Date published: 2025-9-6
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Nur77 Antibody (C-5) - Western Blotting - Image 356964
Nur77 抗体 (C-5) HRP: sc-365113 HRP. 非转染: sc-110760 (A) 和人 Nur77 转染: sc-112240 (B) 293 全细胞裂解液中 Nur77 表达的直接 Western 印迹分析.
Nur77 抗体 (C-5) HRP: sc-365113 HRP. 非转染: sc-110760 (A) 和人 Nur77 转染: sc-112240 (B) 293 全细胞裂解液中 Nur77 表达的直接 Western 印迹分析.

Nur77 抗体 (C-5): sc-365113

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说明书
  • Nur77 抗体 C-5 是小鼠单克隆 IgG1 κ，Nur77抗体, 在24篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Nur77的N-terminus的氨基酸4-31之间
  • Nur77 抗体 (C-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Nur77; 也和以下物种反应，包括： bovine
  • 抗Nur77抗体（C-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-365113 X, 200 µg/0.1 ml)
  • 关于如何获取Nur77 (C-5): sc-365113的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是Nur77 Antibody (C-5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Nur77 Antibody (C-5) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Nur77抗体（C-5）是一种IgG1 κ小鼠单克隆Nur77抗体（也称为NR4A1抗体或睾丸受体3抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Nur77蛋白。Nur77抗体（C-5）既有非偶联抗Nur77抗体形式，也有多种偶联形式的抗Nur77抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Nurr1（Nur相关因子1）和Nur77（也称为NGFI-B）编码孤儿核受体，这可能构成核受体超家族中的一个额外亚家族。小鼠Nurr1的大鼠和人类同源物分别命名为RNR1和NOT。Nurr1和Nur77都是生长因子可诱导的即早反应基因。在膜去极化后可见Nurr1和Nur77的诱导，而仅在NGF刺激下可见Nur77的诱导。JunD是Nur77的介导物。在G0到G1过渡期间以及整个G1阶段，活化的T细胞中Nur77表达增加。除了作为即早基因的功能外，Nur77还可能在TCR介导的凋亡中发挥作用。已显示强效免疫抑制剂环孢菌素A可抑制Nur77结合DNA的能力。Nur77的显性负性形式可以保护T细胞杂交瘤免受激活诱导的凋亡。然而，Nur77对TCR介导的凋亡的绝对要求仍存在争议。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nur77 抗体 (C-5) 参考文献:

  1. 鉴定新的脑特异性转录因子 NURR1。  |  Law, SW., et al. 1992. Mol Endocrinol. 6: 2129-35. PMID: 1491694
  2. 转化生长因子-β和 Nur77 在分化抑制因子 1 对结肠肿瘤发生的双重调控中的相互作用  |  Niu, B., et al. 2021. Nat Commun. 12: 2809. PMID: 33990575
  3. 发现5-(嘧啶-2-氨基)-1H-吲哚-2-甲酰胺衍生物作为Nur77调节剂，对三阴性乳腺癌具有选择性和强效活性。  |  Qin, J., et al. 2023. J Med Chem. 66: 15847-15866. PMID: 37983615
  4. Nur77的相分离通过促进Nur77/Bcl-2凝结体的形成，介导XS561诱导的细胞凋亡。  |  Chen, X., et al. 2024. Acta Pharm Sin B. 14: 1204-1221. PMID: 38486987
  5. Nur77通过调控miR-21a介导过敏性休克。  |  Jo, H., et al. 2024. Curr Issues Mol Biol. 46: 3175-3192. PMID: 38666929
  6. Nur77通过激活线粒体自噬和抑制凋亡来改善生殖衰老小鼠的卵巢功能。  |  Yao, Y., et al. 2024. Reprod Biol Endocrinol. 22: 86. PMID: 39044215
  7. 缺乏核受体 NGFI-B (Nur77) 的小鼠胸腺和外周 T 细胞死亡不受影响。  |  Lee, SL., et al. 1995. Science. 269: 532-5. PMID: 7624775
  8. NOT，一种与类固醇/甲状腺激素受体 NAK1/TR3 密切相关的人类即时早期反应基因。  |  Mages, HW., et al. 1994. Mol Endocrinol. 8: 1583-91. PMID: 7877627
  9. T 细胞活化过程中 NGFI-B 基因表达的诱导。蛋白磷酸酶的作用  |  Garcia, I., et al. 1994. J Immunol. 153: 3417-25. PMID: 7930567
  10. JunD 参与了 PC12 细胞中神经生长因子和膜去极化对孤儿受体基因 nur77 的转录激活。  |  Yoon, JK. and Lau, LF. 1994. Mol Cell Biol. 14: 7731-43. PMID: 7969116
  11. Nur77 孤儿类固醇受体在细胞凋亡中的分子特征。  |  Winoto, A. 1994. Int Arch Allergy Immunol. 105: 344-6. PMID: 7981602
  12. 肾上腺中孤儿核受体 Nur77 和 Nurr-1 的内分泌和神经源调控。  |  Davis, IJ. and Lau, LF. 1994. Mol Cell Biol. 14: 3469-83. PMID: 8164692

订购信息

产品名称产品编号规格价格数量收藏夹

Nur77 抗体 (C-5)

sc-365113
200 µg/ml
$316.00

Nur77 (C-5): m-IgG Fc BP-HRP 套装

sc-551924
200 µg Ab; 10 µg BP
$354.00

Nur77 抗体 (C-5) X

sc-365113 X
200 µg/0.1 ml
$316.00

Nur77 抗体 (C-5) AC

sc-365113 AC
500 µg/ml, 25% agarose
$416.00

Nur77 抗体 (C-5) HRP

sc-365113 HRP
200 µg/ml
$316.00

Nur77 抗体 (C-5) FITC

sc-365113 FITC
200 µg/ml
$330.00

Nur77 抗体 (C-5) PE

sc-365113 PE
200 µg/ml
$343.00

Nur77 抗体 (C-5) Alexa Fluor® 488

sc-365113 AF488
200 µg/ml
$357.00

Nur77 抗体 (C-5) Alexa Fluor® 546

sc-365113 AF546
200 µg/ml
$357.00

Nur77 抗体 (C-5) Alexa Fluor® 594

sc-365113 AF594
200 µg/ml
$357.00

Nur77 抗体 (C-5) Alexa Fluor® 647

sc-365113 AF647
200 µg/ml
$357.00

Nur77 抗体 (C-5) Alexa Fluor® 680

sc-365113 AF680
200 µg/ml
$357.00

Nur77 抗体 (C-5) Alexa Fluor® 790

sc-365113 AF790
200 µg/ml
$357.00

Nur77 (C-5) 中和勝肽

sc-365113 P
100 µg/0.5 ml
$68.00

I want a CHIPseq antibody for Nr4a1 in mice. I found that SC-5569 used to be used in mice ChIPseq but now discontinued, and the web page encouraged to use sc-365113. Do you have any information about quality of CHIP-seq?

Asked by: Hiroyuki
Thank you for your question. We have not validated the antibody for ChIPseq .
Answered by: BlakeJ
Date published: 2023-07-25

请问该抗体是否适用于小鼠组织冰冻切片荧光染色？

Asked by: ghwzzu
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Hello, is it possible to get a 10µg Gratis sample of Nur 77 (C-5): sc-35511 konjugatet with ether FITC or AF488? Many Thanks! Simone Kumstel

Asked by: SimoneKumstel
Thank you for your question. We do not provide sample sizes of any conjugated primary antibodies. This antibody is available for purchase conjugated to FITC or AF488. Please note that we do offer a 1-year product warranty from the date of shipment for the recommended applications stated on our website. If you require more information, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-26

WB做不出来，请问你们做出来的一抗稀释比例是多少

Asked by: wuxuan
感谢您的提问。推荐的稀释度是1:100-1:1000，关于此问题的详细细节，欢迎通过：电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. The recommended dilution for WB is 1:100-1:1000, for more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-02-01

What application is the blocking peptide sc-365113 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Santa的抗体非常好用Santa的抗体非常好用，特异性很好，跑出来的结果很清晰，而且价格也很优惠，售后也很棒，以后还会再买Santa的抗体。
Date published: 2025-04-17
Rated 5 out of 5 by from よく検出できました。2本抗体サンプルをもちいて、WB実験行いました。一つはよく検出できましたので、使用するようにしました。サンプルサイズは助かります。
Date published: 2021-06-09
Rated 5 out of 5 by from バンドが検出されます。WBで使用しました。非特異的なバンドが検出されますが問題ないと思います。目的のバンドはよりシャープなバンドとして検出されました。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 5 out of 5 by from Antibody validation in knockout mouseGreat antibody - we have confirm its specificity by IF comparing wild type to knockout neuronal tissues. Knockout mouse was purchased from Jackson Lab (Stock No: 006187). Antibody dilution 1/500 in BSA 1%.
Date published: 2017-09-20
Rated 5 out of 5 by from Good antibody!1:500 diluted for wb of rat protein ,the result is satisfied!
Date published: 2017-05-21
Rated 5 out of 5 by from Works really well for WBGives a nice single sharp band at around ~65 kD on Western blot. Tested with mouse brain (striatal) lysates.
Date published: 2017-03-15
Rated 5 out of 5 by from Great antibody for WesternsThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-17
Rated 5 out of 5 by from Good productThis antibody was good product. The signal was specific and strong. The dilution is 1:1000 in 5% BSA. I will use this antibody for IF next.
Date published: 2016-12-31
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Nur77 抗体 (C-5) is rated 5.0 out of 5 by 10.
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