Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
P2Y1 CRISPR/Cas9 KO Plasmid (h): sc-401759
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P2Y1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the P2Y1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: P2Y1 Antibody (E-1): sc-377324
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P2Y1 CRISPR/Cas9 KO Plasmid (h) | sc-401759 | 20 µg | $397.00 |
Overview
P2RY1 encodes the human P2Y1 receptor, a G protein-coupled purinergic receptor activated primarily by extracellular ADP to initiate Gq/PLCβ signaling, IP3-mediated Ca2+ mobilization, and downstream PKC/MAPK pathway responses. P2Y1 contributes to regulation of platelet activation and aggregation, vascular tone, and intercellular communication in endothelial and immune-associated contexts through nucleotide sensing. In the nervous system, P2Y1 signaling can modulate glial activity and synaptic network dynamics, linking purinergic cues to inflammatory and neuromodulatory processes. Dysregulated P2RY1 activity has been associated with thrombotic and cardiovascular phenotypes and is studied in the broader context of neuroinflammation and tumor microenvironment signaling where extracellular nucleotides shape cell behavior.
P2Y1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the P2RY1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the P2RY1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the P2RY1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish P2Y1 protein expression.
This CRISPR knockout system enables efficient generation of P2RY1-deficient cell models for investigation of P2Y1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting P2RY1 exon(s) critical for P2Y1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple P2RY1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by P2Y1 CRISPR/Cas9 KO Plasmid (h) and P2Y1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the P2RY1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by P2Y1 HDR Plasmid (h) and P2Y1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by P2RY1 homology arms to support homology-directed repair at defined P2RY1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.