Nidogen Antibody (JF3): sc-57002
- Nidogen Antibody (JF3) is a rat monoclonal IgG2a provided at 200 µg/ml
- raised against recombinant fragment corresponding to the G1/Link region of Nidogen of mouse origin
- recommended for detection of Nidogen of mouse, rat and human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nidogen Antibody (JF3). This work is in progress.
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Nidogen Antibody (JF3) is a rat monoclonal IgG2a antibody that detects Nidogen in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. Nidogen, also known as Entactin, is a crucial sulfated glycoprotein that plays a significant role in the formation and stability of basement membranes, which are essential for tissue architecture and cellular organization. Anti-Nidogen antibody (JF3) recognizes a vital linker between key extracellular matrix components, specifically Laminin 1 and Collagen Type IV, facilitating their assembly into a functional matrix. Nidogen′s importance extends beyond structural support; this glycoprotein is involved in regulating cellular adhesion and signaling processes, particularly in mesenchymal peritubular and myoepithelial cells, where Nidogen influences cell behavior in an autocrine manner. Notably, Nidogen is highly conserved across species, and Nidogen expression is tightly regulated during embryonic and fetal development, being present exclusively in fully developed ectodermal basement membranes. This specificity underscores Nidogen′s critical role in developmental biology and tissue integrity, making Nidogen (JF3) monoclonal antibody an invaluable tool for researchers studying extracellular matrix dynamics and related cellular processes.
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Nidogen Antibody (JF3) References:
- Crystal structure and mutational analysis of a perlecan-binding fragment of nidogen-1. | Hopf, M., et al. 2001. Nat Struct Biol. 8: 634-40. PMID: 11427896
- Specific ablation of the nidogen-binding site in the laminin gamma1 chain interferes with kidney and lung development. | Willem, M., et al. 2002. Development. 129: 2711-22. PMID: 12015298
- Basement-membrane heparan sulfate proteoglycan binds to laminin by its heparan sulfate chains and to nidogen by sites in the protein core. | Battaglia, C., et al. 1992. Eur J Biochem. 208: 359-66. PMID: 1521532
- Compound genetic ablation of nidogen 1 and 2 causes basement membrane defects and perinatal lethality in mice. | Bader, BL., et al. 2005. Mol Cell Biol. 25: 6846-56. PMID: 16024816
- Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo. | Lee, HK., et al. 2006. FEBS Lett. 580: 2253-7. PMID: 16574105
- Acute changes of nidogen immunoreactivity in the basal lamina of the spinal cord vessels following dorsal hemisection without correlative changes of nidogen gene expression. | Ae Seo, I., et al. 2007. Acta Histochem. 109: 446-53. PMID: 17570475
- Structure and function of the laminin-nidogen complex. | Timpl, R., et al. 1990. Ann N Y Acad Sci. 580: 311-23. PMID: 2110795
- The growth factor-like adipokine tartrate-resistant acid phosphatase 5a interacts with the rod G3 domain of adipocyte-produced nidogen-2. | Patlaka, C., et al. 2014. Biochem Biophys Res Commun. 454: 446-52. PMID: 25450682
- Laminin-nidogen complex. Extraction with chelating agents and structural characterization. | Paulsson, M., et al. 1987. Eur J Biochem. 166: 11-9. PMID: 3109910
- Identification and interaction repertoire of large forms of the basement membrane protein nidogen. | Dziadek, M., et al. 1985. EMBO J. 4: 2513-8. PMID: 3932063
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nidogen Antibody (JF3) | sc-57002 | 200 µg/ml | $322.00 |