Date published: 2025-9-7

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Nup93 抗体 (E-8): sc-374399

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说明书
  • Nup93 抗体 E-8 是小鼠单克隆 IgG1 κ,Nup93抗体, 在10篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的Nup93的N-terminus
  • Nup93 抗体 (E-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Nup93
  • 抗Nup93抗体(E-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Nup93 (E-8): sc-374399免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Nup93 Antibody (E-8) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Nup93 Antibody (E-8) 打包提供(请参阅下面的订购信息)。

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関連項目

Nup93 抗体(E-8)是一种 IgG1 κ 小鼠单克隆 Nup93 抗体(也称为 Nup93 抗体),可通过 WB、IP、IF、IHC(P) 和 ELISA 检测小鼠、大鼠和人类的 Nup93 蛋白。Nup93 抗体(E-8)既可以是非偶联的抗 Nup93 抗体形式,也可以是多种偶联形式的抗 Nup93 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。核孔复合物(NPC)介导真核细胞中核与细胞质之间的双向大分子运输,由 100 多个不同的亚基组成。其中许多亚基属于称为核孔蛋白(Nups)的家族,其特征是存在 O-连接-N-乙酰葡糖胺部分和独特的五肽重复序列(XFXFG)。Nup93(核孔蛋白 93)是每个 NPC 中发现的最丰富的核孔蛋白,占总质量的 10% 以上。它主要定位于 NPC 的核侧,特别是在篮状终端环区域,并与 Nup188、Nup53 和 Nup205 形成复合物。该复合物对于 NPC 的稳定性和正确组装至关重要。Nup93 直接与位于 NPC 中心的 Nup62 复合物相互作用,从而将这两个亚复合物连接在一起。Nup93 由其 N 端的卷曲螺旋结构域和 C 端螺旋结构域组成。其正常功能对于细胞活力和正常 NPC 功能至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nup93 抗体 (E-8) 参考文献:

  1. Nup192p 是一种保守的核蛋白,优先位于核膜内侧。  |  Kosova, B., et al. 1999. J Biol Chem. 274: 22646-51. PMID: 10428845
  2. 与 nup153 的直接相互作用介导了 Tpr 与核孔复合体外围的结合。  |  Hase, ME. and Cordes, VC. 2003. Mol Biol Cell. 14: 1923-40. PMID: 12802065
  3. 秀丽隐杆线虫核蛋白 Nup93 和 Nup205 决定了体内核孔复合体大小排斥的极限。  |  Galy, V., et al. 2003. Mol Biol Cell. 14: 5104-15. PMID: 12937276
  4. 核蛋白是核孔复合体核心结构的组成部分,Tpr是核篮子的结构元素。  |  Krull, S., et al. 2004. Mol Biol Cell. 15: 4261-77. PMID: 15229283
  5. 脊椎动物 Nup53 与核薄层相互作用,是组装含 Nup93 的复合物所必需的。  |  Hawryluk-Gara, LA., et al. 2005. Mol Biol Cell. 16: 2382-94. PMID: 15703211
  6. 分子克隆出主要在性腺和肾脏中表达的稻田鳗鲡 Nup93。  |  Shang, X., et al. 2006. Yi Chuan Xue Bao. 33: 41-8. PMID: 16450586
  7. CREB 结合蛋白(CBP)与核转运蛋白的功能相互作用以及 HDAC 抑制剂的调节作用。  |  Ryan, CM., et al. 2006. Cell Cycle. 5: 2146-52. PMID: 16969114
  8. 核蛋白 Nic96 的晶体结构揭示了一种新颖, 复杂的螺旋结构域。  |  Jeudy, S. and Schwartz, TU. 2007. J Biol Chem. 282: 34904-12. PMID: 17897938
  9. 核孔基因 NUP93, NUP205 和 XPO5 突变导致类固醇耐受性肾病综合征。  |  Braun, DA., et al. 2016. Nat Genet. 48: 457-65. PMID: 26878725
  10. 通过异常调控基因转录,下调 NUP93 会加重缺氧诱导的体外心肌细胞死亡。  |  Pan, L., et al. 2023. Acta Pharmacol Sin. 44: 969-983. PMID: 36807413
  11. 转录因子阴阳1增强核蛋白93癌基因的活性并调节膀胱癌的恶性程度。  |  Zhang, G., et al. 2024. Toxicol In Vitro. 99: 105875. PMID: 38857852
  12. Nup93是酵母Nic96p的脊椎动物同源物,它与一种新型205-kDa蛋白形成复合物,是正确组装核孔所必需的。  |  Grandi, P., et al. 1997. Mol Biol Cell. 8: 2017-38. PMID: 9348540

订购信息

产品名称产品编号规格价格数量收藏夹

Nup93 抗体 (E-8)

sc-374399
200 µg/ml
$316.00

Nup93 (E-8): m-IgG Fc BP-HRP 套装

sc-529735
200 µg Ab; 10 µg BP
$354.00

Nup93 (E-8): m-IgGκ BP-HRP 套装

sc-522723
200 µg Ab, 40 µg BP
$354.00

Nup93 (E-8): m-IgG1 BP-HRP 套装

sc-543660
200 µg Ab; 20 µg BP
$354.00

Nup93 抗体 (E-8) AC

sc-374399 AC
500 µg/ml, 25% agarose
$416.00

Nup93 抗体 (E-8) HRP

sc-374399 HRP
200 µg/ml
$316.00

Nup93 抗体 (E-8) FITC

sc-374399 FITC
200 µg/ml
$330.00

Nup93 抗体 (E-8) PE

sc-374399 PE
200 µg/ml
$343.00

Nup93 抗体 (E-8) Alexa Fluor® 488

sc-374399 AF488
200 µg/ml
$357.00

Nup93 抗体 (E-8) Alexa Fluor® 546

sc-374399 AF546
200 µg/ml
$357.00

Nup93 抗体 (E-8) Alexa Fluor® 594

sc-374399 AF594
200 µg/ml
$357.00

Nup93 抗体 (E-8) Alexa Fluor® 647

sc-374399 AF647
200 µg/ml
$357.00

Nup93 抗体 (E-8) Alexa Fluor® 680

sc-374399 AF680
200 µg/ml
$357.00

Nup93 抗体 (E-8) Alexa Fluor® 790

sc-374399 AF790
200 µg/ml
$357.00

Can Nup93 (E-8): sc-374399 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, Nup93 (E-8): sc-374399 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24

この抗体を注文予定のものですが、すごくきれいに免疫染色でも染まっていますが、 パラフィン切片での染色(蛍光抗体法)を検討中なのですが、抗原賦活の方法や、具体的な染色手順を ご教示いただけませんでしょうか。よろしくお願いいたいします。

Asked by: shiroko
Thank you for your question. For detailed protocol information, please refer to our online protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining お問い合わせを頂きまして、誠にありがとうございました。 プロトコルの参考については、こちらをご覧ください: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 5
Date published: 2017-02-10
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Rated 5 out of 5 by from Nice antibody against Nup93This antibody at 1:200 dilution can detect a very specific band.
Date published: 2017-11-21
Rated 4 out of 5 by from nice staining in IF assayI got the free trial and it showed good staining in the cells.
Date published: 2017-10-12
Rated 5 out of 5 by from Excellent for WBit works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
Rated 5 out of 5 by from Good for multiple applicationsStrong antibody performance in Western Blot, IF and IHC!
Date published: 2017-01-17
Rated 5 out of 5 by from Great production!The antibody works well in western blot and immunofluorescence.
Date published: 2016-11-02
Rated 5 out of 5 by from Antibody is working well no problemsAntibody is working well no problems.
Date published: 2015-08-26
Rated 5 out of 5 by from Produced superb immunoperoxidase nuclearProduced superb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-04-28
Rated 5 out of 5 by from Good specificity in WB in 293TGood specificity in WB in 293T, T24, LADMAQC and MTE1D whole cell lysates. -SCBT QC
Date published: 2015-04-16
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Nup93 抗体 (E-8) is rated 4.9 out of 5 by 8.
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