Date published: 2025-9-5
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osteopontin/OPN/SPP1 Antibody (LFMb-14) - Western Blotting - Image 32809
OPN 抗体 (LFMb-14): sc-73631. HOS (A), U-87 MG (B) 和 Caki-1 (C) 全细胞裂解液中 OPN 表达的 Western 印迹分析.
OPN 抗体 (LFMb-14): sc-73631. HOS (A), U-87 MG (B) 和 Caki-1 (C) 全细胞裂解液中 OPN 表达的 Western 印迹分析.

osteopontin/OPN/SPP1 抗体 (LFMb-14): sc-73631

4.7(7)
寫評論提問

说明书
  • osteopontin/OPN/SPP1抗体(LFMb-14)是小鼠单克隆IgG2b κ, 在61篇文献中引用，规格为200 µg/ml
  • 针对全长重组OPN，human起源
  • 抗-osteopontin/OPN/SPP1 抗体 (LFMb-14) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 OPN
  • 抗osteopontin/OPN/SPP1抗体（LFMb-14）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取osteopontin/OPN/SPP1 (LFMb-14): sc-73631的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 osteopontin/OPN/SPP1 抗体 (LFMb-14) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与osteopontin/OPN/SPP1 抗体 (LFMb-14) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

OPN抗体（LFMb-14）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）等方法检测小鼠、大鼠和人类样本中的骨桥蛋白（OPN）。抗OPN抗体（LFMb-14）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。骨桥蛋白（OPN）又称骨唾液蛋白1、尿结石蛋白、SPP1、Eta-1、肾桥蛋白和尿桥蛋白，是一种重要的细胞外基质细胞粘附磷糖蛋白，在各种生物过程中发挥着重要作用。OPN的关键方面之一是翻译后修饰，这会导致分子量和功能特性的变化。这些修饰，如磷酸化作用和糖基化作用，对于调节OPN与其他蛋白质的相互作用以及细胞粘附、迁移和免疫反应调节等生物活性至关重要。翻译后修饰使OPN能够参与各种生理和病理过程，包括组织重塑、伤口愈合和肿瘤进展，使OPN成为健康和疾病中至关重要的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

osteopontin/OPN/SPP1 抗体 (LFMb-14)

sc-73631
200 µg/ml
$316.00

osteopontin/OPN/SPP1 (LFMb-14): m-IgG Fc BP-HRP 套装

sc-525701
200 µg Ab; 10 µg BP
$354.00

osteopontin/OPN/SPP1 (LFMb-14): m-IgG2b BP-HRP 套装

sc-549626
200 µg Ab; 10 µg BP
$354.00

osteopontin/OPN/SPP1 抗体 (LFMb-14) AC

sc-73631 AC
500 µg/ml, 25% agarose
$416.00

osteopontin/OPN/SPP1 抗体 (LFMb-14) HRP

sc-73631 HRP
200 µg/ml
$316.00

osteopontin/OPN/SPP1 抗体 (LFMb-14) FITC

sc-73631 FITC
200 µg/ml
$330.00

osteopontin/OPN/SPP1 抗体 (LFMb-14) PE

sc-73631 PE
200 µg/ml
$343.00

osteopontin/OPN/SPP1 抗体 (LFMb-14) Alexa Fluor® 488

sc-73631 AF488
200 µg/ml
$357.00

osteopontin/OPN/SPP1 抗体 (LFMb-14) Alexa Fluor® 546

sc-73631 AF546
200 µg/ml
$357.00

osteopontin/OPN/SPP1 抗体 (LFMb-14) Alexa Fluor® 594

sc-73631 AF594
200 µg/ml
$357.00

osteopontin/OPN/SPP1 抗体 (LFMb-14) Alexa Fluor® 647

sc-73631 AF647
200 µg/ml
$357.00

osteopontin/OPN/SPP1 抗体 (LFMb-14) Alexa Fluor® 680

sc-73631 AF680
200 µg/ml
$357.00

osteopontin/OPN/SPP1 抗体 (LFMb-14) Alexa Fluor® 790

sc-73631 AF790
200 µg/ml
$357.00

Is it recommended for WB and IHC in mouse liver?

Asked by: Bea31
Thank you for your question. Anti-osteopontin/OPN/SPP1 Antibody (LFMb-14) is recommended for detection of OPN of various cell and tissue types from mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM and will also work on liver samples.
Answered by: Tech Support Europe
Date published: 2022-06-13

Is OPN Antibody (LFMb-14): sc-73631 available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at specialorders@scbt.com Alternatively, consider using OPN (AKm2A1), which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/opn-antibody-akm2a1)
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 5 out of 5 by from Great productStrong western blot data; also nice IHC staining !!
Date published: 2017-01-10
Rated 5 out of 5 by from Especially good for IHCThis antibody is well-cited for a variety of applications but is especially good in IHC. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2016-12-22
Rated 5 out of 5 by from Used on a regular basis and published as wellUsed on a regular basis and published as well. They are working great for us.
Date published: 2015-05-28
Rated 4 out of 5 by from IHC: pfa fixed murine kidney samplesIHC: pfa fixed murine kidney samples. -SCBT Publication Review
Date published: 2015-04-03
Rated 4 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the OPN protein via Western Blot analysis. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Clean bands in western blot analysis of OPNClean bands in western blot analysis of OPN expression in HOS, U-87 MG and Caki-1 whole cell lysates. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from Worked well for IHC and WB with NTera2Worked well for IHC and WB with NTera2, 2102Ep and TCam-2 cell line. -SCBT Publication Review
Date published: 2015-01-29
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osteopontin/OPN/SPP1 抗体 (LFMb-14) is rated 4.7 out of 5 by 7.
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