OTUD5 CRISPR/Cas9 KO Plasmid (m): sc-424913
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OTUD5 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OTUD5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OTUD5 CRISPR/Cas9 KO Plasmid (m) | sc-424913 | 20 µg | $397.00 |
Overview
Otud5 encodes OTUD5, an ovarian tumor (OTU) family deubiquitinase that removes ubiquitin chains from protein substrates to modulate their stability and signaling output. OTUD5 activity intersects with ubiquitin-dependent control of DNA damage responses, innate immune signaling, and stress-regulated transcriptional programs, shaping how cells coordinate checkpoint activation and inflammatory cues. In mouse models and cellular systems, perturbation of OTUD5-associated deubiquitination can alter proteostasis and pathway flux in processes linked to neurodevelopmental and immune-relevant phenotypes. These features make Otud5 a useful node for dissecting ubiquitin editing mechanisms that tune signal transduction and genome integrity.
OTUD5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Otud5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Otud5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Otud5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OTUD5 protein expression.
This CRISPR knockout system enables efficient generation of Otud5-deficient cell models for investigation of OTUD5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Otud5 exon(s) critical for OTUD5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Otud5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OTUD5 CRISPR/Cas9 KO Plasmid (m) and OTUD5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Otud5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OTUD5 HDR Plasmid (m) and OTUD5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Otud5 homology arms to support homology-directed repair at defined Otud5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.