OC-2 CRISPR/Cas9 KO Plasmid (m): sc-432551
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OC-2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OC-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OC-2 CRISPR/Cas9 KO Plasmid (m) | sc-432551 | 20 µg | $397.00 |
Overview
Onecut2 (OC-2) is a mouse homeobox transcription factor that coordinates cell fate specification and tissue patterning during development, with prominent roles in neurogenesis and organ differentiation. By binding regulatory DNA elements, OC-2 modulates transcriptional programs that intersect with lineage-determining networks controlling proliferation, differentiation, and regional identity. Onecut family activity contributes to maintenance of cellular identity and maturation in multiple epithelial and neuronal contexts. Dysregulated ONECUT2 expression has been associated with altered differentiation states and oncogenic transcriptional circuitry in several tumor types, supporting its utility as a mechanistic node in developmental and cancer biology studies.
OC-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Onecut2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Onecut2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Onecut2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OC-2 protein expression.
This CRISPR knockout system enables efficient generation of Onecut2-deficient cell models for investigation of OC-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Onecut2 exon(s) critical for OC-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Onecut2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OC-2 CRISPR/Cas9 KO Plasmid (m) and OC-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Onecut2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OC-2 HDR Plasmid (m) and OC-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Onecut2 homology arms to support homology-directed repair at defined Onecut2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.