Date published: 2025-9-6

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CDKN1B/Kip1 p27 抗体 (SX53G8.5): sc-53871

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说明书
  • CDKN1B/Kip1 p27抗体(SX53G8.5)是小鼠单克隆IgG1 κ, 在16篇文献中引用,规格为200 µg/ml
  • 针对human来源的纯化GST-p27 Kip1融合蛋白
  • 推荐用于 mouse, rat 和 human 来源的p27 Kip1 WB, IP, IF 和 IHC(P)检测
  • 看 CDKN1B/Kip1 p27 (F-8): sc-1641,可查看有标记的 CDKN1B/Kip1 p27 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 CDKN1B/Kip1 p27 抗体 (SX53G8.5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CDKN1B/Kip1 p27 抗体 (SX53G8.5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

p27 Kip1 抗体(SX53G8. 5)是一种小鼠单克隆 IgG1 抗体,可通过免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学等多种应用检测小鼠、大鼠和人样本中的 p27 Kip1。p27 Kip1 作为细胞周期进展的负调控因子发挥着至关重要的作用,尤其是在 G1 期,p27 Kip1 通过与 D 型细胞周期蛋白和 Cdk4 结合抑制细胞周期蛋白依赖性激酶(Cdks)的活性,从而阻止细胞周期向 S 期过渡。这种调节对维持细胞正常生长和分裂至关重要,尤其是在对外部信号(如转化生长因子-β(TGFβ))做出反应时,TGFβ可诱导细胞进入G1停滞期。p27 Kip1 调节细胞周期进展的能力突显了它在抑制肿瘤方面的重要性,因为 p27 Kip1 的失调会导致细胞增殖失控和癌症发展。抗 p27 Kip1 抗体(SX53G8.5)是研究细胞周期动力学和各种疾病的分子机制的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

CDKN1B/Kip1 p27 抗体 (SX53G8.5)

sc-53871
200 µg/ml
$316.00

CDKN1B/Kip1 p27 (SX53G8.5): m-IgG Fc BP-HRP 套装

sc-537006
200 µg Ab; 10 µg BP
$354.00

CDKN1B/Kip1 p27 (SX53G8.5): m-IgGκ BP-HRP 套装

sc-534186
200 µg Ab; 40 µg BP
$354.00

CDKN1B/Kip1 p27 (SX53G8.5): m-IgG1 BP-HRP 套装

sc-544951
200 µg Ab; 20 µg BP
$354.00

I am using p27 (SX53G8.5) monoclonal antibody to stain snap-frozen sections. Would I be able to do a quick IF staining (to preserve the RNA) or would I need to use antigen retrieval procedures? Also, what is the recommended dilution?

Asked by: Amber12
Thank you for your question. If you are fixing in formalin or pfa that would cause cross-linking, I would suggest performing an antigen retrieval step. The recommended starting dilution is 1:50 for IF and IHC applications.
Answered by: Tech Service
Date published: 2020-02-13

I am using p27 (SX53G8.5): sc-53871 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works well for IHC-PGood signal in colorectal FFPE tissue. Stained cleanly at 1:400 following antigen retrieval in citrate buffer.
Date published: 2023-02-20
Rated 5 out of 5 by from Strong bands observed in western blot analysisStrong bands observed in western blot analysis of p27 expression in non-transfected and human p27 transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-04-27
Rated 4 out of 5 by from WB: positive results with mouse p27 transfectedWB: positive results with mouse p27 transfected 293T and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-04-17
Rated 4 out of 5 by from Robust band in WB using human p27 transfectedRobust band in WB using human p27 transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Worked well for WB for our p27 studies humanWorked well for WB for our p27 studies human thyroid cell lines derived from anaplastic carcinoma(CAL-62, 8305C), papillary carcinoma (B-CPAP, BHT-101,ONCO-DG1), follicular carcinoma (FTC-133, CGTH-W1),differentiated carcinoma (K1). -SCBT Publication Review
Date published: 2015-03-21
Rated 5 out of 5 by from WB: works well with Kip16 cellsWB: works well with Kip16 cells, PE25 cells, and HepG2 Liver cancer cells. -SCBT Publication Review
Date published: 2014-02-17
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CDKN1B/Kip1 p27 抗体 (SX53G8.5) is rated 4.7 out of 5 by 6.
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