Date published: 2025-9-7

021-6093-6350

SCBT Portrait Logo
Seach Input

myocilin 抗体 (F-12): sc-137233

5.0(2)
寫評論提問

说明书
  • myocilin 抗体 F-12 是小鼠单克隆 IgG2b κ,myocilin抗体, 在12篇文献中引用,规格为200 µg/ml
  • 针对氨基酸240-370的图谱,在myocilin的内部区域,human起源
  • myocilin 抗体 (F-12) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 myocilin
  • 抗myocilin抗体(F-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取myocilin (F-12): sc-137233免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是myocilin Antibody (F-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与myocilin Antibody (F-12) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

myocilin抗体(F-12)是一种IgG2b κ小鼠单克隆myocilin抗体(也称为myocilin抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的myocilin蛋白。myocilin抗体(F-12)既有非偶联形式的抗myocilin抗体,也有多种偶联形式的抗myocilin抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Myocilin是一种在眼睛中表达的细胞外蛋白,包括视网膜、小梁网和睫状体。Myocilin可以在体内形成同源多聚体,也可以通过与纤连蛋白的Hep II结构域相互作用与ECM的组分结合。此外,myocilin与肌球蛋白调节轻链相互作用,肌球蛋白调节轻链是肌球蛋白运动蛋白复合物的组分。这种相互作用意味着myocilin在肌动球蛋白系统中发挥作用,将myocilin与小梁网(TM)的功能状态联系起来,而小梁网(TM)负责控制眼压(IOP)。小梁网功能的改变可能导致眼压升高和青光眼的发展,青光眼是失明的主要原因。Myocilin由MYOC(也称为TIGR)编码,MYOC基因位于1q24.3染色体的GLC1A位点,并且容易发生突变。MYOC基因的突变与原发性开角型青光眼(POAG)密切相关,原发性开角型青光眼是一种以视网膜神经节细胞进行性丧失为特征的致盲性疾病。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

myocilin 抗体 (F-12) 参考文献:

  1. Myocilin 基因 (Myoc) 的靶向破坏表明人类青光眼致病突变是功能增益。  |  Kim, BS., et al. 2001. Mol Cell Biol. 21: 7707-13. PMID: 11604506
  2. TIGR/MYOC 在小梁网细胞外基质中的体外定域以及与纤维连接蛋白的结合。  |  Filla, MS., et al. 2002. Invest Ophthalmol Vis Sci. 43: 151-61. PMID: 11773026
  3. 蛋白质与肌纤蛋白的相互作用  |  Wentz-Hunter, K., et al. 2002. Invest Ophthalmol Vis Sci. 43: 176-82. PMID: 11773029
  4. 肌球蛋白与人类小梁网细胞中的线粒体有关。  |  Wentz-Hunter, K., et al. 2002. J Cell Physiol. 190: 46-53. PMID: 11807810
  5. 正常和患有青光眼的灵长类动物视神经中肌球蛋白/TIGR的表达。  |  Ricard, CS., et al. 2001. Exp Eye Res. 73: 433-47. PMID: 11825016

订购信息

产品名称产品编号规格价格数量收藏夹

myocilin 抗体 (F-12)

sc-137233
200 µg/ml
$316.00

myocilin (F-12): m-IgGκ BP-HRP 套装

sc-521462
200 µg Ab, 40 µg BP
$354.00

myocilin (F-12): m-IgG2b BP-HRP 套装

sc-548906
200 µg Ab; 10 µg BP
$354.00

myocilin 抗体 (F-12) AC

sc-137233 AC
500 µg/ml, 25% agarose
$416.00

myocilin 抗体 (F-12) HRP

sc-137233 HRP
200 µg/ml
$316.00

myocilin 抗体 (F-12) FITC

sc-137233 FITC
200 µg/ml
$330.00

myocilin 抗体 (F-12) PE

sc-137233 PE
200 µg/ml
$343.00

myocilin 抗体 (F-12) Alexa Fluor® 488

sc-137233 AF488
200 µg/ml
$357.00

myocilin 抗体 (F-12) Alexa Fluor® 546

sc-137233 AF546
200 µg/ml
$357.00

myocilin 抗体 (F-12) Alexa Fluor® 594

sc-137233 AF594
200 µg/ml
$357.00

myocilin 抗体 (F-12) Alexa Fluor® 647

sc-137233 AF647
200 µg/ml
$357.00

myocilin 抗体 (F-12) Alexa Fluor® 680

sc-137233 AF680
200 µg/ml
$357.00

myocilin 抗体 (F-12) Alexa Fluor® 790

sc-137233 AF790
200 µg/ml
$357.00

The MYOC gene is highly homologous between mouse and human, I wonder this antibody can differentiate them, especially for transgenic mouse with human MYOC, thank you.

Asked by: docthaly
Thank you for your question. This antibody can not distinguish between mouse and human MYOC or myocilin proteins. The antibody is recommended for detection of mouse, rat and human myocilin proteins.
Answered by: Technical Support
Date published: 2020-04-21

Do you have a reference related to development of this antibody? Additionally, could you refer me to an example/reference for use of this antibody in ELISA? Thank you.

Asked by: Athena
Thank you for your question. This antibody is a Santa Cruz product and was developed in house. We do not have a published citation in ELISA that we are aware of, but here are two citations using it in Western blot (PMID: 24691439, 21821918). Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-10-09

What is the recommended starting dilution for Western blot for sc-137233: myocilin (F-12) monoclonal antibody?

Asked by: Randy McDonald
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_137233, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 104ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good for WBThe myocilin Antibody (F-12): sc-137233 provides good data for Western blot showing clear band with minimal background noise present.
Date published: 2017-01-17
Rated 5 out of 5 by from WB publishable data with mouse TM cellWB publishable data with mouse TM cell - SCBT Publication Review
Date published: 2013-09-20
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_zh_CN, sid_137233, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT
myocilin 抗体 (F-12) is rated 5.0 out of 5 by 2.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_zh_CN, sid_137233, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 136ms
  • REVIEWS, PRODUCT