p-MEK-1 Antibody (47.Ser 222): sc-136542

p-MEK-1 Antibody (47.Ser 222) is a mouse monoclonal IgA antibody that detects p-MEK-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). p-MEK-1 (47.Ser 222) antibody is available in a non-conjugated form and specifically recognizes Ser 222 phosphorylated MEK-1 and MEK-2, which are critical components of the MAP kinase signaling pathway. MEK-1, also known as MAP2K1, plays a vital role in cellular signaling by acting as a dual-specificity kinase that phosphorylates both threonine and tyrosine residues on downstream targets, particularly extracellular signal-regulated kinases (ERKs). This phosphorylation activates ERKs, which drive various cellular processes, including proliferation, differentiation, and survival. Precise regulation of MEK-1 activity through phosphorylation is crucial, as dysregulation can lead to oncogenic transformations and contribute to various diseases, including cancer. Understanding p-MEK-1 function and regulation is important for developing targeted therapies and advancing knowledge of signal transduction mechanisms.

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p-MEK-1 Antibody (47.Ser 222) References:

1. MEK-1/2 inhibition prevents 5-lipoxygenase translocation in N-formylpeptide-challenged human neutrophils.  |  Boden, SE., et al. 2000. Int J Biochem Cell Biol. 32: 1069-74. PMID: 11091139
2. Importance of MEK-1/-2 signaling in monocytic and granulocytic differentiation of myeloid cell lines.  |  Miranda, MB., et al. 2002. Leukemia. 16: 683-92. PMID: 11960350
3. Baicalein inhibits Raf-1-mediated phosphorylation of MEK-1 in C6 rat glioma cells.  |  Nakahata, N., et al. 2003. Eur J Pharmacol. 461: 1-7. PMID: 12568909
4. MEK-1 activates C-Raf through a Ras-independent mechanism.  |  Leicht, DT., et al. 2013. Biochim Biophys Acta. 1833: 976-86. PMID: 23360980
5. Orexin-A protects SH-SY5Y cells against H2O2-induced oxidative damage via the PI3K/MEK1/2/ERK1/2 signaling pathway.  |  Wang, CM., et al. 2018. Int J Immunopathol Pharmacol. 32: 2058738418785739. PMID: 29983082
6. B-Raf-dependent regulation of the MEK-1/mitogen-activated protein kinase pathway in PC12 cells and regulation by cyclic AMP.  |  Vaillancourt, RR., et al. 1994. Mol Cell Biol. 14: 6522-30. PMID: 7935374
7. RAS and RAF-1 form a signalling complex with MEK-1 but not MEK-2.  |  Jelinek, T., et al. 1994. Mol Cell Biol. 14: 8212-8. PMID: 7969158
8. MEK-1 phosphorylation by MEK kinase, Raf, and mitogen-activated protein kinase: analysis of phosphopeptides and regulation of activity.  |  Gardner, AM., et al. 1994. Mol Biol Cell. 5: 193-201. PMID: 8019005
9. Identification of 2 serine residues of MEK-1 that are differentially phosphorylated during activation by raf and MEK kinase.  |  Yan, M. and Templeton, DJ. 1994. J Biol Chem. 269: 19067-73. PMID: 8034665
10. The hCSE1/CAS protein is phosphorylated by HeLa extracts and MEK-1: MEK-1 phosphorylation may modulate the intracellular localization of CAS.  |  Scherf, U., et al. 1998. Biochem Biophys Res Commun. 250: 623-8. PMID: 9784396

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-MEK-1 Antibody (47.Ser 222) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-p-MEK-1 Antibody (47.Ser 222) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-MEK-1 Antibody (47.Ser 222) Specifications

Alternate Product Name: MAP2K1

Product Clone Name: 47.Ser 222

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgA

Amino Acids: Ser 222

Epitope Target: MEK-1

Epitope Species: human

Product Reactivity/Detection: Ser 222 phosphorylated MEK-1 and Ser 222 phosphorylated MEK-2 of mouse, rat and human origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgA in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for p-MEK-1 Antibody (47.Ser 222): N/A

The preferred MEK-1 antibody is MEK-1 Antibody (H-8): sc-6250.