Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
p47phox Antibody (D-10) - Western Blotting - Image 380531
p47phox 抗体 (D-10): sc-17845. Ramos (A), Raji (B), NAMALWA (C) 和 U-698-M (D) 全细胞裂解液中 p47phox 表达的 Western 印迹分析.
p47phox 抗体 (D-10): sc-17845. Ramos (A), Raji (B), NAMALWA (C) 和 U-698-M (D) 全细胞裂解液中 p47phox 表达的 Western 印迹分析.

p47phox 抗体 (D-10): sc-17845

4.6(13)
寫評論提問

说明书
  • p47phox 抗体 D-10 是小鼠单克隆 IgG1 κ，p47phox抗体, 在114篇文献中引用，规格为200 µg/ml
  • 免疫human物种的p47-phox的氨基酸196-390
  • p47phox 抗体 (D-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 p47phox
  • 抗p47phox抗体（D-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p47phox (D-10): sc-17845的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是p47phox Antibody (D-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与p47phox Antibody (D-10) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

p47phox抗体（D-10）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测鼠、大鼠和人类来源的p47phox。抗p47phox抗体（D-10）有非结合型和多种结合型 形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。p47phox在免疫反应中起着至关重要的作用，因为p47phox对于激活中性粒细胞和其他吞噬细胞中的NADPH氧化酶复合物至关重要，而该复合物可产生杀死微生物所需的活性氧。p47phox的突变与遗传性慢性肉芽肿病有关，这种疾病会削弱人体抵抗感染的能力。p47phox位于细胞质中，激活后转移到质膜，与细胞色素b558和G蛋白Rac相互作用，形成功能性NADPH氧化酶复合物。这种转移对于酶的活性至关重要，因为这一过程可以组装产生超氧化物所需的组件。此外，p47phox含有两个Src同源3（SH3）结构域，p47phox C-末端SH3结构域已被证明与p67-phox的脯氨酸富集结构域相互作用，表明p47phox可能促进p67-phox向膜的转运，进一步强调了p47phox在免疫反应中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p47phox 抗体 (D-10) 参考文献:

  1. 中性粒细胞 NADPH 氧化酶活化的启动：p47phox 磷酸化和 NOX2 向质膜调动的作用。  |  El-Benna, J., et al. 2008. Semin Immunopathol. 30: 279-89. PMID: 18536919
  2. 中性粒细胞烟酰胺腺嘌呤二核苷酸磷酸氧化酶的组装。p47-phox和p67-phox的转移需要p47-phox和细胞色素b558之间的相互作用。  |  Heyworth, PG., et al. 1991. J Clin Invest. 87: 352-6. PMID: 1985107
  3. 重组 47 千道尔顿细胞因子可恢复慢性肉芽肿病中的 NADPH 氧化酶。  |  Lomax, KJ., et al. 1989. Science. 245: 409-12. PMID: 2547247
  4. NADPH氧化酶p47phox siRNA可抑制apoE（-/-）小鼠外膜纤维母细胞的增殖和迁移。  |  Xu, F., et al. 2015. J Transl Med. 13: 38. PMID: 25628043
  5. p47phox 的 S-谷胱甘肽化能维持活化的中性粒细胞中超氧化物的生成。  |  Nagarkoti, S., et al. 2018. Biochim Biophys Acta Mol Cell Res. 1865: 444-454. PMID: 29195919
  6. 突触后p47phox调节海马的长期抑制。  |  Yi, JH., et al. 2018. Cell Discov. 4: 44. PMID: 30181899
  7. 通过基于片段的药物发现开发p47phox-p22phox蛋白-蛋白相互作用的抑制剂。  |  Solbak, SMØ., et al. 2020. J Med Chem. 63: 1156-1177. PMID: 31922756
  8. 化学合成一种已报道的p47phox/p22phox抑制剂并鉴定其不稳定性和不可重复性活性。  |  Zang, J., et al. 2022. Front Pharmacol. 13: 1075328. PMID: 36686674
  9. p67phox 基因的特征：用于慢性肉芽肿病产前诊断的基因组组织和限制性片段长度多态性分析。  |  Kenney, RT., et al. 1993. Blood. 82: 3739-44. PMID: 7903171
  10. 一个 SH3 结构域和富含脯氨酸的序列介导了吞噬细胞 NADPH 氧化酶复合物两个成分之间的相互作用。  |  Finan, P., et al. 1994. J Biol Chem. 269: 13752-5. PMID: 8188650
  11. p47-phox假基因携带着导致p47-phox缺陷型慢性肉芽肿病的最常见突变。  |  Görlach, A., et al. 1997. J Clin Invest. 100: 1907-18. PMID: 9329953
  12. 通过其内在荧光研究白细胞 NADPH 氧化酶亚基 p47(phox) 在活化过程中的构象变化。  |  Park, HS. and Park, JW. 1998. Biochim Biophys Acta. 1387: 406-14. PMID: 9748657

订购信息

产品名称产品编号规格价格数量收藏夹

p47phox 抗体 (D-10)

sc-17845
200 µg/ml
$316.00

p47phox (D-10): m-IgG Fc BP-HRP 套装

sc-528334
200 µg Ab; 10 µg BP
$354.00

p47phox (D-10): m-IgGκ BP-HRP 套装

sc-520683
200 µg Ab, 40 µg BP
$354.00

p47phox (D-10): m-IgG1 BP-HRP 套装

sc-542872
200 µg Ab; 20 µg BP
$354.00

p47phox 抗体 (D-10) AC

sc-17845 AC
500 µg/ml, 25% agarose
$416.00

p47phox 抗体 (D-10) HRP

sc-17845 HRP
200 µg/ml
$316.00

p47phox 抗体 (D-10) FITC

sc-17845 FITC
200 µg/ml
$330.00

p47phox 抗体 (D-10) PE

sc-17845 PE
200 µg/ml
$343.00

p47phox 抗体 (D-10) Alexa Fluor® 488

sc-17845 AF488
200 µg/ml
$357.00

p47phox 抗体 (D-10) Alexa Fluor® 546

sc-17845 AF546
200 µg/ml
$357.00

p47phox 抗体 (D-10) Alexa Fluor® 594

sc-17845 AF594
200 µg/ml
$357.00

p47phox 抗体 (D-10) Alexa Fluor® 647

sc-17845 AF647
200 µg/ml
$357.00

p47phox 抗体 (D-10) Alexa Fluor® 680

sc-17845 AF680
200 µg/ml
$357.00

p47phox 抗体 (D-10) Alexa Fluor® 790

sc-17845 AF790
200 µg/ml
$357.00

Can it detect the phosphorylation of p47phox in RAW264.7 after PMA stimulation by SDS-pages? How can I get a free 10ug samples? Thank you

Asked by: Mark1
Thank you for your question. This mouse monoclonal antibody, p47-phox (D-10): sc-17845, is not phospho-specific but it should be able to detect both the phosphorylated and non-phosphorylated forms of p47-phox in mouse samples. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-11-14

Can you recommend a positive control to use with p47-phox Antibody (D-10): sc-17845 for Western blot?

Asked by: jenni
We recommend Ramos: sc-2216, Daudi: sc-2415, HL-60: sc-2209, THP-1 : sc-2238 or RAW 264.7: sc-2211 whole cell lysates to use as positive controls as we have Western blot data showing specific detection of p47-phox in these cell lines.
Answered by: Technical Support 15
Date published: 2017-01-09
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_17845, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 101ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from buon funzionamentoBuon anticorpo in WB, dil 1:500. Forse un po' debole il segnale
Date published: 2025-05-30
Rated 4 out of 5 by from Good for WBWork nice in western blot analysis for rat renal tubular cells
Date published: 2023-09-13
Rated 5 out of 5 by from specific bandLoading 40 ug mouse spleen tissue lysates each lane.The specific band is at 47kda.Recommend use dilution 1:500 to 1:800.
Date published: 2017-08-26
Rated 5 out of 5 by from IB signal is strong and back ground is clear.I have tried this antibody with neutrophil lysate. The band is clear and no back groud. Very recommend.
Date published: 2017-08-15
Rated 4 out of 5 by from Quite good antibodyWe tested p47phox by western blotting analysis. 60 micrograms of mouse brain membrane lysates were resolved on 10% SDS-PAGE, and incubated over night with monoclonal p47phox antibody (1:1000). Secondary antibody utilized was anti-mouse sc-2005 antibody (1:5000). p47phox signal was detected by chemidoc (Biorad) and the signal was reached only after long exposition. Based on our experience p47phox is a quite good antibody.
Date published: 2017-07-20
Rated 5 out of 5 by from antibody resultsThis mono clone gives specific and clean WB, Great antibody!
Date published: 2017-06-01
Rated 5 out of 5 by from good qualityIt was used to detect p47-phox in rat brain. Bands were clear.
Date published: 2017-04-19
Rated 5 out of 5 by from Great antibody!The antibody for the WB of rat protein,the band is clear and special!
Date published: 2017-03-15
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_17845, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT
p47phox 抗体 (D-10) is rated 4.6 out of 5 by 13.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_17845, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 101ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。