Date published: 2025-9-9
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p70 S6 kinase/S6K1 Antibody (H-9) - Western Blotting - Image 353331
p70 S6 激酶 α 抗体 (H-9): sc-8418. 在 HeLa (A), Jurkat (B), MDA-MB-231 (C), KNRK (D) 和 NIH/3T3 (E) 全细胞裂解液中 p70 S6 激酶 α 表达的 Western 印迹分析.
p70 S6 激酶 α 抗体 (H-9): sc-8418. 在 HeLa (A), Jurkat (B), MDA-MB-231 (C), KNRK (D) 和 NIH/3T3 (E) 全细胞裂解液中 p70 S6 激酶 α 表达的 Western 印迹分析.

p70 S6 kinase/S6K1 抗体 (H-9): sc-8418

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说明书
  • p70 S6 kinase/S6K1 抗体 H-9 是小鼠单克隆 IgG1 κ，p70 S6 kinase/S6K1抗体, 在244篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于rat物种的p70 S6 kinase α的C-terminus的氨基酸429-441之间
  • p70 S6 kinase/S6K1 抗体 (H-9) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 p70 S6 kinase α
  • 抗p70 S6 kinase/S6K1抗体（H-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p70 S6 kinase/S6K1 (H-9): sc-8418的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 p70 S6 kinase/S6K1 抗体 (H-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p70 S6 kinase/S6K1 抗体 (H-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p70 S6 激酶 α 抗体 (H-9) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可特异性识别大鼠来源的 p70 S6 激酶 α C 末端 429-441 氨基酸之间的表位，通过蛋白质印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、免疫组化 化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类样本中的p70 S6激酶α。p70 S6激酶α，也称为S6K1或RPS6KB1，是一种丝氨酸/苏氨酸激酶，通过磷酸化40S核糖体蛋白S6来调节细胞生长、增殖和新陈代谢，从而促进蛋白质合成和细胞增大。p70 S6激酶α位于mTOR（雷帕霉素靶点）信号传导通路下游，整合来自营养物质、生长因子和细胞能量状态的信号，控制细胞周期正常进展所必需的合成代谢和分解代谢过程。p70 S6激酶α的激活涉及由上游激酶（如ERK MAP激酶和PDK1）介导的磷酸化反应，这凸显了其在信号转导通路中的核心作用。p70 S6激酶α活性的失调与多种病理状况有关，包括癌症、肥胖、2型糖尿病和神经退行性疾病，因此它是生物医学研究和治疗开发的重要目标。p70 S6激酶α单克隆抗体 （H-9）有非结合型和多种结合型，包括琼脂糖、HRP、PE、FITC和一系列Alexa Fluor®结合物，为您的研究工作提供了多种实验应用的可能性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p70 S6 kinase/S6K1 抗体 (H-9) 参考文献:

  1. MEK 的主要结构，MEK 是一种蛋白激酶，能使 ERK 基因产物磷酸化。  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  2. ERK和Rsk编码蛋白激酶的核定位和调控。  |  Chen, RH., et al. 1992. Mol Cell Biol. 12: 915-27. PMID: 1545823
  3. 克隆大鼠肝脏中的丝裂原活化 S6 激酶，发现它是第二信使亚家族的一种酶。  |  Kozma, SC., et al. 1990. Proc Natl Acad Sci U S A. 87: 7365-9. PMID: 1699226
  4. 抑制磷脂酰肌醇3-激酶/p70（S6）-激酶途径诱导B16黑色素瘤细胞分化。  |  Minamitani, C., et al. 2010. Mol Cell Endocrinol. 315: 195-200. PMID: 19879324
  5. 参与核糖体蛋白 S6 磷酸化的蛋白激酶的结构, 表达和调节。  |  Erikson, RL. 1991. J Biol Chem. 266: 6007-10. PMID: 2007561
  6. 减数分裂和有丝分裂细胞周期控制中的蛋白激酶级联。  |  Pelech, SL., et al. 1990. Biochem Cell Biol. 68: 1297-330. PMID: 2085430
  7. 主要胰岛素/介素激活的 70-kDa S6 蛋白激酶的分子结构。  |  Banerjee, P., et al. 1990. Proc Natl Acad Sci U S A. 87: 8550-4. PMID: 2236064
  8. 鉴定鸡胚成纤维细胞中的有丝分裂原反应核糖体蛋白 S6 激酶 pp90rsk，它是爪蟾 S6 激酶 II 的同源物。  |  Sweet, LJ., et al. 1990. Mol Cell Biol. 10: 2413-7. PMID: 2325657
  9. 克隆和表达两种人类p70 S6激酶多肽，它们仅在氨基末端不同。  |  Tandelilin, AA., et al. 2015. BMC Endocr Disord. 15: 11. PMID: 25887856
  10. p70 核糖体 S6 激酶调节肺中转化生长因子-α表达后的胸膜下纤维化。  |  Madala, SK., et al. 2016. Am J Physiol Lung Cell Mol Physiol. 310: L175-86. PMID: 26566903
  11. 鸡和小鼠 rsk 的序列和表达：爪蟾核糖体 S6 激酶的同源物。  |  Alcorta, DA., et al. 1989. Mol Cell Biol. 9: 3850-9. PMID: 2779569
  12. AMPD1调节骨骼肌胰岛素敏感性的mTORC1-p70 S6激酶轴。  |  Buscà, R., et al. 1996. J Biol Chem. 271: 31824-30. PMID: 8943224

订购信息

产品名称产品编号规格价格数量收藏夹

p70 S6 kinase/S6K1 抗体 (H-9)

sc-8418
200 µg/ml
$316.00

p70 S6 kinase/S6K1 (H-9): m-IgG Fc BP-HRP 套装

sc-526521
200 µg Ab; 10 µg BP
$354.00

p70 S6 kinase/S6K1 (H-9): m-IgG1 BP-HRP 套装

sc-531894
200 µg Ab; 20 µg BP
$354.00

p70 S6 kinase/S6K1 抗体 (H-9) AC

sc-8418 AC
500 µg/ml, 25% agarose
$416.00

p70 S6 kinase/S6K1 抗体 (H-9) HRP

sc-8418 HRP
200 µg/ml
$316.00

p70 S6 kinase/S6K1 抗体 (H-9) FITC

sc-8418 FITC
200 µg/ml
$330.00

p70 S6 kinase/S6K1 抗体 (H-9) PE

sc-8418 PE
200 µg/ml
$343.00

p70 S6 kinase/S6K1 抗体 (H-9) Alexa Fluor® 488

sc-8418 AF488
200 µg/ml
$357.00

p70 S6 kinase/S6K1 抗体 (H-9) Alexa Fluor® 546

sc-8418 AF546
200 µg/ml
$357.00

p70 S6 kinase/S6K1 抗体 (H-9) Alexa Fluor® 594

sc-8418 AF594
200 µg/ml
$357.00

p70 S6 kinase/S6K1 抗体 (H-9) Alexa Fluor® 647

sc-8418 AF647
200 µg/ml
$357.00

p70 S6 kinase/S6K1 抗体 (H-9) Alexa Fluor® 680

sc-8418 AF680
200 µg/ml
$357.00

p70 S6 kinase/S6K1 抗体 (H-9) Alexa Fluor® 790

sc-8418 AF790
200 µg/ml
$357.00

p70 S6 kinase/S6K1 (H-9) 中和勝肽

sc-8418 P
100 µg/0.5 ml
$68.00

Can I use this mouse monoclonal antibody for immunofluorescence staining of mouse and human cell? And does anyone used this antibody(sc-8418) for immunofluorescence?

Asked by: Ruan
We have a few references validating this antibody for IF on Mouse and Human. To inquire further on the papers please contact Technical Service.
Answered by: Tech Sales
Date published: 2021-07-09

Hi, has anyone used this antibody in some plant tissue and can tell me if it works? Thank.

Asked by: Kenia
Thank you for your question! We do not have any information regarding it's use on plant samples, so we can not say if it will work or not. However, you can get a sample of this antibody so you can try it! Please contact your local distributor if you would like to obtain a sample.
Answered by: Tech Service
Date published: 2017-08-14

Hello, I have to do a WB in Drosophila and I would like to know if anyone has used this antibody (p70 S6 kinase SC8418) before in flies. Thank you very much Ricardo

Asked by: Richy01
Thank you for your question. We are not aware of any publications citing use of this mouse monoclonal antibody, p70 S6 kinase α (H-9): sc-8418, in Drosophila. Based on the epitope, we do not expect that this antibody would be cross-reactive with Drosophila. And, unfortunately, we also do not have any alternative p70 S6 kinase α antibodies that are recommended for use in flies.
Answered by: Technical Support
Date published: 2017-05-10

Saludos, quisiera me aclararan cuál en qué región de la proteína se ubica el epítopo contra el cual fue generado ya que la información no coincide con la de la ficha técnica, gracias

Asked by: Kenia
Hola, gracias por tu pregunta. Este anticuerpo es específico para un mapeo de epítopos entre los aminoácidos 429-441 en el C-Terminus de p70 S6 kinase α origen de rata. Estaremos actualizando la hoja de datos en breve. Hi, Thank you for your question. This antibody is specific for an epitope mapping between amino acids 429-441 at the C-terminus of p70 S6 kinase α of rat origin. We will be updateding the datasheet shortly.
Answered by: Tech Service 3
Date published: 2017-02-15

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-8418 PE), fluorescein (sc-8418 FITC), Alexa Fluor® 488 (sc-8418 AF488) or Alexa Fluor® 647 (sc-8418 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2017-01-04
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Rated 5 out of 5 by from 效果非常令人满意我们使用了p70 S6 kinase/S6K1抗体进行Western Blot实验，效果非常令人满意。该抗体具有高特异性和较强的亲和力，能够精准地检测到p70 S6 kinase的总蛋白，并且背景信号非常低，保证了实验的清晰度。
Date published: 2024-12-19
Rated 4 out of 5 by from Buen resultadoSe obtuvo hace un año y dio buena señal, cabe mencionar que se uso alta concentración de proteina.
Date published: 2024-01-13
Rated 4 out of 5 by from よく検出できますサンプルをいただき、試しました。 イムノブロットを行いましたが、ノイズも少なく、目的の高さにバンドが検出できました。
Date published: 2023-10-20
Rated 5 out of 5 by from total p70 S6 kinase Western blot applicationStrong band detected in mouse crude synaptosomal fraction at the expected molecular weight. Antibody dilution 1:500.
Date published: 2023-04-13
Rated 3 out of 5 by from Okay AntibodyBand was detected but a bit below expected size. Clear nonetheless.
Date published: 2021-12-03
Rated 5 out of 5 by from p70 S6 kinase (H-9)p70 S6 kinase (H-9) antibody was working very well in our WB.
Date published: 2021-07-07
Rated 4 out of 5 by from Good one!This was a replacement for SC393967, which was a bad Ab. SC8418 was much better. It identified a 70-kDa protein although a much higher molecular size signals appeared in some cell extracts.
Date published: 2018-12-14
Rated 5 out of 5 by from Very good antibodyWorks well. I used 30 ug of mouse cell lysates. Blocked using 5% BSA and primary antibody incubation was conducted overnight at 1:200 dilution. Detected a clean band at around 70 kDa.
Date published: 2018-08-15
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p70 S6 kinase/S6K1 抗体 (H-9) is rated 4.5 out of 5 by 48.
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