Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
P5CRL CRISPR/Cas9 KO Plasmid (h): sc-406033
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P5CRL CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the P5CRL genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: P5CRL Antibody (E-63): sc-100487
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P5CRL CRISPR/Cas9 KO Plasmid (h) | sc-406033 | 20 µg | $397.00 |
Overview
PYCRL encodes pyrroline-5-carboxylate reductase–like protein (P5CRL), a member of the NAD(P)H-dependent reductase family implicated in proline and related amino acid redox metabolism. Proline biosynthesis and turnover influence cellular redox balance, NADP(H) homeostasis, and mitochondrial-associated metabolic adaptation, linking PYCRL to broader processes such as oxidative stress responses and biosynthetic flux control. Perturbation of proline pathway enzymes can affect proliferation, extracellular matrix remodeling through collagen-associated demands, and nutrient stress tolerance. Accordingly, PYCRL is of interest for studying metabolic rewiring in disease-relevant contexts where redox and amino acid metabolism are altered, including cancer biology and inherited metabolic dysfunction.
P5CRL CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PYCRL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PYCRL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PYCRL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish P5CRL protein expression.
This CRISPR knockout system enables efficient generation of PYCRL-deficient cell models for investigation of P5CRL signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PYCRL exon(s) critical for P5CRL function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PYCRL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by P5CRL CRISPR/Cas9 KO Plasmid (h) and P5CRL CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PYCRL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by P5CRL HDR Plasmid (h) and P5CRL HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PYCRL homology arms to support homology-directed repair at defined PYCRL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.