Date published: 2025-9-6
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p-p38 MAPK Antibody (D-8) - Western Blotting - Image 44006
p-p38 MAPK (H-147): sc-7973. 未转染 293T: sc-117752 (A), 小鼠 p38α 转染 293T: sc-122319 (B) 和 Jurkat (C) 全细胞裂解液中 p38α MAPK14 表达的 Western 印迹分析.
p-p38 MAPK (H-147): sc-7973. 未转染 293T: sc-117752 (A), 小鼠 p38α 转染 293T: sc-122319 (B) 和 Jurkat (C) 全细胞裂解液中 p38α MAPK14 表达的 Western 印迹分析.

p-p38 MAPK 抗体 (D-8): sc-7973

4.5(19)
寫評論提問

说明书
  • p-p38 MAPK抗体(D-8)是小鼠单克隆IgM κ, 在571篇文献中引用，规格为200 µg/ml
  • 推荐用于 mouse, rat， human 和 来源的Tyr 182 phosphorylated p38 alpha MAPK14, p38 beta MAPK11 and p38 gamma MAPK12 WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 还可偶联琼脂糖 用于IP; 偶联FITC 用于IF, IHC(P) 和 FCM
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 看 p-p38 MAPK (E-1): sc-166182，可查看有标记的 p-p38 MAPK 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取p-p38 MAPK (D-8): sc-7973的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成p-p38 MAPK 抗体 (D-8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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基因信息
说明书与实验方案
研究信息

関連項目

此外 p38 MAPK 抗体包括 p38 alpha MAPK14, p38 beta MAPK11 和 p38 gamma MAPK12

p-p38 MAPK抗体(D-8)是一种IgM κ小鼠单克隆p-p38 MAPK抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源Tyr 182磷酸化的p38 α MAPK14、p38 β MAPK11和p38 γ MAPK12。p-p38 MAPK抗体(D-8)既可作为偶联抗p-p38 MAPK抗体形式，也可作为非偶联抗p-p38 MAPK抗体形式。MAP（有丝分裂原激活蛋白）激酶在许多生物学过程中发挥着重要作用，包括细胞粘附和扩散、细胞分化和凋亡。p38 α MAPK14、p38 β MAPK11和p38 γ MAPK12各含有一个蛋白激酶结构域，属于MAP激酶家族。它们在体内不同区域表达，在心脏中具有共同的表达模式，p38蛋白使用镁作为辅助因子催化靶蛋白的ATP依赖性磷酸化。通过它们的催化活性，p38 α MAPK14、p38 β MAPK11和p38 γ MAPK12参与细胞内的各种事件，包括信号转导途径、细胞因子产生以及细胞增殖和分化。p38蛋白在Thr和Tyr残基上发生磷酸化，这一事件被认为可以激活磷酸化蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-p38 MAPK 抗体 (D-8) 参考文献:

  1. 红细胞生成素的表达需要 p38α：应激激酶在红细胞生成中的作用。  |  Tamura, K., et al. 2000. Cell. 102: 221-31. PMID: 10943842
  2. Exip 是 p38 alpha 的一种新的替代剪接变体，可诱导 HeLa 细胞更早地发生凋亡。  |  Sudo, T., et al. 2002. Biochem Biophys Res Commun. 291: 838-43. PMID: 11866441
  3. p38丝裂原活化蛋白激酶成员应激活化蛋白激酶-3（SAPK3）的心脏表达和亚细胞定域。  |  Court, NW., et al. 2002. J Mol Cell Cardiol. 34: 413-26. PMID: 11991731
  4. 人类 p38alpha 丝裂原活化蛋白激酶的活性突变体。  |  Diskin, R., et al. 2004. J Biol Chem. 279: 47040-9. PMID: 15284239
  5. p38和一种p38相互作用蛋白对小鼠胃形成过程中E-cadherin的下调至关重要。  |  Zohn, IE., et al. 2006. Cell. 125: 957-69. PMID: 16751104
  6. 人 p38 MAP 激酶的分子克隆。  |  Han, J., et al. 1995. Biochim Biophys Acta. 1265: 224-7. PMID: 7696354
  7. 一种参与调节炎症细胞因子生物合成的蛋白激酶。  |  Lee, JC., et al. Nature. 372: 739-46. PMID: 7997261
  8. 一种新的丝裂原活化蛋白激酶（p38beta）的结构和功能特征。  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
  9. p38 gamma 的主要结构：p38 MAP 激酶组的新成员。  |  Li, Z., et al. 1996. Biochem Biophys Res Commun. 228: 334-40. PMID: 8920915

订购信息

产品名称产品编号规格价格数量收藏夹

p-p38 MAPK 抗体 (D-8)

sc-7973
200 µg/ml
$316.00

p-p38 MAPK 抗体 (D-8) AC

sc-7973 AC
500 µg/ml, 25% agarose
$416.00

p-p38 MAPK 抗体 (D-8) FITC

sc-7973 FITC
200 µg/ml
$330.00

p-p38 MAPK (D-8) 中和勝肽

sc-7973 P
100 µg/0.5 ml
$68.00

p-p38 MAPK 抗体 (D-8) TRITC

sc-7973 TRITC
200 µg/ml
$320.00

Should I use the secondary mouse antibody which detects IgM ?

Asked by: Susan_SJ
Thank you for your question. Yes, this p-p38 antibody is a mouse IgM and we would recommend using an anti-mouse IgM secondary antibody for detection. Alternatively, you could use our mouse IgG kappa binding protein (m-IgGκ BP), for example, sc-516102. This secondary binds specifically to the kappa light chain of mouse IgG and mouse IgM. A link to the product information on our website is below as a reference. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp
Answered by: Technical Support
Date published: 2017-06-26

I have detected p-p38 with this Ab (#7973), but there are two bands at 50 kDa & 25 kDa, not 38~40 kDa. Which band is p-p38? (you can see my band: https://www.researchgate.net/post/Is_there_any_one_who_detected_p-p38_protein_in_western_blot)

Asked by: Susan_SJ
Thank you for your question. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com, and we would be happy to look into this further with you. We want to make sure you're successful in your experiment!
Answered by: Tech Service
Date published: 2025-05-22

Can I use this mouse monoclonal antibody for IF or IHC staining of mouse tissue?

Asked by: Anna99
Yes. Staining on mouse tissue can be done by using a directly conjugated antibody. p-p38 Antibody (D-8): sc-7973 is available conjugated to fluoroscein (sc-7973 FITC), 200 µg/ml, and TRITC (sc-7973 TRITC, 200 µg/ml) for IF, IHC(P) and FCM.
Answered by: Tech Service 5
Date published: 2017-01-05
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Rated 5 out of 5 by from Great features for western blotThe antibody can give a nice clean band. It is a good antibody.
Date published: 2024-07-01
Rated 5 out of 5 by from western blotThe antibody can gave a nice clean band. It is a good antibody.
Date published: 2023-03-10
Rated 5 out of 5 by from western blotThe antibody can gave a nice clean band. It is a good antibody.
Date published: 2023-03-07
Rated 4 out of 5 by from Very good specificityThe antibody was able to recognize a specific band in FRTL5 rat thyroid cells. Used 1:400 in TBS-BSA 5%, both on at 4°C and 1h RT.
Date published: 2021-02-23
Rated 5 out of 5 by from GREAT for IHC !!We had excellent results!!! using pituitary slices
Date published: 2021-02-11
Rated 1 out of 5 by from Not workingI can't detect any band at 1:500 dilution overnight in 5%skim milk. Whole cell lysate, CMT93 cell line. Any advice ?
Date published: 2018-12-11
Rated 5 out of 5 by from Worked greatThe antibody can detect a specific band, at 1:1000. Happy with the antibody.
Date published: 2017-05-20
Rated 5 out of 5 by from clear for WBIt was used to detect p-p38 , Bands were clear for WB .
Date published: 2017-03-18
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p-p38 MAPK 抗体 (D-8) is rated 4.5 out of 5 by 19.
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