OPLAH Antibody (E-6): sc-365027

OPLAH Antibody (E-6) is a mouse monoclonal IgG3 antibody that detects OPLAH in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). OPLAH, also known as 5-oxoprolinase (ATP-hydrolysing), plays a critical role in the gamma-glutamyl cycle, which is essential for the synthesis and metabolism of glutathione, a key antioxidant that regulates amino acid transport and protects cells from oxidative stress. The enzyme catalyzes the conversion of 5-oxo-L-proline to L-glutamate, a reaction that is coupled with the hydrolysis of ATP, highlighting its importance in energy metabolism and cellular function. OPLAH is a 1,288 amino acid protein that forms a homodimer and belongs to the oxoprolinase family, with highest expression levels found in the kidney, although also present in lower amounts in the lung, breast, colon, and ovary. The gene encoding OPLAH is located on human chromosome 8, which is associated with various diseases, including schizophrenia and congenital hypothyroidism, underscoring OPLAH′s potential relevance in both health and disease. Anti-OPLAH antibody (E-6) is an invaluable tool for researchers investigating the role of this enzyme in cellular processes and its implications in various pathologies.

The preferred OPLAH antibody is OPLAH Antibody (E-10): sc-271807.