Date published: 2026-10-17
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p-eNOS Antibody (pT495.33) - Western Blotting - Image 138268
p-NOS3 抗体 (pT495. 33): sc-136519. 体外磷酸化前 (B) 和体外磷酸化后 (C) 人内皮细胞裂解物 (A) 和含有 Thr-495 的重组 NOS3 蛋白的 Western 印迹分析.
p-eNOS Antibody (pT495.33) - Western Blotting - Image 138268
p-NOS3 抗体 (pT495. 33): sc-136519. 体外磷酸化前 (B) 和体外磷酸化后 (C) 人内皮细胞裂解物 (A) 和含有 Thr-495 的重组 NOS3 蛋白的 Western 印迹分析.
p-eNOS Antibody (pT495.33) - Western Blotting - Image 375366
p-NOS3 抗体 (pT495. 33): sc-136519. CCRF-CEM (A), Jurkat (B), BJAB (C) 和 TK-1 (D) 全细胞裂解液中 p-NOS3 表达的 Western 印迹分析.
p-eNOS Antibody (pT495.33) - Western Blotting - Image 376236
p-NOS3 抗体 (pT495. 33): sc-136519. JAR (A), HEK293 (B) 和 NIH/3T3 (C) 全细胞裂解液中 NOS3 磷酸化的 Western 印迹分析.
p-NOS3 抗体 (pT495. 33): sc-136519. 体外磷酸化前 (B) 和体外磷酸化后 (C) 人内皮细胞裂解物 (A) 和含有 Thr-495 的重组 NOS3 蛋白的 Western 印迹分析.

p-eNOS 抗体 (pT495.33): sc-136519

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寫評論提問
说明书
  • p-eNOS抗体(pT495.33)是小鼠单克隆IgG2a κ, 在7篇文献中引用，规格为200 µg/ml
  • 针对含磷酸化Thr 495（NOS3 of human起源）的短氨基酸序列
  • 推荐用于检测mouse, rat和human来源的Thr 495 phosphorylated NOS3 of human origin and correspondingly Thr 492 phosphorylated NOS3 of mouse and rat origin，由WBIP提供; 也和以下物种反应，包括： canine
  • 还可偶联琼脂糖 用于IP; 偶联HRP 用于WB, IHC(P) 和ELISA
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-136519 X, 200 µg/0.1 ml)
  • 目前，我们还没有完成p-eNOS 抗体 (pT495.33)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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関連項目

附加NOS抗体，包括NOS1, NOS2, NOS3和pan NOS

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-eNOS 抗体 (pT495.33) 参考文献:

  1. NOS3磷酸化减少导致去氧皮质酮醋酸盐盐高血压大鼠肠系膜动脉NO/cGMP信号传导减弱。  |  Sasser, JM., et al. 2004. Hypertension. 43: 1080-5. PMID: 14993198
  2. 乳腺癌细胞系和Src转化细胞中NOS3的酪氨酸磷酸化。  |  Takenouchi, Y., et al. 2004. Oncol Rep. 11: 1059-62. PMID: 15069547
  3. 人类内皮细胞NOS3 Glu298Asp变异产生的生化后果：囊泡定位改变，剪切力反应受损。  |  Joshi, MS., et al. 2007. FASEB J. 21: 2655-63. PMID: 17449720
  4. Akt激活NOS3并分别恢复H2O2胁迫人心脏微血管内皮屏障的完整性。  |  Dossumbekova, A., et al. 2008. Am J Physiol Heart Circ Physiol. 295: H2417-26. PMID: 18931031
  5. Akt1介导厚升支中嘌呤受体依赖性NOS3的激活。  |  Silva, GB. and Garvin, JL. 2009. Am J Physiol Renal Physiol. 297: F646-52. PMID: 19570880
  6. 血管紧张素II通过AT（2）受体和Akt1依赖性一氧化氮合成酶3（NOS3）激活刺激厚升支NO的产生。  |  Herrera, M. and Garvin, JL. 2010. J Biol Chem. 285: 14932-14940. PMID: 20299462
  7. 人类内皮基因多态性对高血糖细胞反应的影响：NOS3（Glu298Asp）和ACE（I/D）多态性的作用。  |  Joshi, MS., et al. 2011. Diab Vasc Dis Res. 8: 276-83. PMID: 21844127
  8. 对抗 Fas 的敏感性与 NOS-3 过表达的 HepG2 细胞中增加的 cathepsin D 活性和肾上腺素还原酶表达无关。  |  Linares, CI., et al. 2015. Biochim Biophys Acta. 1853: 1182-94. PMID: 25712867
  9. 血浆凝血素通过增强巨噬细胞NOS3的功能，提高肺部对肺炎的防御能力。  |  Yang, Z., et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L11-6. PMID: 25957291
  10. 大麻二酚通过增强人结肠癌细胞中NOS3和SOD2诱导的自噬克服奥沙利铂耐药性。  |  Jeong, S., et al. 2019. Cancers (Basel). 11: PMID: 31195721

订购信息

产品名称产品编号规格价格数量收藏夹

p-eNOS 抗体 (pT495.33)

sc-136519
200 µg/ml
$322.00

p-eNOS 抗体 (pT495.33) X

sc-136519 X
200 µg/0.1 ml
$322.00

p-eNOS 抗体 (pT495.33) AC

sc-136519 AC
500 µg/ml, 25% agarose
$424.00

p-eNOS 抗体 (pT495.33) HRP

sc-136519 HRP
200 µg/ml
$322.00

what kinda stripping buffer are u using? My buffer is configured 14.4M 2-mercaptoethanol, 10% SDS, 1M Tris-HCl[pH6.7], MilliQ.

Asked by: ryo k
Thank you for your question. We sell our own proprietary Western Blot Stripping Buffer, 500 ml sc-281698. The buffer components are not available.
Answered by: Technical Support
Date published: 2019-02-06

Are there any issues with using sc-136519: p-NOS3 (pT495.33) mouse monoclonal primary antibody on mouse samples?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-136519 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by Tanusree from Good antibodyWorked well with 1:500 dilution with mouse brain tissue.
Date published: 2018-01-02
Rated 4 out of 5 by Tsen from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 4 out of 5 by 小新贝贝 from Good antibody!1:250 diluted for wb of rat protein ,the band is strong and the background is clear.
Date published: 2017-07-29
Rated 4 out of 5 by morgile from Good resultsWe got a good result of NOS3 protein containing Thr-495 by Western blot analysis,and the band is clear.
Date published: 2017-02-23
Rated 4 out of 5 by FL from Good results by Western blot analysis ofGood results by Western blot analysis of human endothelial cell lysate and recombinant NOS3 protein containing Thr-495 before in vitro phosphorylation and after in vitro phosphorylation. -SCBT QC
Date published: 2014-01-15
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p-eNOS 抗体 (pT495.33) is rated 4.0 out of 5 by 5.
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