Date published: 2025-9-6
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p-Cdk5 Antibody (C-7) - Western Blotting - Image 294428
未经处理 (A, C) 和经λ蛋白磷酸酶 (sc-200312A) 处理 (B, D) 的 SK-N-MC 全细胞裂解液中 Cdk5 磷酸化的 Western 印迹分析. 测试的抗体包括 p-Cdk5 抗体 (C-7): sc-377558 (A, B) 和 Cdk5 (J-3): sc-6247 (C, D).
p-Cdk5 Antibody (C-7) - Western Blotting - Image 294428
未经处理 (A, C) 和经λ蛋白磷酸酶 (sc-200312A) 处理 (B, D) 的 SK-N-MC 全细胞裂解液中 Cdk5 磷酸化的 Western 印迹分析. 测试的抗体包括 p-Cdk5 抗体 (C-7): sc-377558 (A, B) 和 Cdk5 (J-3): sc-6247 (C, D).
未经处理 (A, C) 和经λ蛋白磷酸酶 (sc-200312A) 处理 (B, D) 的 SK-N-MC 全细胞裂解液中 Cdk5 磷酸化的 Western 印迹分析. 测试的抗体包括 p-Cdk5 抗体 (C-7): sc-377558 (A, B) 和 Cdk5 (J-3): sc-6247 (C, D).

p-Cdk5 抗体 (C-7): sc-377558

4.4(7)
寫評論提問

说明书
  • p-Cdk5抗体(C-7)是小鼠单克隆IgG3 κ, 在9篇文献中引用，规格为200 µg/ml
  • 与human的Ser 159氨基酸残基的Cdk5
  • 推荐用于 mouse, rat 和 human 来源的Ser 159 phosphorylated Cdk5 WB, IP, IF 和 ELISA检测; 也和以下物种反应，包括： 和 equine, bovine and porcine
  • 关于如何获取p-Cdk5 (C-7): sc-377558的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP是p-Cdk5 Antibody (C-7) 适用于 WB 应用。 的首选辅助检测试剂，该试剂现与p-Cdk5 Antibody (C-7) 搭配使用（请参阅下面的订购信息）。

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研究信息

関連項目

附加Cdk抗体，包括Cdk1, Cdk11, Cdk2, Cdk3, Cdk4, Cdk5, Cdk6, Cdk7, Cdk8和Cdk9

p-Cdk5抗体（C-7）是一种IgG3 κ小鼠单克隆p-Cdk5抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Ser 159磷酸化Cdk5。p-Cdk5抗体（C-7）以非偶联的抗p-Cdk5抗体形式存在。周期蛋白依赖性激酶5（Cdk5）仅以活性形式存在于神经细胞中。与其他Cdk家族成员一样，Cdk5的催化活性受到p35结合和磷酸化的影响。丝氨酸159残基是Cdk5激活激酶的主要磷酸化靶标。Cdk5需要p35结合和丝氨酸159的磷酸化才能达到最大激活速率。此外，酪蛋白激酶I（而非酪蛋白激酶II）可以在体外磷酸化并激活Cdk5。c-Abl对Cdk5的磷酸化发生在酪氨酸15上，并增强p35/Cdk5激酶活性。活性c-Abl激酶导致Cdk5酪氨酸磷酸化，并且这种磷酸化作用被Cdk5和Abl酶底物（CABLES）增强。丝氨酸159或酪氨酸15的磷酸化都会显著增加Cdk5的激活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Cdk5 抗体 (C-7) 参考文献:

  1. 通过磷酸化调节细胞周期蛋白依赖性激酶 5 的催化活性。  |  Sharma, P., et al. 1999. Proc Natl Acad Sci U S A. 96: 11156-60. PMID: 10500146
  2. 缆索连接 Cdk5 和 c-Abl，促进 Cdk5 酪氨酸磷酸化, 激酶上调和神经元生长。  |  Zukerberg, LR., et al. 2000. Neuron. 26: 633-46. PMID: 10896159
  3. 神经元 cdc2 样激酶：一种主要在神经元中表达的 cdc2 相关蛋白激酶。  |  Hellmich, MR., et al. 1992. Proc Natl Acad Sci U S A. 89: 10867-71. PMID: 1279696
  4. CDK5：细胞凋亡和细胞存活的关键调节因子。  |  Roufayel, R. and Murshid, N. 2019. Biomedicines. 7: PMID: 31698798
  5. CDK5 通过磷酸化积极调节胰腺癌细胞中的 Notch1 信号转导。  |  Chu, Q., et al. 2021. Cancer Med. 10: 3689-3699. PMID: 33960694
  6. Cdk5在神经系统疾病中的作用。  |  Ao, C., et al. 2022. Front Cell Neurosci. 16: 951202. PMID: 35966199
  7. CDK5-PRMT1-WDR24 信号级联促进了 mTORC1 信号转导和肿瘤生长。  |  Yin, S., et al. 2023. Cell Rep. 42: 112316. PMID: 36995937
  8. CDK5对NLGN3和突触Rho-GEF信号通路的调节。  |  Jeong, J., et al. 2023. J Neurosci. 43: 7264-7275. PMID: 37699715
  9. 一种脑特异性细胞周期蛋白依赖性激酶 5 激活剂。  |  Lew, J., et al. 1994. Nature. 371: 423-6. PMID: 8090222
  10. 大鼠脊髓中的 cdc2 样激酶特异性磷酸化神经丝蛋白中的 KSPXK 矩阵：分离与表征。  |  Shetty, KT., et al. 1993. Proc Natl Acad Sci U S A. 90: 6844-8. PMID: 8341707

订购信息

产品名称产品编号规格价格数量收藏夹

p-Cdk5 抗体 (C-7)

sc-377558
200 µg/ml
$316.00

p-Cdk5 (C-7): m-IgG3 BP-HRP 套装

sc-550591
200 µg Ab; 40 µg BP
$354.00

p-Cdk5 (C-7) 中和勝肽

sc-377558 P
100 µg/0.5 ml
$68.00

Specific bands were detected near 50kDa and 25kDa. What do these bands represent?

Asked by: band
Thank you for your question. You can contact our distributor in Korea. https://www.scbt.com/resources/distributors In one case, the primary antibody heavy chain and light chain have been detected. Or you can conduct WB by only adding the seconday antibody, and the correct result shoud be negative.
Answered by: Ling Chen
Date published: 2022-06-09

What application is the blocking peptide sc-377558 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Great with western blottingThis is a very clean antibody on western blotting. Recognized one band in HL60 and SKNBE2 cells.
Date published: 2018-11-08
Rated 4 out of 5 by from GoodThis antibody works well in western blotting by using cultured cells.
Date published: 2018-01-09
Rated 5 out of 5 by from Good and cleanUsed at an dilution of 1:250. Left lane loaded with protein standard marker 250, 130, 100, 55, 35, 25 kDa bands, Right lane loaded 30ug LNCaP lysate
Date published: 2017-10-27
Rated 4 out of 5 by from Worked but wasn't the cleaniestI was given a free trial for this antibody and it worked as directed, however the bands that showed were not the cleaniest. May be an animal issue and not an antibody issue
Date published: 2017-07-24
Rated 4 out of 5 by from goodA specific CDK5 band could be easily seen in mouse brain and cell lines lystaes.
Date published: 2017-05-30
Rated 5 out of 5 by from Great monoclonal for p-Cdk-5Nice strong Western blot data using this antibody, it's a solid clone that gives reliable results.
Date published: 2017-01-26
Rated 4 out of 5 by from Good specificity in untreated and lambdaGood specificity in untreated and lambda protein phosphatase treated SK-N-MC whole cell lysates.
Date published: 2013-02-26
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p-Cdk5 抗体 (C-7) is rated 4.4 out of 5 by 7.
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