Date published: 2025-9-8

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NOS2/iNOS 抗体 (C-11): sc-7271

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说明书
  • NOS2/iNOS 抗体 C-11 是小鼠单克隆 IgG1 κ,NOS2/iNOS抗体, 在635篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于mouse物种的NOS2的C-terminus附近的氨基酸1126-1144之间
  • NOS2/iNOS 抗体 (C-11) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA,检测mouse, rat 和human 来源的 NOS2 (iNOS)
  • 抗NOS2/iNOS抗体(C-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取NOS2/iNOS (C-11): sc-7271免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 NOS2/iNOS 抗体 (C-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NOS2/iNOS 抗体 (C-11) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

NOS2抗体(C-11)是一种小鼠单克隆IgG1 kappa轻链抗体,用于检测一氧化氮合酶2(NOS2),也称为诱导型一氧化氮合酶(iNOS)或NOS2A。NOS2小鼠单克隆抗体(C-11)靶向小鼠NOS2蛋白C端附近1126-1144氨基酸之间的表位,确保精确结合和可靠检测。NOS2是一种关键酶,负责一氧化氮(NO)的合成,一氧化氮是一种多功能信号分子,参与免疫反应、炎症和神经传递等多种生物过程。NOS2在宿主防御机制中起着特别重要的作用,它产生大量一氧化氮来消灭病原体,同时调节炎症通路。NOS2小鼠单克隆抗体(C-11)对多种物种均具有广泛的反应性,可识别小鼠、大鼠和人类的NOS2,推荐用于包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)、流式细胞术(FCM)和酶联免疫吸附测定(ELISA)在内的应用。此外,NOS2单克隆抗体(C-11)有非结合型和多种结合型,如琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物,为不同的实验设置提供了灵活性。NOS2抗体(C-11)推荐用于研究一氧化氮在健康和疾病中的作用的研究人员,有助于加深对炎症反应和潜在治疗靶点的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NOS2/iNOS 抗体 (C-11) 参考文献:

  1. 一氧化氮的上皮自体毒性:在百日咳呼吸道细胞病理学中的作用。  |  Heiss, LN., et al. 1994. Proc Natl Acad Sci U S A. 91: 267-70. PMID: 7506415
  2. 巨噬细胞中 NO 合酶的诱导需要转录因子 IRF-1。  |  Kamijo, R., et al. 1994. Science. 263: 1612-5. PMID: 7510419
  3. 一氧化氮合酶:作用, 收费和控制。  |  Nathan, C. and Xie, QW. 1994. Cell. 78: 915-8. PMID: 7522969
  4. 一氧化氮合酶:结构和催化方面的问题。  |  Marletta, MA. 1994. Cell. 78: 927-30. PMID: 7522970
  5. 人类免疫缺陷病毒 1 型(HIV-1)感染的单核细胞中一氧化氮合酶活性的调节:对 HIV 相关神经疾病的影响。  |  Bukrinsky, MI., et al. 1995. J Exp Med. 181: 735-45. PMID: 7530762
  6. 工作中没有。  |  Schmidt, HH. and Walter, U. 1994. Cell. 78: 919-25. PMID: 7923361
  7. 一氧化氮是体内杀瘤活性的重要介质。  |  Farias-Eisner, R., et al. 1994. Proc Natl Acad Sci U S A. 91: 9407-11. PMID: 7937779

订购信息

产品名称产品编号规格价格数量收藏夹

NOS2/iNOS 抗体 (C-11)

sc-7271
200 µg/ml
$316.00

NOS2/iNOS (C-11): m-IgG Fc BP-HRP 套装

sc-526492
200 µg Ab; 10 µg BP
$354.00

NOS2/iNOS (C-11): m-IgG1 BP-HRP 套装

sc-531865
200 µg Ab; 20 µg BP
$354.00

NOS2/iNOS 抗体 (C-11) AC

sc-7271 AC
500 µg/ml, 25% agarose
$416.00

NOS2/iNOS 抗体 (C-11) HRP

sc-7271 HRP
200 µg/ml
$316.00

NOS2/iNOS 抗体 (C-11) FITC

sc-7271 FITC
200 µg/ml
$330.00

NOS2/iNOS 抗体 (C-11) PE

sc-7271 PE
200 µg/ml
$343.00

NOS2/iNOS 抗体 (C-11) Alexa Fluor® 488

sc-7271 AF488
200 µg/ml
$357.00

NOS2/iNOS 抗体 (C-11) Alexa Fluor® 546

sc-7271 AF546
200 µg/ml
$357.00

NOS2/iNOS 抗体 (C-11) Alexa Fluor® 594

sc-7271 AF594
200 µg/ml
$357.00

NOS2/iNOS 抗体 (C-11) Alexa Fluor® 647

sc-7271 AF647
200 µg/ml
$357.00

NOS2/iNOS 抗体 (C-11) Alexa Fluor® 680

sc-7271 AF680
200 µg/ml
$357.00

NOS2/iNOS 抗体 (C-11) Alexa Fluor® 790

sc-7271 AF790
200 µg/ml
$357.00

NOS2/iNOS 抗体 (C-11) TRITC

sc-7271 TRITC
200 µg/ml
$320.00

NOS2/iNOS 抗体 (C-11) Alexa Fluor® 405

sc-7271 AF405
200 µg/ml
$357.00

NOS2/iNOS (C-11) 中和勝肽

sc-7271 P
100 µg/0.5 ml
$68.00

Nos2内皮功能是什么

Asked by: Season
感谢您的提问。请联系我们的技术支持部门了解更多信息。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 12
Date published: 2021-11-25

Is this antibody possible for iNOS detection using IHC-F staning?

Asked by: yueyao
Thank you for your question. Anti-NOS2 Antibody (C-11): sc-7271 is suited for immunohistochemistry in Frozen Tissue Sections and Formalin-Fixed, Paraffin-Embedded Tissue Sections.
Answered by: Tech Support Europe
Date published: 2021-07-30

This NOS2 antibody is a mouse antibody, and it's also recommended for mouse IHC detection, the question is what secondary antibody should I choose for the detection?

Asked by: phhsy
Thank you for your question. You can use any anti-mouse secondary antibody for your IHC on mouse samples. Make sure to use a good blocking reagent or blocking serum before adding antibodies.
Answered by: Tech Service
Date published: 2019-10-25

The host species is mouse, correct?

Asked by: Conner
Yes, this antibody is raised in a mouse.
Answered by: Technical Support
Date published: 2018-12-21

Hi, can this antibody be use on rat's retina in iNOS detection using IHC staning?

Asked by: Fatt
Thank you for your question. Yes, this antibody is suitable for detection of iNOS, also designated NOS2, in rat tissues. We are aware of publications citing use of this antibody in retina tissue specifically. For example, PMID's: 21712024 and 20621070.
Answered by: Technical Support
Date published: 2023-08-11

How specific is NOS2 (c-11) for Rat. I would like to try it for iNOS detection in rat brain tissue. Please provide me any information related to this. I will highly appreciate your quickest response.

Asked by: Golam Mustafa
Thank you for your question.This antibody is very good for use on rat samples, we have many publications on rat samples, including rat neuronal tissue. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-06-28

Welcher Clon verbirgt sich hinter dem Antikörper NOS2 (C-11) (Bestell-Nr.: sc-7271 AF647)?

Asked by: Ahornblatt5
Vielen Dank für Ihre Anfrage und Ihr Interesse an diesem Produkt. Der NOS2 Antikörper (C-11): sc-7271 ist ein hauseigenes Produkt. Unsere interne Bezeichnung für diesen Klon ist C-11 wie im Produktnamen innerhalb der Klammern angegeben. Für weitere Informationen wenden Sie sich bitte an unseren Technischen Service in unserem Europäischen Supportbüro in Heidelberg. Sie erreichen uns telefonisch unter 06221 4503 0, per email an: europe@scbt.com oder per Live-Chat auf unserer Webseite www.scbt.com Wir freuen uns Ihnen weiterzuhelfen.
Answered by: Technical Support Europe
Date published: 2017-05-16

Dose it work well in rat brain tissue for IF?

Asked by: soahmen
Thank you for your question. Yes, this antibody should be fine for rat brain samples on IF applications. It is a very strong antibody that has been published previously in rat brain samples. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-02-23
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Rated 5 out of 5 by from ExcellentTo be honest, everyone knows that Santa's antibody didn't work sometimes. In my IFC experiment, all cells turned red, and although the control group showed a lower signal than the inflammation group, I was still concerned about whether it was non-specific binding. However, after introducing an LPS-treated positive control group, the results became highly specific. This made me realize that the antibody is actually useful. It seems that the inflammation inducer I used might have behaved a bit unusually, but the antibody itself performs well.
Date published: 2025-06-06
Rated 5 out of 5 by from GoodGreat antibody for WB, works in mouse RAW 264.7 cells
Date published: 2025-03-18
Rated 1 out of 5 by from Did not workDid not work. It just did not work/do what it was supposed to do
Date published: 2025-02-26
Rated 5 out of 5 by from Great features for western blotThe antibody can give a nice clean band. It is a good antibody.
Date published: 2024-07-03
Rated 5 out of 5 by from Expression for oxidative stressNOS2 expression showing presence of inflammation at the defect site in a osteoporotic infectious hip fracture study
Date published: 2024-06-12
Rated 5 out of 5 by from The antibody was greatFew months ago, we determined the iNOS level in colon tissues of mice. The band was clear and precise.
Date published: 2024-01-02
Rated 5 out of 5 by from opinions about antibodiesvery good antibodies, high detection, work very well with the highest dilution declared on the leaflet
Date published: 2023-04-16
Rated 5 out of 5 by from Clean and specific to NOS2Clean and specific antibody. Tested on western blot analysis
Date published: 2023-01-25
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NOS2/iNOS 抗体 (C-11) is rated 4.1 out of 5 by 69.
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