Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Neurensin-1 CRISPR/Cas9 KO Plasmid (m): sc-423681
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neurensin-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Neurensin-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Neurensin-1 Antibody (22): sc-136360
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neurensin-1 CRISPR/Cas9 KO Plasmid (m) | sc-423681 | 20 µg | $397.00 |
Overview
Nrsn1 encodes Neurensin-1, a neuron-enriched membrane-associated protein implicated in endosomal trafficking and organization of vesicular compartments within neurites. Neurensin-1 has been linked to processes that support synaptic development and maintenance, including regulation of cargo sorting and dynamics of intracellular membranes important for neuronal connectivity. Altered Nrsn1 expression or network context has been associated with neurodevelopmental and neuropsychiatric phenotypes in mouse and human genetic studies, highlighting its relevance to circuits governing behavior. As a marker and modulator of neuronal membrane trafficking, Neurensin-1 is useful for investigating pathways that couple endosome function to synaptic structure and neuronal homeostasis.
Neurensin-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nrsn1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nrsn1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nrsn1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Neurensin-1 protein expression.
This CRISPR knockout system enables efficient generation of Nrsn1-deficient cell models for investigation of Neurensin-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nrsn1 exon(s) critical for Neurensin-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nrsn1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Neurensin-1 CRISPR/Cas9 KO Plasmid (m) and Neurensin-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nrsn1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Neurensin-1 HDR Plasmid (m) and Neurensin-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nrsn1 homology arms to support homology-directed repair at defined Nrsn1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.