Date published: 2025-9-9
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NDR1 Antibody (YJ-7) - Western Blotting - Image 148659
NDR1 抗体 (YJ-7): sc-100404. U-937 (A) 和 HEL 92. 1. 7 (B) 核提取物以及人 PBL 全细胞裂解液 (C) 中 NDR1 表达的 Western 印迹分析.
NDR1 抗体 (YJ-7): sc-100404. U-937 (A) 和 HEL 92. 1. 7 (B) 核提取物以及人 PBL 全细胞裂解液 (C) 中 NDR1 表达的 Western 印迹分析.

NDR1 抗体 (YJ-7): sc-100404

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说明书
  • NDR1抗体(YJ-7)是小鼠单克隆IgG2a κ, 在4篇文献中引用，规格为50 µg/0.5 ml
  • 免疫human物种的重组NDR1
  • 推荐用于 mouse, rat 和 human 来源的NDR1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取NDR1 (YJ-7): sc-100404的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 NDR1 抗体 (YJ-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NDR1 抗体 (YJ-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 NDR 抗体 包括 NDR2

NDR1抗体（YJ-7）是一种IgG2a κ小鼠单克隆NDR1抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的NDR1。NDR1抗体（YJ-7）为非偶联的抗NDR1抗体形式。核Dbf2相关激酶（NDR1和NDR2）参与细胞分裂和形态的调节，可能与肿瘤进展有关。NDR1和NDR2的氨基酸序列有86%的相似性，但它们的表达模式不同。NDR1定位于细胞核，而NDR2则呈点状细胞质分布。此外，NDR1在脾、肺和胸腺中的表达量最高，而NDR2在胃肠道中的表达量最高。然而，NDR1和NDR2都受磷酸化和Ca2+-结合蛋白S-100B的调节。NDR1和NDR2也可能在HIV-1生命周期中发挥作用。这两种蛋白都被HIV-1蛋白酶（PR）裂解，从而抑制它们的酶活性并改变NDR2的亚细胞定位。编码人类NDR1和NDR2的基因分别定位于染色体6p21和12p11.23。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NDR1 抗体 (YJ-7) 参考文献:

  1. Ca2+ 通过自身磷酸化和上游激酶的磷酸化介导 NDR 蛋白激酶的调节机制。  |  Tamaskovic, R., et al. 2003. J Biol Chem. 278: 6710-8. PMID: 12493777
  2. 多位点磷酸化和 S100B 钙结合蛋白对 NDR2 蛋白激酶的调控。  |  Stegert, MR., et al. 2004. J Biol Chem. 279: 23806-12. PMID: 15037617
  3. 人类 Mob 蛋白调控 NDR1 和 NDR2 丝氨酸-苏氨酸激酶。  |  Devroe, E., et al. 2004. J Biol Chem. 279: 24444-51. PMID: 15067004
  4. hMOB1 蛋白激活 NDR（核 Dbf2 相关）蛋白激酶的机制。  |  Bichsel, SJ., et al. 2004. J Biol Chem. 279: 35228-35. PMID: 15197186
  5. 对机构收容的智障成人中的弓形虫和强直虫进行血清流行病学研究。  |  Huminer, D., et al. 1992. Am J Trop Med Hyg. 46: 278-81. PMID: 1558266

订购信息

产品名称产品编号规格价格数量收藏夹

NDR1 抗体 (YJ-7)

sc-100404
50 µg/0.5 ml
$333.00

NDR1 (YJ-7): m-IgG Fc BP-HRP 套装

sc-539688
50 µg Ab; 10 µg BP
$354.00

NDR1 (YJ-7): m-IgG2a BP-HRP 套装

sc-546752
50 µg Ab; 10 µg BP
$354.00

Does this antibody recognize only Ndr1 (Stk38) or also Ndr2 (Stk38L)?

Asked by: Helene Leger
Thank you for your question . sc-100404 is not reactive to Ndr2.
Answered by: BlakeJ
Date published: 2023-02-15

Can sc-100404: NDR1 (YJ-7) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, sc-100404: NDR1 (YJ-7) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from 免疫印迹看评论似乎这个货号比较好，但我们的实验结果却相反，我们推荐sc-365555用于做western blotting。
Date published: 2020-10-01
Rated 4 out of 5 by from ottimo funzionamento per WBottimo funzionamento per WB su estratto cellule HeLa
Date published: 2019-04-04
Rated 5 out of 5 by from Excellent choice for NDR1!Well-rounded antibody that gives great results in all the tested applications, IHC, WB and IF.
Date published: 2017-02-08
Rated 4 out of 5 by from Strong band in WB in HeLa nuclear extractStrong band in WB in HeLa nuclear extract. -SCBT QC
Date published: 2015-05-16
Rated 4 out of 5 by from Produced clear immunofluorescence nuclearProduced clear immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-15
Rated 4 out of 5 by from Strong signal strength in WB in UStrong signal strength in WB in U-937 and HEL 92.1.7 nuclear extracts and human PBL whole cell lysate. -SCBT QC
Date published: 2015-02-11
Rated 4 out of 5 by from Good specificity in WB in PCGood specificity in WB in PC-12 and RAW264.7 whole cell lysates. -SCBT QC
Date published: 2015-01-22
Rated 4 out of 5 by from Robust signal in WB in Jurkat nuclear extractRobust signal in WB in Jurkat nuclear extract. -SCBT QC
Date published: 2015-01-08
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NDR1 抗体 (YJ-7) is rated 4.0 out of 5 by 8.
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