Date published: 2025-9-9
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Neutrophil Elastase Antibody (G-2) - Western Blotting - Image 14632
中性粒细胞弹性蛋白酶抗体 (G-2): sc-55549. U-937 (A) 和 HL-60 (B) 全细胞裂解液中中性粒细胞弹性蛋白酶表达的 Western 印迹分析.
中性粒细胞弹性蛋白酶抗体 (G-2): sc-55549. U-937 (A) 和 HL-60 (B) 全细胞裂解液中中性粒细胞弹性蛋白酶表达的 Western 印迹分析.

Neutrophil Elastase 抗体 (G-2): sc-55549

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说明书
  • Neutrophil Elastase 抗体 G-2 是小鼠单克隆 IgG1 κ，Neutrophil Elastase抗体, 在33篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Neutrophil Elastase的氨基酸211-267
  • Neutrophil Elastase 抗体 (G-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Neutrophil Elastase
  • 抗Neutrophil Elastase抗体（G-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取Neutrophil Elastase (G-2): sc-55549的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Neutrophil Elastase 抗体 (G-2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Neutrophil Elastase 抗体包括 Neutrophil Marker

中性粒细胞弹性蛋白酶抗体（G-2）是一种小鼠单克隆 IgG1 kappa 轻链抗体，设计用于识别人中性粒细胞弹性蛋白酶（NE）（又称 ELANE 或 medullasin）的 211-267 氨基酸。抗 NE 抗体 (G-2) 可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附试验（ELISA）等应用有效检测 NE，因此是各种研究环境中必不可少的工具。NE 是一种丝氨酸蛋白酶，主要储存在中性粒细胞的嗜氮颗粒中，在免疫反应过程中，NE 在降解吞噬的病原体和重塑细胞外基质方面发挥着关键作用。NE 的翻译后修饰（包括糖基化）对其正常折叠、稳定性和酶活性至关重要，可确保其有效发挥作用，同时不会对组织造成意外损害。这些修饰还能调节 NE 与补体系统中其他蛋白质的相互作用，以及作为血小板激动剂的作用，从而增强血小板的聚集、分泌和钙动员。这些修饰所保持的结构完整性对于 NE 参与特发性肺纤维化、类风湿性关节炎、成人呼吸窘迫综合征和囊性纤维化等炎症性疾病至关重要。NE 单克隆抗体 (G-2) 可特异性地靶向 NE 的修饰区域，提供可靠而准确的检测，有助于对炎症和组织损伤的分子机制进行深入研究。NE (G-2) 抗体既有非共轭形式，也有多种共轭形式，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Neutrophil Elastase 抗体 (G-2) 参考文献:

  1. 弹性蛋白酶和金属蛋白酶活性可调节可溶性补体受体 1 的释放。  |  Sadallah, S., et al. 1999. Eur J Immunol. 29: 3754-61. PMID: 10556832
  2. 中性粒细胞弹性蛋白酶能增强凝血酶 G 诱导的血小板活化。  |  Selak, MA. 1992. Thromb Haemost. 68: 570-6. PMID: 1333649
  3. 人中性粒细胞弹性蛋白酶的分子克隆。  |  Farley, D., et al. 1988. Biol Chem Hoppe Seyler. 369 Suppl: 3-7. PMID: 2462434
  4. 使用与常规活检样本反应的单克隆抗中性粒细胞弹性蛋白酶（NP57）诊断急性髓性白血病。  |  Ralfkiaer, E., et al. 1989. Histopathology. 14: 637-43. PMID: 2759560
  5. 在正常和白血病骨髓细胞中使用人类中性粒细胞弹性蛋白酶单克隆抗体。  |  Pulford, KA., et al. 1988. J Clin Pathol. 41: 853-60. PMID: 2844860
  6. 中性粒细胞弹性蛋白酶在慢性炎症中的作用  |  Döring, G. 1994. Am J Respir Crit Care Med. 150: S114-7. PMID: 7952645
  7. 炎症中中性粒细胞与血小板的相互作用  |  Selak, MA. 1994. Receptor. 4: 3-7. PMID: 8038704
  8. 中性粒细胞弹性蛋白酶会增加气道上皮细胞中分泌型白细胞蛋白酶抑制剂转录本的水平。  |  Abbinante-Nissen, JM., et al. 1993. Am J Physiol. 265: L286-92. PMID: 8105697
  9. 凝血酶 G 和凝血酶：两种不同血小板受体的证据。  |  Selak, MA. 1994. Biochem J. 297 (Pt 2): 269-75. PMID: 8297330

订购信息

产品名称产品编号规格价格数量收藏夹

Neutrophil Elastase 抗体 (G-2)

sc-55549
200 µg/ml
$316.00

Neutrophil Elastase 抗体 (G-2) AC

sc-55549 AC
500 µg/ml, 25% agarose
$416.00

Neutrophil Elastase 抗体 (G-2) HRP

sc-55549 HRP
200 µg/ml
$316.00

Neutrophil Elastase 抗体 (G-2) FITC

sc-55549 FITC
200 µg/ml
$330.00

Neutrophil Elastase 抗体 (G-2) PE

sc-55549 PE
200 µg/ml
$343.00

Neutrophil Elastase 抗体 (G-2) Alexa Fluor® 488

sc-55549 AF488
200 µg/ml
$357.00

Neutrophil Elastase 抗体 (G-2) Alexa Fluor® 546

sc-55549 AF546
200 µg/ml
$357.00

Neutrophil Elastase 抗体 (G-2) Alexa Fluor® 594

sc-55549 AF594
200 µg/ml
$357.00

Neutrophil Elastase 抗体 (G-2) Alexa Fluor® 647

sc-55549 AF647
200 µg/ml
$357.00

Neutrophil Elastase 抗体 (G-2) Alexa Fluor® 680

sc-55549 AF680
200 µg/ml
$357.00

Neutrophil Elastase 抗体 (G-2) Alexa Fluor® 790

sc-55549 AF790
200 µg/ml
$357.00

Neutrophil Elastase 抗体 (G-2) B

sc-55549 B
200 µg/ml
$320.00

Hello there, I need mouse Neutrophil Elastase Antibody Alexa Fluor® 647 for imaging flow cytometry. Kindly give me a link. Thank you. Adekunle

Asked by: Adekunle
Thank you for contacting Santa Cruz Biotechnology. Please scroll to the bottom of the webpage to see the conjugated forms : https://www.scbt.com/p/neutrophil-elastase-antibody-g-2 https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2025-07-28

Which antigen retrival method you tried with that specific antibody? Did it work with Tris-EDTA pH 9.0?

Asked by: Soff00
Thank you for your question. We recommend performing antigen retrieval with sodium citrate, with heat. You can find the specifics in our protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-08-14

How can Neutrophil Elastase (G-2): sc-55549 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: cjMara
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody, Neutrophil Elastase (G-2), is currently available conjugated to phycoerythrin (sc-55549 PE), fluorescein (sc-55549 FITC), Alexa Fluor® 488 (sc-55549 AF488) or Alexa Fluor® 647 (sc-55549 AF647)
Answered by: Technical Service 11
Date published: 2017-01-22

Can you recommend a positive control to use with Neutrophil Elastase (G-2): sc-55549 monoclonal antibody for Western blot?

Asked by: jenniferc
We recommend U-937 (sc-2239) or HL-60 (sc-2209) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of Neutrophil Elastase in these cell lines.
Answered by: Technical Support 9
Date published: 2017-01-11
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Rated 5 out of 5 by from good antibody recognise human and mouse neutrophilNeutrophil Elastase expressed in blood film of transgenic mouse suffered from systemic inflammation. Neutrophil Elastase expressed in the activated neutrophil in the lysosomes. Azurophil granules stained with alexa 488. Very good antibody recognise human and mouse neutrophil.
Date published: 2017-02-17
Rated 5 out of 5 by from Superb !!Oustanding western blot data; specific band; also strong IHC
Date published: 2017-01-29
Rated 1 out of 5 by from IHC (P): no staining of neutrophilsThe antibody does not react with neutrophils in normal human spleen paraffin sections (fixation by 3.7% formol in tap water for 24h). Antigen retrievals tested were: 1) autoclaving in citrate buffer pH 6.0; 2) protease XIV; 3) no pretreatment.
Date published: 2017-01-18
Rated 5 out of 5 by from Excellent antibodyThe Neutrophil Elastase Antibody (G-2): sc-55549 works very well for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited several times for further verification.
Date published: 2017-01-12
Rated 5 out of 5 by from Used for immunohistochemical analysis of plasmaUsed for immunohistochemical analysis of plasma, ascitic fluid, and pancreas sections from male wistar rats. -SCBT Publication Review
Date published: 2015-04-24
Rated 5 out of 5 by from Robust bands observed at the correct molecularRobust bands observed at the correct molecular weight in western blot analysis of Neutrophil Elastase expression in U-937 and HL-60 whole cell lysates. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Immunofluorescence with pfa fixed rat brainImmunofluorescence with pfa fixed rat brain sections. -SCBT Publication Review
Date published: 2015-02-23
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic stainingSuperb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human bone marrow tissue. -SCBT QC
Date published: 2015-01-17
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Neutrophil Elastase 抗体 (G-2) is rated 4.5 out of 5 by 8.
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