(C-5)是小鼠单克隆IgG1 κ, 在2篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的PDI的N-terminus附近的氨基酸97-135之间
  • 推荐用于 mouse, rat 和 human 来源的precursor and mature PDI WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 PDI (C-2): sc-74551，可查看有标记的 PDI 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgGκ BP-HRP是PDI Antibody (C-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与PDI Antibody (C-5) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。
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関連項目

氧化还原酶-蛋白质二硫键异构酶（PDI）是一种由催化硫醇-二硫键交换的亚基组成的同源二聚体，介导新生蛋白质的折叠并作为分子伴侣发挥作用。PDI定位于内质网（ER）腔内，与折叠辅助蛋白（如免疫球蛋白重链结合蛋白BiP）一起介导三级和四级蛋白质加工。细胞表面PDI通过诱导整合素介导的粘附受体-配体相互作用中的硫氢基介导的构象变化，从而调节整合素反应和细胞粘附。此外，PDI还作为另外两个更复杂的酶系统的亚基发挥作用：脯氨酰-4-羟化酶和三酰甘油转移蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PDI 抗体 (C-5) 参考文献:

  1. PDI蛋白家族的结构概述。  |  Kozlov, G., et al. 2010. FEBS J. 277: 3924-36. PMID: 20796029
  2. PorphyChem的一些活动，通过卟啉类化合物在PDT、PIT和PDI中的应用进行说明。  |  Habermeyer, B. and Guilard, R. 2018. Photochem Photobiol Sci. 17: 1675-1690. PMID: 30109347
  3. 基于机理的动能捕获法鉴定PDI底物。  |  Eriksson, O., et al. 2019. Methods Mol Biol. 1967: 165-182. PMID: 31069770
  4. 蛋白质二硫键异构酶（PDI）对低氧诱导因子-1（HIF-1α）表达的调节。  |  Kobayashi, Y., et al. 2021. PLoS One. 16: e0246531. PMID: 33539422
  5. PDI b'xa'结构域构建的主干共振分配。  |  Pei, Y., et al. 2021. Biomol NMR Assign. 15: 409-413. PMID: 34169459
  6. 细胞外Ero1α/PDI电子传输系统通过增加谷胱甘肽还原电位来调节血小板功能。  |  Wang, L., et al. 2022. Redox Biol. 50: 102244. PMID: 35077997
  7. PDI抑制剂LTI6426在多发性骨髓瘤临床前模型中增强了帕诺比司他（panobinostat）的功效。  |  Robinson, RM., et al. 2022. Cancer Chemother Pharmacol. 89: 643-653. PMID: 35381875
  8. 富勒烯-苝二酰亚胺（C60-PDI）系统：锚定工程多功能平台概述与合成。  |  Diacon, A., et al. 2022. Molecules. 27: PMID: 36235059
  9. 设计并合成de novoANTPABA-PDI纳米材料作为环境大气中倒置太阳能电池的受体。  |  Mitra, S., et al. 2023. Nanotechnology. 34: PMID: 37137300
  10. 光亲和标记与蛋白质组学相结合，确定PDI-ADAM17模块是(-)-vinigrol靶向的，可诱导TNFR1脱落并改善小鼠类风湿性关节炎。  |  Zhu, Y., et al. 2024. Cell Chem Biol. 31: 452-464.e10. PMID: 37913771

订购信息

产品名称产品编号规格价格数量收藏夹

PDI 抗体 (C-5)

sc-376369
200 µg/ml
$322.00

PDI (C-5): m-IgGκ BP-HRP 套装

sc-552038
200 µg Ab; 40 µg BP
$361.00

PDI (C-5) 中和勝肽

sc-376369 P
100 µg/0.5 ml
$69.00

What application is the blocking peptide sc-376369 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by citos from Not valid for plant cellsWe have tried different immunofluorescence protocols in Arabidopsis cells and we did not find any distinguishable signals on nuclear envelope.
Date published: 2018-02-05
Rated 4 out of 5 by TS from Good specificity and signal strength withGood specificity and signal strength with some background bands in A-431 whole cell lysate. -SCBT QC
Date published: 2015-03-01
Rated 3 out of 5 by JL from Worked OK with transfected lysate by WB withWorked OK with transfected lysate by WB with some background -SCBT QC
Date published: 2015-01-09
Rated 5 out of 5 by SM from Performs well in WesternPerforms well in Western, good signal with MOLT-4, Raji and A2058 whole cell lysates -SCBT QC
Date published: 2015-01-02
Rated 5 out of 5 by JT from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human prostate tissue. -SCBT QC
Date published: 2013-04-15
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PDI 抗体 (C-5) is rated 3.6 out of 5 by 5.
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