I am using Nitrotyrosine (7A5): sc-81482 antibody for immunoprecipitation, how much antibody should I use?
Asked by: Randy McDonald
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here:
https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots