Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
palladin CRISPR/Cas9 KO Plasmid (m2): sc-428317-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- palladin CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the palladin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: palladin Antibody (G-2): sc-166563
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
palladin CRISPR/Cas9 KO Plasmid (m2) | sc-428317-KO-2 | 20 µg | $397.00 |
Overview
Mouse Palld encodes palladin, an actin-associated scaffolding protein that coordinates cytoskeletal organization by binding actin regulators and crosslinking components at stress fibers, focal adhesions, and other adhesion complexes. Palladin supports cell shape control, adhesion maturation, and directed migration, integrating signaling from Rho family GTPases and pathways governing actomyosin contractility and extracellular matrix interactions. Altered palladin expression or localization has been linked to abnormal tissue remodeling and invasive phenotypes in cancer-relevant contexts, making Palld a useful node for studying cytoskeletal dysregulation. In the mouse system, Palld perturbation is commonly used to dissect mechanisms of motility, mechanotransduction, and adhesion-dependent signaling in development and disease models.
palladin CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Palld gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Palld together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Palld open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish palladin protein expression.
This CRISPR knockout system enables efficient generation of Palld-deficient cell models for investigation of palladin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Palld exon(s) critical for palladin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Palld genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by palladin CRISPR/Cas9 KO Plasmid (m) and palladin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Palld locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by palladin HDR Plasmid (m) and palladin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Palld homology arms to support homology-directed repair at defined Palld target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.