Date published: 2025-9-6
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p73 Antibody (E-4) - Western Blotting - Image 27
p73 抗体 (E-4): sc-17823. SK-MEL-24 (A), K-562 (B), HL-60 (C) 和 COS (D) 全细胞裂解液中 p73 表达的 Western 印迹分析.
p73 Antibody (E-4) - Western Blotting - Image 27
p73 抗体 (E-4): sc-17823. SK-MEL-24 (A), K-562 (B), HL-60 (C) 和 COS (D) 全细胞裂解液中 p73 表达的 Western 印迹分析.
p73 Antibody (E-4) - Western Blotting - Image 375151
p73 抗体 (E-4): sc-17823. Western 印迹分析 SJRH30 全细胞裂解液中 p73 的表达.
p73 抗体 (E-4): sc-17823. SK-MEL-24 (A), K-562 (B), HL-60 (C) 和 COS (D) 全细胞裂解液中 p73 表达的 Western 印迹分析.

p73 抗体 (E-4): sc-17823

4.4(11)
寫評論提問

说明书
  • p73抗体(E-4)是小鼠单克隆IgG2a κ, 在24篇文献中引用，规格为200 µg/ml
  • 免疫human来源p73位于N-terminus 的1-80氨基酸
  • 抗-p73 抗体 (E-4) 推荐用于 WB, IP, IF 和 ELISA，检测human 和monkey 来源的 all p73 isoforms
  • 抗p73抗体（E-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p73 (E-4): sc-17823的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是p73 Antibody (E-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与p73 Antibody (E-4) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加p73抗体，包括和ΔN p73

p73抗体（E-4）是一种小鼠单克隆IgG2a kappa轻链抗体，用于检测人类和猴子p73的所有同种型，也称为TP73。p73单克隆抗体（E-4）靶向p73 N端1-80氨基酸内的表位，确保在蛋白质印迹 （WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）中表现出色。p73通过磷酸化和其他翻译后修饰来响应细胞应激信号，包括DNA损伤和癌基因激活，其活性和功能取决于特定的同工型表达和细胞环境，促进细胞周期停滞、细胞凋亡或存活。p73基因位于1号染色体上，该区域在神经母细胞瘤中经常缺失，编码多种异构体，如p73α和p73β，它们对细胞凋亡具有相反的作用。p73α抑制细胞凋亡，而p73β促进细胞凋亡，从而维持防止恶性转化的关键平衡。同种型之间的这种平衡对于细胞调控和癌症预防至关重要。抗p73抗体（E-4）有非结合型和多种结合型，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，为不同的实验设置提供了多功能性。p73（E-4）抗体使研究人员能够有效地研究p73同种型在肿瘤抑制中的复杂作用。 异氰酸酯（FITC）和多种Alexa Fluor®偶联物，为不同的实验设置提供了多功能性。p73 (E-4)抗体使研究人员能够有效地研究p73异构体在肿瘤抑制和细胞凋亡调节中的复杂作用，使其成为癌症研究和分子生物学中不可或缺的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p73 抗体 (E-4) 参考文献:

  1. 酵母双杂交系统显示 p73 alpha 与 SV40 大 T 抗原之间没有相互作用。  |  Reichelt, M., et al. 1999. Arch Virol. 144: 621-6. PMID: 10226625
  2. 凋亡中的p73。  |  Stiewe, T. and Pützer, BM. 2001. Apoptosis. 6: 447-52. PMID: 11595834
  3. 利用 p53 和 GADD45 的哺乳动物细胞周期检查点通路在共济失调-特朗日综合征中存在缺陷。  |  Kastan, MB., et al. 1992. Cell. 71: 587-97. PMID: 1423616
  4. 在抑制 PDGF beta-受体的过程中，p73 与共激活因子竞争，并将组蛋白去乙酰化酶招募到 NF-Y 上。  |  Uramoto, H., et al. 2004. J Cell Sci. 117: 5323-31. PMID: 15454570
  5. 全长 p73alpha 能抑制药物诱导的小细胞肺癌细胞凋亡。  |  Nyman, U., et al. 2005. J Biol Chem. 280: 34159-69. PMID: 16087678
  6. 乳腺癌, 肉瘤和其他肿瘤家族综合征中的生殖系 p53 突变。  |  Malkin, D., et al. 1990. Science. 250: 1233-8. PMID: 1978757
  7. p53：致癌基因还是抗癌基因？  |  Lane, DP. and Benchimol, S. 1990. Genes Dev. 4: 1-8. PMID: 2137806
  8. p73的结构和凋亡功能。  |  Yoon, MK., et al. 2015. BMB Rep. 48: 81-90. PMID: 25441426
  9. Pirh2-AIP4回路对p73的调节。  |  Wu, HH., et al. 2021. Aging (Albany NY). 13: 20858-20859. PMID: 34520394
  10. 与 p53 有关的单平行表达基因，位于 1p36，该区域在神经母细胞瘤和其他人类癌症中经常被删除。  |  Kaghad, M., et al. 1997. Cell. 90: 809-19. PMID: 9288759
  11. p73 是一种能诱导细胞凋亡的与 p53 相关的类人（修正人）蛋白。  |  Jost, CA., et al. 1997. Nature. 389: 191-4. PMID: 9296498
  12. 一种与肿瘤抑制因子 p53 具有很强同源性的新型蛋白质。  |  Schmale, H. and Bamberger, C. 1997. Oncogene. 15: 1363-7. PMID: 9315105

订购信息

产品名称产品编号规格价格数量收藏夹

p73 抗体 (E-4)

sc-17823
200 µg/ml
$316.00

p73 (E-4): m-IgG Fc BP-HRP 套装

sc-525492
200 µg Ab; 10 µg BP
$354.00

p73 (E-4): m-IgG2a BP-HRP 套装

sc-546287
200 µg Ab; 10 µg BP
$354.00

p73 抗体 (E-4) AC

sc-17823 AC
500 µg/ml, 25% agarose
$416.00

p73 抗体 (E-4) HRP

sc-17823 HRP
200 µg/ml
$316.00

p73 抗体 (E-4) FITC

sc-17823 FITC
200 µg/ml
$330.00

p73 抗体 (E-4) PE

sc-17823 PE
200 µg/ml
$343.00

p73 抗体 (E-4) Alexa Fluor® 488

sc-17823 AF488
200 µg/ml
$357.00

p73 抗体 (E-4) Alexa Fluor® 546

sc-17823 AF546
200 µg/ml
$357.00

p73 抗体 (E-4) Alexa Fluor® 594

sc-17823 AF594
200 µg/ml
$357.00

p73 抗体 (E-4) Alexa Fluor® 647

sc-17823 AF647
200 µg/ml
$357.00

p73 抗体 (E-4) Alexa Fluor® 680

sc-17823 AF680
200 µg/ml
$357.00

p73 抗体 (E-4) Alexa Fluor® 790

sc-17823 AF790
200 µg/ml
$357.00

Dear support team. How do you know that this antibody reacts with all p73 isoforms? Did you test this Ab with DN isoform by western? The TA isoform is the first 60a and this antibody is raised against first 80aa. Thank you.

Asked by: Julian Rozenberg
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Does this antibody work for mouse or rat neuron culture at all?

Asked by: Disha
Thank you for your question. We have not tested this antibody for mouse and rat samples, so we can not recommend or warranty this antibody for use on mouse and rat samples. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-03-06

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 5 out of 5 by from good productIt is a good antibody with clean background for WB.
Date published: 2018-09-11
Rated 4 out of 5 by from WB in NG108-15 cellsThis antibody shows a good signal for NG108-15 cells using WB, but the appearance of other faint bands near the band of interest may be a small problem.
Date published: 2018-05-17
Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 4 out of 5 by from Good antibodyThe antibody works great for westernblotting using mouse brain tissue at 1:500 dilution.
Date published: 2017-07-24
Rated 4 out of 5 by from workedOverexpressed HA-p73a, b, g, d (human clone) in 293 cells were detected, respectively. there is no other bands in 293 cells. 1/1000 dil in 2% BSA TBST, o/n reaction.
Date published: 2017-03-24
Rated 5 out of 5 by from Recommended for WBAlthough there was some background, the specific bands were strong and clear.
Date published: 2017-01-11
Rated 5 out of 5 by from Used successfully in IF at 1:50 dilutionUsed successfully in IF at 1:50 dilution.
Date published: 2015-09-23
Rated 5 out of 5 by from Good publishable data in ChIP in mouse andGood publishable data in ChIP in mouse and human H4, MCF7, 293T, Saos2, U2OS cells. -SCBT Publication Review
Date published: 2015-01-21
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p73抗体 (E-4) is rated 4.4 out of 5 by 11.
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