Date published: 2025-9-6
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MYH7 Antibody (A4.951) - Western Blotting - Image 380634
MYH7 抗体 (A4. 951): sc-53090. A-10 (A) 和 A-673 (B) 全细胞裂解液中 MYH7 表达的 Western 印迹分析.
MYH7 抗体 (A4. 951): sc-53090. A-10 (A) 和 A-673 (B) 全细胞裂解液中 MYH7 表达的 Western 印迹分析.

MYH7 抗体 (A4.951): sc-53090

4.3(8)
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说明书
  • MYH7抗体(A4.951)是小鼠单克隆IgG1 κ, 在48篇文献中引用，规格为200 µg/ml
  • 对human来源的成人骨骼肌肌球蛋白的抗性
  • 抗-MYH7 抗体 (A4.951) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human, rabbit 和fish 来源的 myosin heavy chain 7
  • 抗MYH7抗体（A4.951）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MYH7 (A4.951): sc-53090的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 MYH7 抗体 (A4.951) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MYH7 抗体 (A4.951) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Myosin抗体，包括MRLC2, MYH, MYH1, MYH10, MYH11, MYH15, MYH2, MYH3, MYH4和MYH6

MYH7抗体（A4.951）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠、人、兔和鱼来源的肌球蛋白重链7。抗肌球蛋白重链7抗体（A4.951）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。肌球蛋白重链7在肌肉收缩中起着至关重要的作用，主要存在于心脏和慢肌中，MYH7有助于产生力量和运动。肌球蛋白重链7的结构形成异六聚体，由两条重链和两对轻链组成，可与肌动蛋白丝进行适当的相互作用。这种结构安排能够提高肌肉收缩效率，并响应生理需求。MYH7基因的突变与肥厚性心肌病有关，这种疾病可导致严重的心脏并发症，这凸显了肌球蛋白重链7在健康和疾病中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MYH7 抗体 (A4.951) 参考文献:

  1. 人类和小鼠骨骼肌蛋白重链基因簇的组织结构高度保守。  |  Weiss, A., et al. 1999. Proc Natl Acad Sci U S A. 96: 2958-63. PMID: 10077619
  2. 松弛状态下肌球蛋白丝的原子模型。  |  Woodhead, JL., et al. 2005. Nature. 436: 1195-9. PMID: 16121187
  3. 肌球蛋白储存性肌病和肩胛腓骨肌病中的MYH7基因突变。  |  Pegoraro, E., et al. 2007. Neuromuscul Disord. 17: 321-9. PMID: 17336526
  4. 使用人类 cDNA 克隆作为探针，将人类肌球蛋白重链基因分配到 17 号染色体上。  |  Edwards, YH., et al. 1985. Ann Hum Genet. 49: 101-9. PMID: 3000272
  5. 人类横纹肌中表达两种不同形式的β肌球蛋白重链。  |  Jandreski, MA., et al. 1987. Hum Genet. 77: 127-31. PMID: 3653886
  6. 在 MYH7 E848G 肥厚型心肌病干细胞模型中，心肌细胞凋亡导致收缩功能障碍。  |  Loiben, AM., et al. 2023. bioRxiv.. PMID: 36747800
  7. 多尺度模型揭示了肥厚型心肌病MYH7 G256E突变导致心肌过度收缩和线粒体呼吸增强。  |  Lee, S., et al. 2023. bioRxiv.. PMID: 37333118
  8. 人类肌球蛋白重链基因的分离和特征描述。  |  Leinwand, LA., et al. 1983. Proc Natl Acad Sci U S A. 80: 3716-20. PMID: 6304733
  9. 小鼠和人类 DNA 中肉瘤肌球蛋白重链的多基因家族：在单条染色体上的定域。  |  Leinwand, LA., et al. 1983. Science. 221: 766-9. PMID: 6879174
  10. 人类骨骼肌 IIb 型纤维中表达 IIx 型肌球蛋白重链转录本。  |  Smerdu, V., et al. 1994. Am J Physiol. 267: C1723-8. PMID: 7545970
  11. 哺乳动物骨骼肌发育过程中依次表达的三条慢肌球蛋白重链  |  Hughes, SM., et al. 1993. Dev Biol. 158: 183-99. PMID: 7687223
  12. 在人类基因组中，五个骨骼肌蛋白重链基因组成了一个多基因复合体。  |  Soussi-Yanicostas, N., et al. 1993. Hum Mol Genet. 2: 563-9. PMID: 8518795

订购信息

产品名称产品编号规格价格数量收藏夹

MYH7 抗体 (A4.951)

sc-53090
200 µg/ml
$316.00

MYH7 (A4.951): m-IgG Fc BP-HRP 套装

sc-528547
200 µg Ab; 10 µg BP
$354.00

MYH7 (A4.951): m-IgGκ BP-HRP 套装

sc-520945
200 µg Ab, 40 µg BP
$354.00

MYH7 (A4.951): m-IgG1 BP-HRP 套装

sc-542987
200 µg Ab; 20 µg BP
$354.00

MYH7 抗体 (A4.951) AC

sc-53090 AC
500 µg/ml, 25% agarose
$416.00

MYH7 抗体 (A4.951) HRP

sc-53090 HRP
200 µg/ml
$316.00

MYH7 抗体 (A4.951) FITC

sc-53090 FITC
200 µg/ml
$330.00

MYH7 抗体 (A4.951) PE

sc-53090 PE
200 µg/ml
$343.00

MYH7 抗体 (A4.951) Alexa Fluor® 488

sc-53090 AF488
200 µg/ml
$357.00

MYH7 抗体 (A4.951) Alexa Fluor® 546

sc-53090 AF546
200 µg/ml
$357.00

MYH7 抗体 (A4.951) Alexa Fluor® 594

sc-53090 AF594
200 µg/ml
$357.00

MYH7 抗体 (A4.951) Alexa Fluor® 647

sc-53090 AF647
200 µg/ml
$357.00

MYH7 抗体 (A4.951) Alexa Fluor® 680

sc-53090 AF680
200 µg/ml
$357.00

MYH7 抗体 (A4.951) Alexa Fluor® 790

sc-53090 AF790
200 µg/ml
$357.00

What is the recommended starting dilution for Western blot for MYH7 (A4.951)?

Asked by: cjMara
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-33686.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 8
Date published: 2017-01-12

What is the exact epitope recognized by MYH7 (A4.951): sc-53090?

Asked by: cjMara
MYH7 Antibody (A4.951) is a mouse monoclonal raised against adult skeletal muscle myosin of human origin. The exact epitope has not been determined.
Answered by: Technical Support 8
Date published: 2023-03-06
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Rated 2 out of 5 by from not so good resultUsing IF, strong back ground appeared. conc: 0.4 μg~4 μg/ml
Date published: 2019-08-26
Rated 5 out of 5 by from Great signal for WBI bought this on Jan, 2018 for analyzing MYH7 in iPSC-CM. The result is great.
Date published: 2018-05-30
Rated 5 out of 5 by from dataGood antibody by WB(dilution 1/1000) on Human cancer cell.
Date published: 2017-04-05
Rated 4 out of 5 by from OkayNot as strong an antibody as the MYH7(A4.840) clone also available from santa cruz
Date published: 2017-01-17
Rated 5 out of 5 by from A great choice!This antibody can be expected to produce reliable detection of MYH7. Produces good WB data and even better immunostains. It's been cited several times by researchers.
Date published: 2016-12-23
Rated 5 out of 5 by from This has worked well in WBThis has worked well in WB.
Date published: 2015-10-21
Rated 5 out of 5 by from Good staining of myocytes in formalin fixedGood staining of myocytes in formalin fixed, paraffin-embedded human skeletal muscle tissue in IHC -SCBT QC
Date published: 2015-05-15
Rated 3 out of 5 by from Strong signal with background seen in WBStrong signal with background seen in WB with human skeletal muscle tissue extract. -SCBT QC
Date published: 2014-05-15
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MYH7 抗体 (A4.951) is rated 4.3 out of 5 by 8.
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