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p38 alpha MAPK14 Antibody (9F12) - Western Blotting - Image 349991
p38α MAPK14 (9F12): sc-81621. K-562 (A), HL-60 (B), A-431 (C), MCF7 (D), NIH/3T3 (E) 和 RAW 309 Cr. 1 (F) 全细胞裂解液中 p38α MAPK14 表达的 Western 印迹分析.
p38α MAPK14 (9F12): sc-81621. K-562 (A), HL-60 (B), A-431 (C), MCF7 (D), NIH/3T3 (E) 和 RAW 309 Cr. 1 (F) 全细胞裂解液中 p38α MAPK14 表达的 Western 印迹分析.

p38 alpha MAPK14 抗体 (9F12): sc-81621

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说明书
  • p38 alpha MAPK14 抗体 9F12 是小鼠单克隆 IgG1 κ，p38 alpha MAPK14抗体, 在96篇文献中引用，规格为200 µg/ml
  • 针对全长重组p38 alpha MAPK14，human起源
  • p38 alpha MAPK14 抗体 (9F12) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 p38 alpha MAPK14
  • 抗p38 alpha MAPK14抗体（9F12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p38 alpha MAPK14 (9F12): sc-81621的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成p38 alpha MAPK14 抗体 (9F12)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
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研究信息

関連項目

额外p38 MAPK抗体，包括p38 alpha MAPK14, p38 beta MAPK11和p38 gamma MAPK12

p38 alpha MAPK14抗体（9F12）是一种小鼠单克隆IgG1 kappa轻链抗体，专门设计用于识别小鼠、大鼠和人类中的p38 alpha MAPK14，也称为p38α、CSBP、MXI2或MAP激酶14。p38 alpha MAPK14单克隆抗体（9F12）是针对全长重组 人类p38αMAPK14全长重组蛋白，可用于多种应用，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）。p38αMAPK14是细胞分裂素活化蛋白激酶（MAPK）家族的重要成员，其独特之处在于其单一的蛋白激酶结构域，该结构域对于其在细胞信号传导通路中的作用至关重要。p38αMAPK14的结构，特别是其激酶结构域和对镁作为辅因子的依赖性，对于其ATP依赖性磷酸化活性至关重要，通过向苏氨酸和酪氨酸残基添加磷酸基团来修饰靶蛋白。这种结构对于参与细胞粘附、扩散、分化、凋亡等关键生物过程至关重要。了解p38αMAPK14的复杂结构，研究人员就能阐明其在各种信号转导通路、细胞因子产生和细胞增殖中的作用。抗p38αMAPK14抗体（9F12）有非结合形式 以及多种结合形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）以及各种Alexa Fluor®结合物，可满足各种实验需求。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p38 alpha MAPK14 抗体 (9F12) 参考文献:

  1. 红细胞生成素的表达需要 p38α：应激激酶在红细胞生成中的作用。  |  Tamura, K., et al. 2000. Cell. 102: 221-31. PMID: 10943842
  2. Exip 是 p38 alpha 的一种新的替代剪接变体，可诱导 HeLa 细胞更早地发生凋亡。  |  Sudo, T., et al. 2002. Biochem Biophys Res Commun. 291: 838-43. PMID: 11866441
  3. p38丝裂原活化蛋白激酶成员应激活化蛋白激酶-3（SAPK3）的心脏表达和亚细胞定域。  |  Court, NW., et al. 2002. J Mol Cell Cardiol. 34: 413-26. PMID: 11991731
  4. 人类 p38alpha 丝裂原活化蛋白激酶的活性突变体。  |  Diskin, R., et al. 2004. J Biol Chem. 279: 47040-9. PMID: 15284239
  5. p38和一种p38相互作用蛋白对小鼠胃形成过程中E-cadherin的下调至关重要。  |  Zohn, IE., et al. 2006. Cell. 125: 957-69. PMID: 16751104
  6. 人 p38 MAP 激酶的分子克隆。  |  Han, J., et al. 1995. Biochim Biophys Acta. 1265: 224-7. PMID: 7696354
  7. 一种参与调节炎症细胞因子生物合成的蛋白激酶。  |  Lee, JC., et al. Nature. 372: 739-46. PMID: 7997261
  8. 一种新的丝裂原活化蛋白激酶（p38beta）的结构和功能特征。  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
  9. p38 gamma 的主要结构：p38 MAP 激酶组的新成员。  |  Li, Z., et al. 1996. Biochem Biophys Res Commun. 228: 334-40. PMID: 8920915

订购信息

产品名称产品编号规格价格数量收藏夹

p38 alpha MAPK14 抗体 (9F12)

sc-81621
200 µg/ml
$316.00

p38 alpha MAPK14 抗体 (9F12) AC

sc-81621 AC
500 µg/ml, 25% agarose
$416.00

p38 alpha MAPK14 抗体 (9F12) HRP

sc-81621 HRP
200 µg/ml
$316.00

p38 alpha MAPK14 抗体 (9F12) FITC

sc-81621 FITC
200 µg/ml
$330.00

p38 alpha MAPK14 抗体 (9F12) PE

sc-81621 PE
200 µg/ml
$343.00

p38 alpha MAPK14 抗体 (9F12) Alexa Fluor® 488

sc-81621 AF488
200 µg/ml
$357.00

p38 alpha MAPK14 抗体 (9F12) Alexa Fluor® 546

sc-81621 AF546
200 µg/ml
$357.00

p38 alpha MAPK14 抗体 (9F12) Alexa Fluor® 594

sc-81621 AF594
200 µg/ml
$357.00

p38 alpha MAPK14 抗体 (9F12) Alexa Fluor® 647

sc-81621 AF647
200 µg/ml
$357.00

p38 alpha MAPK14 抗体 (9F12) Alexa Fluor® 680

sc-81621 AF680
200 µg/ml
$357.00

p38 alpha MAPK14 抗体 (9F12) Alexa Fluor® 790

sc-81621 AF790
200 µg/ml
$357.00

Dear Sirs, Is m-IgG Fc BP-HRP (sc-525409) secondary antibody suitable for the p38 (sc-81621)? Thank you!

Asked by: Martomich
Thank you for your question. It would be helpful if you could call us directly at 800- 457-3801 option 2, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-04

What is the recommended positive control for p38alpha Antibody (9F12): sc-81621?

Asked by: Trav11
Thank you for your question. For p38alpha Antibody (9F12): sc-81621, we have several recommended positive controls, which include: MCF7 whole cell lysate (sc-2206), HL-60 whole cell lysate (sc-2209), or A-431 whole cell lysate (sc-2201). If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-02-01
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Rated 4 out of 5 by from Good antibodyMultiple bands were detected but still expected size band was detected.
Date published: 2021-12-03
Rated 5 out of 5 by from Excellent.This antibody works great for western blotting.Excellent.
Date published: 2019-07-30
Rated 2 out of 5 by from It does not work wellI had a even 1:500 dilution, and it did not work well as compared with other companies
Date published: 2018-04-03
Rated 4 out of 5 by from Good for IFThis p38α Antibody (9F12), sc-81621, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 4 out of 5 by from Very good in WB!!!Very good signal for my BT474 and HCT116 cells!!!! 15 ug lysate should be used at least...
Date published: 2018-02-01
Rated 4 out of 5 by from Use to detect p38 protein levelsThis antibody is very useful for protein detection in animal cell compartments. In particular, we used this antibody to detect protein levels in nuclear and cytosolic cell compartments in hippocampus and prefrontal cortex of mice and rats. In general, we are very satisfied with the obtained results, because Ab displayed high purity and clear bands in Western blot. Dilution up to 1:1000 for Western blot.
Date published: 2017-11-23
Rated 5 out of 5 by from Used to detect p38 protein levelsThis antibody is very useful for protein detection in animal cell compartments. In particular, we used this antibody to detect protein levels in nuclear and cytosolic cell compartments in hippocampus and prefrontal cortex of mice and rats. In general, we are very satisfied with the obtained results, because Ab displayed high purity and clear bands in Western blot. Dilution up to 1:1000 for Western blot.
Date published: 2017-11-23
Rated 3 out of 5 by from Good antibody for western blotThe batch of antibody I have worked well on HeLa, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. Observed: a weak specific band around 37kDa. The antibody was diluted 500 times into 5% BSA, into TBST with an overnight incubation at 4°C.
Date published: 2017-11-16
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p38 alpha MAPK14 抗体 (9F12) is rated 4.3 out of 5 by 14.
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