Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MYH9 Double Nickase Plasmid (h): sc-401182-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MYH9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MYH9 Double Nickase Plasmid (h) and MYH9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MYH9. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MYH9 Double Nickase Plasmid (h) | sc-401182-NIC | 20 µg | $410.00 | |||
MYH9 Double Nickase Plasmid (h2) | sc-401182-NIC-2 | 20 µg | $410.00 |
MYH9 encodes non-muscle myosin IIA, an actin-dependent motor protein that generates contractile force and supports cytoskeletal organization. It contributes to processes including cell adhesion, migration, mechanotransduction, cytokinesis, and maintenance of epithelial and platelet morphology through regulation of actomyosin dynamics. MYH9 activity interfaces with Rho GTPase/ROCK signaling and focal adhesion pathways that coordinate stress fiber assembly and cortical tension. Dysregulation or mutation of MYH9 is associated with inherited MYH9-related disorders characterized by macrothrombocytopenia and variable extra-hematologic features, making it a widely used target for studying cytoskeletal disease mechanisms.
MYH9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MYH9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MYH9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MYH9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MYH9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.