Date published: 2025-9-7
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OSX Antibody (F-3) - Western Blotting - Image 378442
OSX 抗体 (F-3): sc-393325. TF-1 (A), TK-1 (B) 和 HOS (C) 全细胞裂解液中 OSX 表达的 Western 印迹分析.
OSX Antibody (F-3) - Western Blotting - Image 378442
OSX 抗体 (F-3): sc-393325. TF-1 (A), TK-1 (B) 和 HOS (C) 全细胞裂解液中 OSX 表达的 Western 印迹分析.
OSX Antibody (F-3) - Western Blotting - Image 320287
OSX 抗体 (F-3): sc-393325. NCI-H929 (A), TF-1 (B) 和 U-2 OS (C) 全细胞裂解液中 OSX 表达的 Western 印迹分析.
OSX Antibody (F-3) - Western Blotting - Image 377658
OSX 抗体 (F-3): sc-393325. TF-1 全细胞裂解液中 OSX 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
OSX 抗体 (F-3): sc-393325. TF-1 (A), TK-1 (B) 和 HOS (C) 全细胞裂解液中 OSX 表达的 Western 印迹分析.

OSX 抗体 (F-3): sc-393325

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说明书
  • OSX 抗体 F-3 是小鼠单克隆 IgG1 κ，OSX抗体, 在66篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列172-268位于human物种的OSX的内部区域内
  • OSX 抗体 (F-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 OSX
  • 抗OSX抗体（F-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-393325 X, 200 µg/0.1 ml)
  • 关于如何获取OSX (F-3): sc-393325的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是OSX Antibody (F-3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与OSX Antibody (F-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

OSX抗体（F-3）是一种IgG1 κ小鼠单克隆OSX抗体（也被称为Sp7抗体或Osterix抗体），可通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类来源的OSX蛋白。OSX抗体（F-3）既有非偶联抗OSX抗体形式，也有多种偶联形式的抗OSX抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Osterix（OSX）是一种含有锌指的转录激活因子，它在所有发育中的骨骼中都有独特的表达，对成骨细胞的分化至关重要。特别的是，OSX参与了成骨细胞的分化过程，成骨细胞是专门负责骨骼形成的细胞。OSX是一种核蛋白，它与GC盒启动子元件结合，并从含有功能识别位点的基因中激活mRNA的合成。在OSX基因敲除小鼠的膜性骨骼元素中，骨膜和间充质细胞无法分化为成骨细胞。随后，OSX基因敲除小鼠的间充质细胞无法沉积骨基质，也无法形成骨骼。Cox-2缺乏与OSX表达的减少相关，这表明Cox-2可能诱导OSX来介导骨骼修复。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

OSX 抗体 (F-3) 参考文献:

  1. 新型含锌转录因子 osterix 是成骨细胞分化和骨形成所必需的。  |  Nakashima, K., et al. 2002. Cell. 108: 17-29. PMID: 11792318
  2. 骨形态发生蛋白-2 可增强软骨细胞中的 osterix 基因表达。  |  Yagi, K., et al. 2003. J Cell Biochem. 88: 1077-83. PMID: 12647290
  3. BMP-2 诱导的 Osterix 表达由 Dlx5 介导，但与 Runx2 无关。  |  Lee, MH., et al. 2003. Biochem Biophys Res Commun. 309: 689-94. PMID: 12963046
  4. 骨巨细胞瘤基质瘤细胞中前成骨细胞标志物及 Cbfa-1 和 Osterix 基因转录本的表达。  |  Huang, L., et al. 2004. Bone. 34: 393-401. PMID: 15003787
  5. 胚胎成骨过程中硬骨蛋白和sterix表达的时空缔合作用和骨形态发生蛋白调控。  |  Ohyama, Y., et al. 2004. Endocrinology. 145: 4685-92. PMID: 15217980
  6. 地塞米松对原代大鼠成骨细胞分化过程中 Cbfa1, Osterix 和骨基质蛋白基因表达的诱导作用  |  Igarashi, M., et al. 2004. J Mol Histol. 35: 3-10. PMID: 15323344
  7. BMP-2和胰岛素样生长因子-I通过MAPK和蛋白激酶D信号通路介导人间质干细胞中Osterix（Osx）的表达。  |  Celil, AB. and Campbell, PG. 2005. J Biol Chem. 280: 31353-9. PMID: 16000303
  8. 肿瘤坏死因子对 osterix（Osx，Sp7）启动子的转录因子调控确定了丝裂原活化蛋白激酶和 NF kappa B 途径的不同作用。  |  Lu, X., et al. 2006. J Biol Chem. 281: 6297-306. PMID: 16410254
  9. p38 丝裂原活化蛋白激酶通过 osterix 调节成骨细胞分化。  |  Wang, X., et al. 2007. Endocrinology. 148: 1629-37. PMID: 17185377
  10. 转录因子 Osterix 与 RNA 螺旋酶 A 直接相互作用。  |  Amorim, BR., et al. 2007. Biochem Biophys Res Commun. 355: 347-51. PMID: 17303075
  11. 奥斯特里克斯（Osx，Sp7）启动子对奥斯特里克斯的调控及其对甲状旁腺激素的抑制。  |  Barbuto, R. and Mitchell, J. 2013. J Mol Endocrinol. 51: 99-108. PMID: 23682129
  12. 颌骨母细胞中 BMP-2 诱导的 Dspp 转录是由 Dlx3/Osx 信号通路介导的。  |  Yang, G., et al. 2017. Sci Rep. 7: 10775. PMID: 28883412

订购信息

产品名称产品编号规格价格数量收藏夹

OSX 抗体 (F-3)

sc-393325
200 µg/ml
$316.00

OSX (F-3): m-IgGκ BP-HRP 套装

sc-523905
200 µg Ab, 40 µg BP
$354.00

OSX (F-3): m-IgG1 BP-HRP 套装

sc-533622
200 µg Ab; 20 µg BP
$354.00

OSX 抗体 (F-3) X

sc-393325 X
200 µg/0.1 ml
$316.00

OSX 抗体 (F-3) AC

sc-393325 AC
500 µg/ml, 25% agarose
$416.00

OSX 抗体 (F-3) HRP

sc-393325 HRP
200 µg/ml
$316.00

OSX 抗体 (F-3) FITC

sc-393325 FITC
200 µg/ml
$330.00

OSX 抗体 (F-3) PE

sc-393325 PE
200 µg/ml
$343.00

OSX 抗体 (F-3) Alexa Fluor® 488

sc-393325 AF488
200 µg/ml
$357.00

OSX 抗体 (F-3) Alexa Fluor® 546

sc-393325 AF546
200 µg/ml
$357.00

OSX 抗体 (F-3) Alexa Fluor® 594

sc-393325 AF594
200 µg/ml
$357.00

OSX 抗体 (F-3) Alexa Fluor® 647

sc-393325 AF647
200 µg/ml
$357.00

OSX 抗体 (F-3) Alexa Fluor® 680

sc-393325 AF680
200 µg/ml
$357.00

OSX 抗体 (F-3) Alexa Fluor® 790

sc-393325 AF790
200 µg/ml
$357.00

Which second antibody are recommended for IHC in mouse bone tissues?

Asked by: Luhan
Thank you for your question. Here is a recommended secondary : https://www.scbt.com/p/m-igg1-bp-b
Answered by: BlakeJ
Date published: 2025-07-23

Which secondary antibodies do you recommend for IF staining in mouse tissues?

Asked by: jackie34
Thank you for your question. You can find our anti-mouse secondary binding proteins by following this link: https://www.scbt.com/browse/Secondary-Antibodies/_/N-1hoaxk4 OSX Antibody (F-3): sc-393325 is a kappa light chain antibody, so any of our kapppa binding proteins such as m-IgGκ BP-CFL 488 sc-516176 would be suitable.
Answered by: Tech Support Europe
Date published: 2019-11-22

Are there any issues with non-specific staining when using this mouse monoclonal antibody on mouse tissue samples in IF or IHC?

Asked by: AbPolly
Thank you for your question. This antibody is recommended for use with mouse samples in IF and IHC. To eliminate any potential issues with non-specific binding of the anti-mouse secondary, we recommend using the directly conjugated version of the primary. This antibody is available conjugated to PE, FITC, AF488 and AF647.
Answered by: Technical Support 15
Date published: 2024-07-29
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Rated 4 out of 5 by from Good antibodyThe band is clear but not so strong. You need a good ECL.
Date published: 2018-04-06
Rated 4 out of 5 by from One band with high backgroundI had a trial sample for this antibody, and got one band (~41kDa) in mouse periodontal stem cell lysate. Maybe because of the homology, there are several background bands.
Date published: 2017-08-21
Rated 4 out of 5 by from seems goodIt worked well for IHC in mouse femur section though the stability of this product still needs to be proved.
Date published: 2017-03-08
Rated 4 out of 5 by from good quanlityWe observed two bands of OSX in WB. Dilution of primary antibody is 1: 500
Date published: 2017-03-06
Rated 5 out of 5 by from Good AntibodyOSX Antibody Neurogenin 3 antibody could be performed under isothermal conditions, its specificity was good and the sensitivity. We were be quite content with the antibody.
Date published: 2017-02-13
Rated 5 out of 5 by from Between samples of rat bone marrow mesenchymal steWe observed the work main antibody dilution was 1:500. Between samples of rat bone marrow mesenchymal stem cells.
Date published: 2017-02-09
Rated 5 out of 5 by from Suitable for immunofluorescenceWe used sc-393325 to make immunofluorescence, and the staining pattern was very strong.
Date published: 2017-02-07
Rated 5 out of 5 by from Positive resultsObserved positive WB results with OSX Antibody (F-3)
Date published: 2017-01-18
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OSX 抗体 (F-3) is rated 4.5 out of 5 by 13.
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