Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
myopalladin Double Nickase Plasmid (h): sc-417221-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- myopalladin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- myopalladin Double Nickase Plasmid (h) and myopalladin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MYPN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: myopalladin Antibody (72-J): sc-81810
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
myopalladin Double Nickase Plasmid (h) | sc-417221-NIC | 20 µg | $410.00 | |||
myopalladin Double Nickase Plasmid (h2) | sc-417221-NIC-2 | 20 µg | $410.00 |
Human MYPN encodes myopalladin, a sarcomeric scaffold protein enriched at the Z-disc and I-band where it organizes actin-associated structures and supports mechanical stability in striated muscle. Through interactions with cytoskeletal and signaling partners, myopalladin contributes to myofibrillogenesis, sarcomere maintenance, and mechanotransduction pathways that couple contractile activity to nuclear gene expression programs. Altered MYPN function has been linked to inherited cardiomyopathies and myopathies, making it a relevant target for studying muscle architecture, stress-responsive remodeling, and downstream transcriptional adaptations.
myopalladin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MYPN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MYPN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MYPN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MYPN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.