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Nanog 抗体 (C-4): sc-376915

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说明书
  • Nanog 抗体 C-4 是小鼠单克隆 IgG1 κ,Nanog抗体, 在30篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列181-329位于mouse物种的Nanog的C-terminus
  • Nanog 抗体 (C-4) 推荐用于WB, IP, IF 和 ELISA,检测mouse 来源的 Nanog
  • 抗Nanog抗体(C-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Nanog (C-4): sc-376915免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Nanog Antibody (C-4) for WB applications. 的首选辅助检测试剂,这些试剂现与Nanog Antibody (C-4) 打包提供(请参阅下面的订购信息)。

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関連項目

Nanog抗体(C-4)是一种IgG1 κ小鼠单克隆Nanog抗体(也被称作Nanog同源框抗体,或Nanog基因产物抗体),它通过WB、IP、IF和ELISA检测方法检测小鼠来源的Nanog蛋白。Nanog抗体(C-4)既可以作为非偶联形式的抗Nanog抗体使用,也可以作为多种偶联形式的抗Nanog抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Nanog(源自"Tir Na Nog",即神话中凯尔特人的永恒青春之地)是一种不同的同源结构域蛋白,指导未分化胚胎干细胞的多能性和分化。Nanog mRNA存在于多能的小鼠和人类细胞系中,但不存在于分化细胞中。人类Nanog蛋白与小鼠蛋白的整体氨基酸同一性为52%,在同源结构域中的同一性为85%。人类Nanog定位于基因位点12p13.31,而小鼠Nanog定位于基因位点6 F2。小鼠胚胎Nanog的表达在囊胚的内细胞团中被检测到。在未分化的NTERA-2 cl.D1胚胎癌细胞系中,通过Northern分析检测到了高水平的人类Nanog表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Nanog 抗体 (C-4)

sc-376915
200 µg/ml
$316.00

Nanog (C-4): m-IgG Fc BP-HRP 套装

sc-527551
200 µg Ab; 10 µg BP
$354.00

Nanog (C-4): m-IgG1 BP-HRP 套装

sc-532924
200 µg Ab; 20 µg BP
$354.00

Nanog 抗体 (C-4) AC

sc-376915 AC
500 µg/ml, 25% agarose
$416.00

Nanog 抗体 (C-4) HRP

sc-376915 HRP
200 µg/ml
$316.00

Nanog 抗体 (C-4) FITC

sc-376915 FITC
200 µg/ml
$330.00

Nanog 抗体 (C-4) PE

sc-376915 PE
200 µg/ml
$343.00

Nanog 抗体 (C-4) Alexa Fluor® 488

sc-376915 AF488
200 µg/ml
$357.00

Nanog 抗体 (C-4) Alexa Fluor® 546

sc-376915 AF546
200 µg/ml
$357.00

Nanog 抗体 (C-4) Alexa Fluor® 594

sc-376915 AF594
200 µg/ml
$357.00

Nanog 抗体 (C-4) Alexa Fluor® 647

sc-376915 AF647
200 µg/ml
$357.00

Nanog 抗体 (C-4) Alexa Fluor® 680

sc-376915 AF680
200 µg/ml
$357.00

Nanog 抗体 (C-4) Alexa Fluor® 790

sc-376915 AF790
200 µg/ml
$357.00

Can Nanog (C-4): sc-376915 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Nanog (C-4): sc-376915 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Didn't bind at all.Used it on mouse ips - didn't bind at all. Tried 3 different dilutions, including the manufacturer's recommended concentration, didn't bind at all. Had GAPDH in the same experiment, and it stained very well.
Date published: 2017-10-11
Rated 5 out of 5 by from ResultUsed it on human ips - didn't bind at all. Tried 3 different dilutions, including the manufacturer's recommended concentration, didn't bind at all. Had Oct4 in the same experiment, and it stained very well.
Date published: 2017-07-28
Rated 5 out of 5 by from Positive specific nuclear staingingPositive specific nuclear stainging of Nanog was observed with minimal non specific background staining.
Date published: 2017-02-12
Rated 1 out of 5 by from Did not workUsed it on D3 mouse embryonic stem cell - didn't bind at all. Just show the same straining as isotype control. Tried 4 different dilutions, including the manufacturer's recommended concentration, no difference in either. Bought both the 488 and 647 conjugate, same deal. Had Sox2, Oct4 and SSEA-1 in the same experiment, and those stained very well. I am guessing it's probably this particular antibody is not good.
Date published: 2016-12-09
Rated 3 out of 5 by from Decent antiDecent anti-nanog antibody. Sometimes the signal was quite weak.
Date published: 2015-11-11
Rated 5 out of 5 by from Produced positive Western blot data of NanogProduced positive Western blot data of Nanog expression in F9 whole cell lysate. -SCBT QC
Date published: 2013-06-30
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Nanog抗体 (C-4) is rated 4.0 out of 5 by 6.
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